Standardization of Inactivated H5N2 Influenza Vaccine and Efficacy against Lethal A/Chicken/Pennsylvania/1370/83 Infection

Jm, Wood; Kawaoka, Yoshihiro; La, Newberry; Bordwell, Elizabeth; Rg, Webster

doi:10.2307/1590681

Cited by 54 publications

(30 citation statements)

References 5 publications

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“…Measuring the HA protein content is the most direct method, but is a cumbersome and relatively insensitive process, requiring growth of large quantities of virus, concentration by ultracentrifugation and assaying with the radial single immunodiffusion test (Wood et al ., 1985;Swayne et al ., 1999). Haemagglutinating units have been used as an indirect measurement of HA protein content, but correlate poorly with HA protein content, while infectious titre has good correlation with HA content (Swayne et al ., 1999).…”

Section: Discussionmentioning

confidence: 99%

Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virus

2006

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High-pathogenicity (HP) avian influenza (AI) virus of the H5N1 subtype has caused an unprecedented epizootic in birds within nine Asian countries/regions since it was first reported in 1996. Vaccination has emerged as a tool for use in managing the infection in view of future eradication. This study was undertaken to determine whether two divergent H5N2 commercial vaccine strains, one based on a European and the other a North American low-pathogenicity AI virus, could protect chickens against a recent Asian H5N1 HPAI virus. The North American and European vaccine viruses had 84 and 91% deduced amino acid sequence similarity to the HA1 segment of haemagglutinin protein of Indonesia H5N1 HPAI challenge virus, respectively. Both vaccine strains provided complete protection from clinical signs and death. The vaccines reduced the number of chickens infected and shedding virus from the respiratory and intestinal tracts at the peak of virus replication. In addition, the quantity of virus shed was reduced by 10 4 to 10 5 median embryo infectious doses. The use of specific neuraminidase inhibition tests allowed identification of infected chickens within the vaccinated groups. These data indicate that the currently available H5 vaccines of European and North American lineages will protect chickens against the Asian H5N1 HPAI virus and reduce environmental contamination by the H5N1 HPAI virus. They will be an adjunct to biosecurity measures to reduce virus transmission.

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Section: Discussionmentioning

confidence: 99%

Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virus

2006

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“…After incubation at 4°C for 3 days, inactivation was confirmed by the absence of detectable infectivity after two blind passages of the formalin-treated allantoic fluid in 10-day-old embryonated chicken eggs. For the recombinant Ck/VN/C58/04 (H5N1) virus, the content of the HA protein in the allantoic fluid was standardized using a single radial immunodiffusion technique without concentration and purification (49). For the other recombinant viruses, the allantoic fluid was concentrated using a Pellicon-2 concentrator (Millipore Corporation, Bedford, MA) and purified using sucrose density gradient separation.…”

Section: Virusesmentioning

confidence: 99%

Pathogenicity and Vaccine Efficacy of Different Clades of Asian H5N1 Avian Influenza A Viruses in Domestic Ducks

Kim

Seiler

Forrest

et al. 2008

J Virol

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“…These results indicated that a strong B-cell memory response was generated after Salmonella HA DNA vaccination. The level of increase in HI titers after challenge can be a parameter to indicate the level of viral replication and accordingly, reflect the level of protection afforded by vaccination (35). Consistently, none of the chickens showed clinical signs and virus shedding in the SL(pHA5)ϩK group during the challenge period, but there was death and virus shedding in the SL(pHA5) and SL(p)ϩK groups (Table 2).…”

Section: Discussionmentioning

confidence: 75%

Prime-Boost Immunization Using a DNA Vaccine Delivered by Attenuated Salmonella enterica Serovar Typhimurium and a Killed Vaccine Completely Protects Chickens from H5N1 Highly Pathogenic Avian Influenza Virus

Pan

Zhang

Geng

et al. 2010

Clin Vaccine Immunol

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H5N1 highly pathogenic avian influenza virus (HPAIV) has posed a great threat not only for the poultry industry but also for human health. However, an effective vaccine to provide a full spectrum of protection is lacking in the poultry field. In the current study, a novel prime-boost vaccination strategy against H5N1 HPAIV was developed: chickens were first orally immunized with a hemagglutinin (HA) DNA vaccine delivered by attenuated Salmonella enterica serovar Typhimurium, and boosting with a killed vaccine followed. Chickens in the combined vaccination group but not in single vaccination and control groups were completely protected against disease following H5N1 HPAIV intranasal challenge, with no clinical signs and virus shedding. Chickens in the prime-boost group also generated significantly higher serum hemagglutination inhibition (HI) titers and intestinal mucosal IgA titers against avian influenza virus (AIV) and higher host immune cellular responses than those from other groups before challenge. These results demonstrated that the prime-boost vaccination strategy provides an effective way to prevent and control H5N1 highly pathogenic avian influenza virus.

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Standardization of Inactivated H5N2 Influenza Vaccine and Efficacy against Lethal A/Chicken/Pennsylvania/1370/83 Infection

Cited by 54 publications

References 5 publications

Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virus

Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virus

Pathogenicity and Vaccine Efficacy of Different Clades of Asian H5N1 Avian Influenza A Viruses in Domestic Ducks

Prime-Boost Immunization Using a DNA Vaccine Delivered by Attenuated Salmonella enterica Serovar Typhimurium and a Killed Vaccine Completely Protects Chickens from H5N1 Highly Pathogenic Avian Influenza Virus

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