Standardized Methods for in-House Separation of a Blood Product for Multiple Use

Fujita, Hiroshi; Okuda, Makoto; Ohara, Akira; Kajiwara, Michiko; Koyama, Norihisa; Takano, Hisayo; Hosono, Shigeharu; Matsuzaki, Koji; Yazawa, Yurika; Maeda, Hiroo; Miyata, Shigeki

doi:10.3925/jjtc.62.673

Cited by 6 publications

(4 citation statements)

References 11 publications

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“…A pack of 480 ml FFP was thawed using a recirculating‐water‐bath thawing system at 37°C (FF‐40, Kawasumi Laboratories Incorporated, Oita, Japan). After thawing, 480 ml of FFP was separated into two blood bags having a capacity of 600 ml (KBP‐66 DC, Kawasumi Laboratories Incorporated, Oita, Japan), with 240 ml plasma dispensed in each bag, using a method as previously reported 9 . Two packs of the thawed plasma were re‐frozen at −80°C for 18 h and stored at −40°C until further experimentation.…”

Section: Methodsmentioning

confidence: 99%

“…After thawing, 480 ml of FFP was separated into two blood bags having a capacity of 600 ml (KBP-66 DC, Kawasumi Laboratories Incorporated, Oita, Japan), with 240 ml plasma dispensed in each bag, using a method as previously reported. 9 Two packs of the thawed plasma were re-frozen at −80°C for 18 h and stored at −40°C until further experimentation. We performed the experiments using the divided plasma from the same donor to compare the differences between the original standard CRYO preparation and the novel, modified thaw-siphon CRYO preparation (B-siphon method).…”

Section: Source and Production Of Blood Productsmentioning

confidence: 99%

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A novel modified thaw‐siphon method for extracting cryoprecipitate: The Bokutoh‐siphon method

et al. 2021

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Background Cryoprecipitate (CRYO) is neither produced nor supplied by the Japanese Red Cross Society. A novel CRYO extraction method established in‐house by modifying a thaw‐siphon technique was demonstrated in this study. Study Design and Methods A pack of fresh frozen plasma was thawed and equally divided into two bags for CRYO extraction by different methods. CRYO was extracted from the blood plasma using a standard centrifugation method and our modified thaw‐siphon method (Bokutoh‐siphon method; B method). The two different CRYOs extracted were analyzed to compare the differences in the amount of fibrinogen recovered, clotting factors extracted, and clotting activity. Results The amount of fibrinogen in the CRYO extracted using the B‐siphon method was similar to that obtained using the standard method (recovery of fibrinogen: B‐siphon method: 71.2% vs. standard method: 61.0%). The amount of clotting XIII factor extracted using the B‐siphon method was significantly lower than those extracted using the standard method. On the other hand, clotting II, V factors, and C1q esterase inhibitor not concentrated in CRYO content from the B‐siphon method were significantly higher than that from the standard method. Conclusion A new in‐house CRYO preparation method was established by modifying a previously used thaw‐siphon method. A coagulation factor‐rich CRYO was extracted from plasma frozen at −40°C along with the first fraction of thawed plasma, without using a large‐capacity refrigerated centrifuge for blood bags.

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Section: Methodsmentioning

confidence: 99%

Section: Source and Production Of Blood Productsmentioning

confidence: 99%

A novel modified thaw‐siphon method for extracting cryoprecipitate: The Bokutoh‐siphon method

et al. 2021

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“…In Japan, the Red Cross Blood Center does not supply RBC solutions in small‐sized bags. Therefore, small‐sized RBC bags are usually prepared in house …”

Section: Methodsmentioning

confidence: 99%

“…Two units of RBC solution (280 mL) were divided into 4 small volume–separated packs (BB‐TQ009J, Terumo Corp.) using a sterile connection device (TSCD‐II, Terumo Corp.) . Six parent RBC bags (numbered from 1 to 6) were tested in this study (Table ).…”

Section: Methodsmentioning

confidence: 99%

Use of potassium adsorption filter for the removal of ammonia and potassium from red blood cell solution for neonates

et al. 2018

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Standardized Methods of Potassium Adsorption Filter for in-House Separation of a Blood Product for Multiple Use

Fujita¹,

Igarashi²,

Okuda

et al. 2019

JJTC

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Standardized Methods for in-House Separation of a Blood Product for Multiple Use

Cited by 6 publications

References 11 publications

A novel modified thaw‐siphon method for extracting cryoprecipitate: The Bokutoh‐siphon method

A novel modified thaw‐siphon method for extracting cryoprecipitate: The Bokutoh‐siphon method

Use of potassium adsorption filter for the removal of ammonia and potassium from red blood cell solution for neonates

Standardized Methods of Potassium Adsorption Filter for in-House Separation of a Blood Product for Multiple Use

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