Staphylococcal cassette Chromosome mec Elements in Methicillin-Resistant Coagulase-Negative Staphylococci From a Brazilian Neonatal Care Unit

Salgueiro, Vivian C.; Azevedo, Milena Borgo; Iório, Natalia Lopes Pontes; Amorim, Efigênia de Lourdes Teixeira; Santos, Kátia Regina Netto dos

doi:10.1097/inf.0000000000000440

Cited by 5 publications

(6 citation statements)

References 10 publications

(5 reference statements)

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“…In this context, once again S. haemolyticus stands out, having the highest proportion (47%) of MDR strains among all MR-CoNS species identified in this study. This data highlights S. haemolyticus as an important CoNS associated with MDR phenotype, which has been pointed out in the literature in the last decades 5 , 13 , 27 , 29 , with reported MDR prevalence as high as 75.3% among this species 26 .…”

Section: Discussionsupporting

confidence: 67%

“…However, considering the possibility of a large variability of SCC mec elements in CoNS, applying SCC mec typing methods for this group becomes challenging, mostly because such methods were established for S. aureus . MRS-CoNS can either present new combinations of mec and ccr complex genes or present more than one mec and ccr complex in a single strain 29 , 32 – 34 .…”

Section: Discussionmentioning

confidence: 99%

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Increase in methicillin-resistant Staphylococcus spp. colonization among pregnant individuals during COVID-19 pandemic

Rio-Tinto,

Costa,

Alvim

et al. 2024

Sci Rep

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Methicillin-resistant Staphylococcus (MRS) has been associated with neonatal infections, with colonization of the anovaginal tract being the main source of vertical transmission. The COVID-19 pandemic has altered the frequency of antibiotic usage, potentially contributing to changes in the dynamics of bacterial agents colonizing humans. Here we determined MRS colonization rates among pregnant individuals attending a single maternity in Rio de Janeiro, Brazil before (January 2019–March 2020) and during (May 2020–March 2021) the COVID-19 pandemic. Anovaginal samples (n = 806 [521 samples before and 285 during the pandemic]) were streaked onto chromogenic media. Colonies were identified by MALDI-TOF MS. Detection of mecA gene and SCCmec typing were assessed by PCR and antimicrobial susceptibility testing was done according to CLSI guidelines. After the onset of the pandemic, MRS colonization rates increased significantly (p < 0.05) from 8.6% (45) to 54.7% (156). Overall, 215 (26.6%) MRS isolates were detected, of which S. haemolyticus was the most prevalent species (MRSH, 84.2%; 181 isolates). SCCmec type V was the most frequent among MRS (63.3%; 136), and 31.6% (68) of MRS strains had a non-typeable SCCmec, due to new combinations of ccr and mecA complexes. Among MRS strains, 41.9% (90) were resistant to at least 3 different classes of antimicrobial agents, and 60% (54) of them were S. haemolyticus harboring SCCmec V. MRS colonization rates and the emergence of multidrug-resistant variants detected in this study indicate the need for continuing surveillance of this important pathogen within maternal and child populations.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Increase in methicillin-resistant Staphylococcus spp. colonization among pregnant individuals during COVID-19 pandemic

Rio-Tinto,

Costa,

Alvim

et al. 2024

Sci Rep

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“…It should be noted that neonates are very vulnerable and frequently handled by health professionals. In addition, the detection of CoNS species colonizing neonates is common [23] and, the same pattern of Staphylococcus species is found as cause of bloodstream infections [10], highlighting the importance of the colonization as risk factor in the development of infections by Staphylococcus species.…”

Section: Discussionmentioning

confidence: 97%

“…They are responsible for 10% to 40% of the bloodstream infections (BSI) [1][2][3]6], and a high mortality rate of 46% was already associated to infections by CoNS in an intensive care unit [3]. Among neonates, S. aureus and CoNS are responsible for 18% and 54% of late onset sepsis, respectively [9], and Staphylococcus epidermidis and Staphylococcus haemolyticus are the most frequently isolated CoNS species [10].…”

Section: Introductionmentioning

confidence: 99%

High rate of neonates colonized by methicillin-resistant Staphylococcus species in an Intensive Care Unit

