Standardized ileal digestible amino acids and apparent metabolizable energy content in defatted black soldier fly larvae meal fed to broiler chickens

Abstract

Standardized ileal digestibility (SID) of amino acids (AA) and apparent metabolizable energy corrected for nitrogen (AMEn) in defatted black soldier fly larvae meal (BSFLM) were determined in broiler chicks. A total of 180-d-old male broiler chicks (Ross 708) were fed a commercial broiler starter diet to day 13 of age. On day 14, birds were weighed and placed in cages (10 birds per cage; n = 6) and allocated one of two semi-purified cornstarch-based diets. The diets were nitrogen-free diet (NFD) for estimating endogenous AA losses and 20% crude protein test diet with BSFLM as the sole source of AA. All diets had 0.5% titanium dioxide (TiO 2 ) as an indigestible marker and the ratio of cornstarch to sucrose and soy oil in the test diet was identical to NFD to calculate AMEn by difference method. Birds were given feed and water ad libitum. Excreta samples were collected on days 17-20 and ileal digesta on day 21. The SID of lysine, methionine, cysteine, threonine, isoleucine, and valine was 86.3%, 88.7%, 72.8%, 85.5%, 89.6%, and 88.6%, respectively. Apparent retention of gross energy in BSFLM was 64.5% ± 2.27% and AMEn was 2902 ± 101 kcal kg −1 dry matter. The data will aid in accurate incorporation of BSFLM in poultry feeding programs.Key words: broilers, black soldier fly larvae meal, standardized ileal digestibility of amino acids, apparent metabolizable energy corrected for nitrogen.Résumé : La digestibilité iléale standardisée (SID -« standardized ileal digestibility ») des acides aminés (AA -« amino acids ») et l'énergie métabolisable apparente corrigée pour l'azote (AMEn -« apparent metabolizable energy corrected for nitrogen ») dans la moulée de larves de mouche soldat noire dégraissée (BFSLM -« black soldier fly larvae meal ») ont été déterminées chez les poussins à griller. Un total de 180 poussins de poulets à griller (Ross 708) mâles âgés d'un jour ont reçu une diète commerciale de départ pour les poulets à griller jusqu'au jour 13 d'âge. Au jour 14, les oiseaux ont été pesés et placés dans des cages (10 poussins par cage; n = 6) et assignés à l'une de deux diètes semi-purifiées à base d'amidon de maïs. Les diètes étaient : une diète sans azote (NFD -« nitrogen-free diet ») pour l'estimation des pertes endogènes d'AA et une diète test avec 20 % de protéines brutes avec le BSFLM comme seule source d'AA. Toutes les diètes comprenaient 0,5 % dioxyde de titane (TiO 2 ) comme marqueur indigeste et le rapport d'amidon de maïs au sucrose et à l'huile de soja dans la diète test était identique à la diète NFD pour pouvoir calculer l'AMEn par la méthode de différence. Les poulets ont reçu les aliments et l'eau ad libitum. Les échantillons d'excrétions ont été collectés des jours 17 à 20 et les digestas iléales au jour 21. La SID des AA lysine, méthionine, cystéine, thréonine, isoleucine et valine étaient de 86,3 %, 88,7 %, 72,8 %, 85,5 %, 89.6 % et 88,6 %, respectivement. La rétention apparente d'énergie brute dans le BSFLM était de 64,5 % ± 2,27 % et l'AMEn était de 2902 ± 101 kcal kg −1 de matières sèches. Ces ...

Effect of Alternative Protein Feeds on the Content of Selected Endogenous Bioactive and Flavour-Related Compounds in Chicken Breast Meat

“…On the other hand, in the case of soybean meal, the AID of histidine appeared to be comparable to that of the other protein sources [28]. Previous studies have also shown that the dietary levels of β-alanine influence the content of carnosine in muscle [18,27,28]. However, the absence of data regarding the β-alanine content in the three diets prevented us from making conclusions.…”

“…This observation suggests that factors other than the content of histidine in the feed could play a role in the final carnosine levels in meat. For example, in previous studies [2,27] it has been observed that the apparent ileal digestability (AID) of histidine in black soldier fly larva meal for broilers is lower than the one observed for the other amino acids. On the other hand, in the case of soybean meal, the AID of histidine appeared to be comparable to that of the other protein sources [28].…”

Standardized ileal digestibility of amino acids and apparent metabolizable energy in corn and soybean meal for organic broiler chicken production in Ontario

“…A N-free diet (NFD; wheat-starch-based diet) was formulated to allow estimation of endogenous N losses for the calculation of SID of AA (Adeola et al 2016). The test diets were designed such that the ratio of wheat starch to sucrose to soy oil (the sole sources of energy in NFD) was maintained constant to allow determination of AMEn in ingredients using the substitution method as previously described by Mwaniki and Kiarie (2018). In total, six semi-purified wheat-starch-based test diets were prepared: (1) CC, (2) local organic corn (OCL), (3) imported organic corn (OCI), (4) conventional SBM (CSBM), (5) local organic SBM (OSBML), and (6) imported organic SBM (OSBMI) ( Table 2).…”

“…The SID of AA in test samples and apparent retention (AR) of GE were calculated according to Adeola et al (2016). The AR of GE in test samples was determined by substitution method (Woyengo et al 2010;Mwaniki and Kiarie 2018) with NFD as the basal diet using the following equation:…”

“…The apparent metabolizable energy (AME) and AMEn content for test sample were calculated as previously described by Mwaniki and Kiarie (2018).…”