Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’

Abstract

Micropropagation protocol of Oriental Hybrid Lilium cv. Ravenna was developed using bulb scale segments (Basal and Tip) as explants. Surface sterilization of healthy bulb scales with carbendazim 200 ppm for 30 min, then 0.1 percent mercuric chloride for 10 min, then 70% ethyl alcohol for 30 s was superior to all other treatments in recording highest culture asepsis (77.08%) and higher explant survival (86.12%). Explant survival was higher in basal segments (88.54%) compared to tip segments (85.52%). Highest culture establishment was recorded in basal scale segments (68.26%) followed by tip scale segments (55.21%). MS medium augmented with 0.50 mgl −1 Naphthalene acetic acid and 2.0 mgl −1 . 6-Benzylamino Purine recorded maximum culture establishment (76.17%), highest bulblet number/explant (5.52) with maximum length of shoots (2.20 cm) and number of leaves (3.39). This treatment combination of growth regulators resulted in highest shoot proliferation (83.33%) along with maximum shoot number (2.41explant −1 ), shoot length (2.35 cm) and leaf number (5.44) of micro shoots during proliferation stage. Rooting of explants was superior with Indole-3-butyric acid compared to Naphthalene acetic acid. Highest rooting of 92.71% along with maximum number of primary roots shoot −1 (12.06), maximum primary root length (3.17 cm) was documented in Murashige and Skoog medium added with Indole-3-butyric acid 1.50 mgl −1 with best ex vitro survival rate (98.96%) of rooted plantlets during primary hardening in perlite + vermiculite (1:1) mixture.

Standardization of in vitro micropropagation of Winter Jasmine (Jasminum nudiflorum) using nodal explants

“…Combined sterilant treatments aggravate the phyto-toxic effects leading to reduced explant survival. Similar results were recorded by Rather et al, 2014 , Rafiq et al, 2021 with underground buds of herbaceous peony and Oriental Lilium cv. Ravenna, respectively.…”

Advanced modeling and optimizing for surface sterilization process of grape vine (Vitis vinifera) root stock 3309C through response surface, artificial neural network, and genetic algorithm techniques

“…The sterilization technique is the most significant step for establishing successful in vitro plant regeneration in different crop species. [ [49] , [50] , [51] ]; Gu et al, 2022). Traditional optimization techniques focus on one specific Pareto-optimal solution at a time, transforming the multi-objective optimization problem into a single-objective problem by using multi-criterion decision-making techniques.…”

An Academic and Technical Overview on Plant Micropropagation Challenges

“…Selecting the proper protocol for plant micropropagation is also a crucial factor, which depends on the plant species. For standardization of the micropropagation protocol of any plant species, many studies have been recently reported (e.g., [155][156][157]). Are these challenges facing both developing and developed countries?…”