Salgueiro

Seixas

Guimarães

et al. 2019

J Infect Dev Ctries

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Introduction: Staphylococcal colonization is a risk factor for healthcare-associated infections, which are frequent in Neonatal Intensive Care Units (NICU). This study analyzed microbiology, epidemiology and clinical aspects of Staphylococcus spp. colonizing neonates. Methodology: Nasal or periumbilical swabs were evaluated from 175 newborns admitted to a NICU of a Rio de Janeiro hospital from March to September 2009. Clinical data were obtained from the medical records. SCCmec typing and the mecA and Panton-Valentine Leukocidin (PVL) genes were detected by PCR. Clonal diversity was evaluated by pulsed-field gel electrophoresis. Results: Staphylococcus spp. isolates were detected in 98 (56%) neonates, 66.3% of them had birth weight ≤ 2500 g, 62.2% were preterm (˂ 37 weeks) and the mean length of hospitalization was 14.9 days. Among the 133 isolates identified, 48.1% were S. epidermidis, 23.3% S. haemolyticus and 13.5% S. aureus. Methicillin-resistant Staphylococcus isolate was detected in 77.6% of neonates. The methicillin-resistant S. aureus isolates carried the SCCmec type IV, while 94.6% of S. epidermidis and 85.7% of S. haemolyticus presented non-typeable cassettes. Among the S. aureus, 55.6% had PVL genes and the USA800 genotype was prevalent. Two genotypes of S. epidermidis and one of S. haemolyticus clustered 42.2% and 25.8% of the isolates, respectively. S haemolyticus colonization was associated with the use of parenteral nutrition and mechanical ventilation. Conclusion: High rate of neonates colonized by methicillin-resistant Staphylococcus species and the permanence of clones circulating in the NICU highlight the importance for continuous and preventive surveillance in this high-risk population.

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“…Eleven types (I to XI) of SCC mec have been assigned for Staphylococcus aureus based on the classes of the mec gene complex and the types of the ccr gene complex [16]. In S. epidermidis these elements are very diverse and most of the isolates are defined as non-typeable [17–19]. …”

Section: Introductionmentioning

confidence: 99%

Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates

et al. 2017

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Background Staphylococcus epidermidis is an opportunistic pathogen involved in hospital-acquired infections, particularly in those related to medical devices. This study characterized 50 genetically unrelated S. epidermidis isolates from bloodstream infections (BSIs, n = 31) and nares (n = 19) of neonates in relation to staphylococcal chromosomal cassette mec (SCCmec) type, biofilm production and associated genes, and the arginine catabolic mobile elements (ACME), in order to detect virulence factors that could discriminate a potential invasiveness isolate or predict an increasing pathogenicity.ResultsIsolates from both groups showed no difference for biofilm production and ACME genes detection. However, BSI isolates harbored more frequently the sdrF and sesI genes (p < 0.05), whereas biofilm producer isolates were associated with presence of the aap gene. The sdrF gene was also significantly more in the biofilm producer isolates from BSI. The SCCmec type IV and the ccr2 complex were related to BSI isolates (p < 0.05), while 83% of the nasal isolates were non-typeable for the SCCmec elements, with the mec complex and ccr undetectable as the most frequent profile.ConclusionsDespite the great clonal diversity displayed by S. epidermidis isolates from neonates, BSI isolates harbored more frequently the sdrF and sesI adhesin genes, while nasal isolates were very variable in SCCmec composition. These aspects could be advantageous to improve colonization in the host increasing its pathogenicity.

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Staphylococcal cassette Chromosome mec Elements in Methicillin-Resistant Coagulase-Negative Staphylococci From a Brazilian Neonatal Care Unit

Cited by 5 publications

References 10 publications

Increase in methicillin-resistant Staphylococcus spp. colonization among pregnant individuals during COVID-19 pandemic

Increase in methicillin-resistant Staphylococcus spp. colonization among pregnant individuals during COVID-19 pandemic

High rate of neonates colonized by methicillin-resistant Staphylococcus species in an Intensive Care Unit

Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates

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