Standardization of oocyte size during artificial fertilization and optimization of stocking density during indoor larval and outdoor nursery rearing of captive spotted scat (Scatophagus argus) for a viable juvenile production system

Mandal, Babita; Kailasam, M.; Bera, Aritra; Sukumaran, K K; Hussain, Tanveer; Biswas, Gouranga; Vijayan, Koyadan Kizhakedath

doi:10.1016/j.aquaculture.2020.736262

Cited by 11 publications

(7 citation statements)

References 44 publications

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“…The net weight gain was estimated as 5.05±0.35 g, 6.14±0.42 g and 4.74±0.43 g in T1, T2 and T3, respectively. A similar trend of weight gain (3.48±1.20 g) was also observed during the outdoor fry rearing phase (30-60 days) at the stocking density of 80 scats.m -2 (Mandal et al 2021). Similarly, T2 with a stocking density of 100 fry.m -2 showed a considerably higher (p<0.05) performance in terms of RGR and SGR.…”

Section: Discussionsupporting

confidence: 75%

Optimization of stocking density of Spotted Scat (Scatophagus argus) in brackishwater nursery

Ahmmed

Naser

ISLAM

et al. 2023

Bangladesh J. Fish.

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Section: Discussionsupporting

confidence: 75%

Optimization of stocking density of Spotted Scat (Scatophagus argus) in brackishwater nursery

Ahmmed

Naser

ISLAM

et al. 2023

Bangladesh J. Fish.

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“…Spotted scat’s market value is relatively high due to its delicious taste. Although there are reports of artificial reproduction in spotted scat, the efficiency is still low in practice ( Mandal et al, 2021 ). The cultured spotted scat fry were mainly wildly caught in China.…”

Section: Introductionmentioning

confidence: 99%

Expression and regulation of 42Sp50 in spotted scat (Scatophagus argus)

et al. 2022

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42Sp50 is an isoform of the eukaryotic translation elongation factor 1 A (eEF1A) and is vital for fish ovarian development. Spotted scat (Scatophagus argus) is a popular marine cultured fish species in Southern Asia and China, and its artificial reproduction is complicated, with a relatively low success ratio in practice. In this study, the 42Sp50 gene was cloned from spotted scat. Tissue distribution analysis showed that 42Sp50 was mainly expressed in the ovary. qRT-PCR showed that 42Sp50 expression levels gradually decreased insignificantly in the ovaries from phase II to IV. Western blot analysis showed that 42Sp50 was highly expressed in the ovary, while it was almost undetectable in the testis. Immunohistochemistry analysis stained 42Sp50 mainly in the cytoplasm of the previtellogenic oocytes in ovaries of normal XX-female and sex-reversed XY-female. Aside from fish and amphibians, 42Sp50 was also identified in some reptile species using genomic database searching. Analyses of the transcriptome data from four different fish species (Hainan medaka (Oryzias curvinotus), silver sillago (Sillago sihama), Nile tilapia (Oreochromis niloticus), and Hong Kong catfish (Clarias fuscus)) revealed ovaries biased expression of 42Sp50 in all, similar to spotted scat. While the neighbor genes of 42Sp50 did not show ovary biased expression in the fish species analyzed. Bisulfite Sequencing PCR (BSP) results showed that the DNA methylation level of 42Sp50 promoter was low in ovaries, testes, and muscles. The luciferase reporter assay demonstrated that Dmrt4 activated 42Sp50 expression in the presence of Sf1 or Foxh1. These results suggest that 42Sp50 may be involved in regulating the early phase oocytes development of spotted scat.

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“…It is also a popular aquaria fish due to its colorful appearance, strong adaptability, and easiness to be cultured indoors (Gupta, 2016). For these reasons, studies focusing on its reproduction biology are on the rise, including reproduction endocrinology (Jiang et al, 2017;Zhang et al, 2018;Mandal et al, 2020), sex determination and differentiation (Mustapha et al, 2018(Mustapha et al, , 2021He et al, 2019), and artificial breeding (Cai et al, 2010;Gandhi et al, 2014;Mandal et al, 2021). Its artificial breeding is successful in several countries, including China (Cai et al, 2010) and India (Mandal et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…For these reasons, studies focusing on its reproduction biology are on the rise, including reproduction endocrinology (Jiang et al, 2017;Zhang et al, 2018;Mandal et al, 2020), sex determination and differentiation (Mustapha et al, 2018(Mustapha et al, , 2021He et al, 2019), and artificial breeding (Cai et al, 2010;Gandhi et al, 2014;Mandal et al, 2021). Its artificial breeding is successful in several countries, including China (Cai et al, 2010) and India (Mandal et al, 2021). To obtain matured spotted scat broodstock, appropriate nutritional factors should be maintained to enhance gonad development and induce reproduction (Cai et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Liver Transcriptomic Analysis of the Effects of Dietary Fish Oil Revealed a Regulated Expression Pattern of Genes in Adult Female Spotted Scat (Scatophagus argus)

et al. 2021

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Despite the significance of n-3 long-chain polyunsaturated fatty acid (n-3 LC-PUFA) in fish oil in promoting the maturation of female broodstocks, the detailed mechanism of the effect on the expression of hepatic reproduction-related genes is still unclear. In this study, transcriptome sequencing was used to analyze the effect of the higher dietary n-3 LC-PUFA level on gene expression in the liver of adult females spotted scat. Two-year-old female spotted scat (average weight, 242.83 ± 50.90 g) were fed with diets containing 8% fish oil (FO) or 8% soybean oil (SO) for 40 days. The fatty acid profile in the serum, liver, and ovary was analyzed, and high proportions of n-3 LC-PUFA were observed in the FO group. The final average fish body weight and gonadosomatic index were similar between the FO and SO groups. The serum vitellogenin (Vtg) and hepatosomatic index (HSI) of the FO group were significantly higher and lower than that of the SO group, respectively. Comparatively, the liver transcriptome analysis showed 497 upregulated and 267 downregulated genes in the FO group. Among them, the expression levels of three estrogen-regulated genes (i.e., Vtga, Vtgb, and Zp4) were significantly higher in the FO than in the SO group. This expression pattern could be related to the upregulation of Hsd17b7 (the key gene for the synthesis of liver steroid hormone) and the downregulation of the Hsp90 (the estrogen receptor chaperone). The expression levels of Foxo1a and Lep, which are involved in the lipid metabolism, decreased significantly in the FO group, which may be related to the lower level of HSI in the FO group. The genes related to liver LC-PUFA absorption and transport, Fabp2 and Mfsd2ab, were significantly upregulated in the FO group, indicating that fish actively adapt to high-fish-oil diets. In brief, high-fish-oil diets can influence the expression of genes related to liver n-3 LC-PUFA metabolism and reproduction, inhibit the accumulation of liver fat, and promote the liver health and gonad development. This study will contribute to clarifying the mechanism of dietary n-3 LC-PUFA on promoting reproductive development in teleost fish.

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Standardization of oocyte size during artificial fertilization and optimization of stocking density during indoor larval and outdoor nursery rearing of captive spotted scat (Scatophagus argus) for a viable juvenile production system

Cited by 11 publications

References 44 publications

Optimization of stocking density of Spotted Scat (Scatophagus argus) in brackishwater nursery

Optimization of stocking density of Spotted Scat (Scatophagus argus) in brackishwater nursery

Expression and regulation of 42Sp50 in spotted scat (Scatophagus argus)

Liver Transcriptomic Analysis of the Effects of Dietary Fish Oil Revealed a Regulated Expression Pattern of Genes in Adult Female Spotted Scat (Scatophagus argus)

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