Standardization of Eclipta Alba by HPTLC, HPLC and AAS

Abstract

The whole plant of bhringaraj (Eclipta alba) was dried and pulverized. The phytoconstituents of the pulverized plant material were extracted separately with two different solvents namely petroleum ether (hot percolation) and methanol (cold maceration) to carry out a comparative extraction efficiency of the above two solvents. β-sitosterol reported to have antiandrogenic, anticancer, antiinflammatory, antiprostatitic, antitumor, etc. activity, was selected as the active biomarker for quantification of the aforementioned plant material.HPTLC was carried out for quantification of the biomarker and obtained data was compared with HPLC data. Petroleum ether was found to be an effective extracting solvent for β sitosterol from E. alba as compared to methanol. The percentage content of β-sitosterol in E. alba methanolic and petroleum ether extract was found to be 0.10% and 4.65% w/w respectively by HPTLC whereas the percentage content of β-sitosterol in Eclipta alba petroleum ether extract was found to be 4.67 % w/w by HPLC. AAS data revealed the presence of the metals (ppm ± SEM) are within safety limits, copper (1.151 ± 0.031), chromium (0.528 ± 0.012), cadmium (0.021 ± 0.035), lead (0.860 ± 0.009), arsenic (0.081 ± 0.007), mercury (0.036 ± 0.010).

Process Validation of Beta-Sitosterol Hair Gel Formulation and Evaluation of 5 Alpha Reductase Inhibition in Vitro for the Treatment of Androgenetic Alopecia

“…HPTLC method was used to quantify the β-sitosterol reported by Bhattacharjee et al (2017) with minor modifications [23]. The CAMAG HPTLC system consisting of WINCATS software, LINOMAT V automatic sample applicator, an automatic development chamber, scanning densitometer CAMAG scanner 3, and photo documentation apparatus CAMAG reprostar 3 were used.…”

“…HPLC method was performed to quantify the β-sitosterol reported by Bhattacharjee et al (2017) with some modifications [23]. RP-HPLC system (Shimadzu Prominence, Kyoto, Japan) equipped with two Shimadzu LC-20 UFLC reciprocating pumps, a variable Shimadzu SPD-M20A Prominence PDA detector and a rheodyne manual injector with a loop size of 20 μl was used.…”

Exploring the Potential of Eclipta alba: A Promising Approach for Hair Treatment Management through 5-Alpha Reductase Inhibition

“…12 Standardization of β-sitosterol in E. alba Extracts using HPTLC The approach described by Bhattacharjee et al (2017) has been modified in some respects. 13 CAMAG HPTLC system includes the scanning densitometer, LINOMAT V automated sample applicator, and automatic development chamber, with WINCATS software. The image documentation tools CAMAG Reprostar 3 and CAMAG Scanner 3 were utilized.…”