2010
DOI: 10.5487/tr.2010.26.3.203
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Standardization of Bronchoalveolar Lavage Method Based on Suction Frequency Number and Lavage Fraction Number Using Rats

Abstract: Bronchoalveolar lavage (BAL) is a useful tool in researches and in clinical medicine of lung diseases because the BAL fluid contains biochemical and cytological indicators of the cellular response to infection, drugs, or toxicants. However, the variability among laboratories regarding the technique and the processing of the BAL material limits clinical research. The aim of this study was to determine the suction frequency and lavage fraction number necessary to reduce the variability in lavage using male Sprag… Show more

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Cited by 24 publications
(17 citation statements)
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“…Our results likely underestimate the levels of shed MUC1-ED within the alveolar lining fluid (26) and the mucus blanket that covers the airway epithelium (27). Further, a dilutional effect is intrinsic to the BAL procedure (28), and some loss of protein is anticipated during MUC1-ED preparation (20).…”
Section: Muc1-ed Is a Shed Decoy Receptor In Vivomentioning
confidence: 82%
“…Our results likely underestimate the levels of shed MUC1-ED within the alveolar lining fluid (26) and the mucus blanket that covers the airway epithelium (27). Further, a dilutional effect is intrinsic to the BAL procedure (28), and some loss of protein is anticipated during MUC1-ED preparation (20).…”
Section: Muc1-ed Is a Shed Decoy Receptor In Vivomentioning
confidence: 82%
“…Bronchoalveolar lavage (BAL) was collected from each rat after three suctions, as described before [ 23 ]. The levels of TNF-α and IL-6 in BAL were measured using the ELISA kits for the corresponding cytokines (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions.…”
Section: Methodsmentioning
confidence: 99%
“…La primera muestra fue descartada, la segunda fue almacenada a 4 °C para el posterior análisis de lactato deshidrogenasa (LDH). La tercera muestra se utilizó para el recuento celular [27] .…”
Section: Lavado Broncoalveolar (Lba)unclassified