Prime-Boost Immunization Using a DNA Vaccine Delivered by Attenuated
            <i>Salmonella enterica</i>
            Serovar Typhimurium and a Killed Vaccine Completely Protects Chickens from H5N1 Highly Pathogenic Avian Influenza Virus

Pan, Zhiming; Zhang, Xiaoming; Geng, Shizhong; Fang, Qiang; Meng, Yuchuan; Zhang, Lei; Jiao, Xinan; Liu, Xiufan

doi:10.1128/cvi.00387-09

Cited by 11 publications

(13 citation statements)

References 35 publications

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“…As for the choice of a booster, the inactivated vaccine is prepared from the whole virus particle, so the immune responses induced by inactivated IBV vaccines could respond to the whole virus. The inactivated vaccine has a better capability than choosing DNA plasmid as the booster to evoke memory B cells [16]. The inactivated vaccine as a booster is also considered to be able to balance the Th1 and Th2 immune responses [10], which could enhance both specific cell-mediated immunity and neutralizing antibody inductions with increased T cell response.…”

Section: Discussionmentioning

confidence: 99%

Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine

Fang

Zhao

et al. 2013

J Vet Sci

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The protective efficacy of DNA plasmids encoding avian infectious bronchitis virus (IBV) S1, N, or M protein was investigated in chickens. Chickens were inoculated monovalently (with plasmid pVAX1-16S1, pVAX1-16M, or pVAX1-16N alone) or multivalently (combination of the three different plasmids, pVAX1-16S1/M/N). A prime-boost immunization protocol against IBV was developed. Chickens were immunized with the multivalent DNA vaccine twice and then boosted with an inactivated vaccine once. Antibody titers of the chickens immunized with pVAX1-16S1/M/N were much higher than those of the monovalent groups (p < 0.01). A protective rate up to 90% was observed in the pVAX1-16S1/M/N group. The serum antibody titers in the prime-boost birds were significantly higher than those of the multivalent DNA vaccine group (p < 0.01) but not significantly different compared to the inactivated vaccine group at 49 days of age. Additionally, the prime-boost group also showed the highest level of IBV-specific cellular proliferation compared to the monovalent groups (p < 0.01) but no significant difference was found compared to the multivalent DNA vaccine group, and the prime-boost group completely protected from followed viral challenge.

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Section: Discussionmentioning

confidence: 99%

Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine

Fang

Zhao

et al. 2013

J Vet Sci

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“…The first DNA vaccine that was studied in poultry in 1993 was directed against AIV [19]. Thereafter DNA vaccination against AIV has been widely studied in chickens [6,[19][20][21][22][23][24][25][26][27][28], and more modestly in ducks [10] (Table 1). Promising results have been obtained for this model.…”

Section: Different Poultry Dna Vaccination Modelsmentioning

confidence: 99%

“…Oral vaccination was the second most popular route. In this case, the plasmids were either carried by attenuated bacteria such as Salmonella typhimurium [25,79] or Escherichia coli [60], or by silver nanoparticles [26]. More rarely, plasmid DNA was inoculated via IN instillations alone.…”

Section: The Different Administration Routes and Doses Of Dna Vaccinementioning

confidence: 99%

“…In turkeys, this regimen gave some protection against Chlamydophila psittaci infections [95]. Alternatively, booster immunizations with inactivated vaccines also gave good protection against AIV [25], IBDV [40] or IBV [37] infections. Furthermore, heterologous booster immunizations were also performed after in ovo DNA prime inoculations in IBDV [34,80], IBV [37] and Eimeria [65] models.…”

Section: Heterologous Prime-boost Strategiesmentioning

confidence: 99%

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DNA vaccination of poultry: The current status in 2015

Meunier

Chemaly

Dory

2016

Vaccine

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DNA vaccination is a promising alternative strategy for developing new human and animal vaccines. The massive efforts made these past 25 years to increase the immunizing potential of this kind of vaccine are still ongoing. A relatively small number of studies concerning poultry have been published. Even though there is a need for new poultry vaccines, five parameters must nevertheless be taken into account for their development: the vaccine has to be very effective, safe, inexpensive, suitable for mass vaccination and able to induce immune responses in the presence of maternal antibodies (when appropriate). DNA vaccination should meet these requirements. This review describes studies in this field performed exclusively on birds (chickens, ducks and turkeys). No evaluations of avian DNA vaccine efficacy performed on mice as preliminary tests have been taken into consideration. The review first describes the state of the art for DNA vaccination in poultry: pathogens targeted, plasmids used and different routes of vaccine administration. Second, it presents strategies designed to improve DNA vaccine efficacy: influence of the route of administration, plasmid dose and age of birds on their first inoculation; increasing plasmid uptake by host cells; addition of immunomodulators; optimization of plasmid backbones and codon usage; association of vaccine antigens and finally, heterologous prime-boost regimens. The final part will indicate additional properties of DNA vaccines in poultry: fate of the plasmids upon inoculation, immunological considerations and the use of DNA vaccines for purposes other than preventing infectious diseases.

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“…Influenza virus is made up of 11 proteins, and studies have shown that HA is no doubt the major target for generating protective immunity [11, 18]. Anti-HA antibodies have potential to block viral attachment and entry, and thus infection of the host cells subsequently [20].…”

Section: Introductionmentioning

confidence: 99%

A consensus-hemagglutinin-based vaccine delivered by an attenuated Salmonella mutant protects chickens against heterologous H7N1 influenza virus

2017

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H7N3 and H7N7 are highly pathogenic avian influenza (HPAI) viruses and have posed a great threat not only for the poultry industry but for the human health as well. H7N9, a low pathogenic avian influenza (LPAI) virus, is also highly pathogenic to humans, and there is a great concern that these H7 subtypes would acquire the ability to spread efficiently between humans, thereby becoming a pandemic threat. A vaccine candidate covering all the three subtypes must, therefore, be an integral part of any pandemic preparedness plan. To address this need, we constructed a consensus hemagglutinin (HA) sequence of H7N3, H7N7, and H7N9 based on the data available in the NCBI in early 2012-2015. This artificial sequence was then optimized for protein expression before being transformed into an attenuated auxotrophic mutant of Salmonella Typhimurium, JOL1863 strain. Immunizing chickens with JOL1863, delivered intramuscularly, nasally or orally, elicited efficient humoral and cell mediated immune responses, independently of the route of vaccination. Our results also showed that JOL1863 deliver efficient maturation signals to chicken monocyte derived dendritic cells (MoDCs) which were characterized by upregulation of costimulatory molecules and higher cytokine induction. Moreover, immunization with JOL1863 in chickens conferred a significant protection against the heterologous LPAI H7N1 virus challenge as indicated by reduced viral sheddings in the cloacal swabs. We conclude that this vaccine, based on a consensus HA, could induce broader spectrum of protection against divergent H7 influenza viruses and thus warrants further study.

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Prime-Boost Immunization Using a DNA Vaccine Delivered by Attenuated Salmonella enterica Serovar Typhimurium and a Killed Vaccine Completely Protects Chickens from H5N1 Highly Pathogenic Avian Influenza Virus

Cited by 11 publications

References 35 publications

Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine

Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine

DNA vaccination of poultry: The current status in 2015

A consensus-hemagglutinin-based vaccine delivered by an attenuated Salmonella mutant protects chickens against heterologous H7N1 influenza virus

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