A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis

Khalid, Mohammad; Alqarni, Mohammed H.; Foudah, Ahmed I.; Alam, Prawez

doi:10.1007/s00764-021-00084-x

Cited by 4 publications

(1 citation statement)

References 21 publications

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“…To be specific quercetin, gallic acid, ellagic acid and ascorbic acid are present in polyherbal blend and known for its effect. Some methods are reported which includes HPTLC method for simultaneous estimation of quercetin and gallic acid in Leea indica [6], in Eclipta alba and Guiera senegalensis simultaneous HPTLC method is developed for estimation of quercetin and gallic acid [7], HPTLC simultaneous estimation of gallic acid and quercetin is also reported in single plant extract of Abutilon indicus [8] and HPTLC method is also reported in literature for estimation of gallic acid, rutin and quercetin in aqueous extract of Terminalia chebula [9] But simultaneous estimation of gallic acid and quercetin is not reported in polyherbal blend viz. three herbal plant mixture.…”

Section: Introductionmentioning

confidence: 99%

Validated High Performance Thin Layer Chromatography Method for Simultaneous Estimation for Gallic Acid and Quercetin in Polyherbal Blend and Their Quantitative Estimation

Gandhi¹,

Gawhane²,

Kapse³

et al. 2022

Bull. of the Kar. Univ. "Chem". Ser.

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Simple, sensitive high performance thin layer chromatography method for the estimation of gallic acid and quercetin in in-house polyherbal blend has been developed and validated. Methanolic solution of herbal blend comprising of Emblica officinalis, Camellia sinensis and Garcinia cambogia was used for analysis. The sepa-ration was performed on TLC aluminum plates precoated with silica gel G60 F254 and toluene : ethyl ace-tate : formic acid (5:1.5:1 v/v/v) at 254 nm scanning wavelength. The system gave well resolved peaks for gallic acid and quercetin at Rf 0.14 and Rf 0.29 respectively. The method validated as per ICH Q2R1 guide-lines which shows regression co-efficient 0.9939 for gallic acid and 0.9988 for quercetin in range of 2–6 μg/ml. Recovery of gallic acid and quercetin was found in range of 98–102 % which confirms the accuracy of method. Precision study (interday & intraday) showed that the relative standard deviation is less than 2 %, showing method is well precise. Proposed validated HPTLC method is simple, precise, specific, robust and accurate, and could find application in routine quality-control analysis. The method was used for quantitative estimation of gallic acid and quercetin in the polyherbal blend and was found as 1.648 % w/w and 3.165 % w/w respectively.

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