Standardization of a set of microsatellite markers for use in cultivar identification studies in olive (Olea europaea L.)

Doveri, Silvia; Gil, Fabíola Sabino; Díaz, Aurora; Reale, Silvia; Busconi, Matteo; Machado, Artur da Câmara; Martı́n, A.; Fogher, Corrado; Donini, Paolo; Lee, David

doi:10.1016/j.scienta.2008.02.005

Cited by 53 publications

(48 citation statements)

References 47 publications

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“…However, it is not generally observed, even using powerful genetic markers like SSRs, except in those regions where the genetic exchange has been very limited. Although the SSR technique has been used to compare the main Spanish cultivars with other Mediterranean ones (Diaz et al, 2006;Doveri et al, 2008;Bracci et al, 2009), no specific clustering among the main Spanish genotypes has been found. Most of the genetic diversity was found to be attributable to differences among genotypes within a country.…”

Section: Genetic Relationships Among Olive Cultivarsmentioning

confidence: 99%

“…Molecular methods for olive cultivar fingerprinting have been demonstrated to be effective, but microsatellite or simple sequence repeat (SSR) analysis is becoming the preferred choice for its high discriminatory power and simpler interpretation (Bracci et al, 2011). In an effort to trace the provenance of olive cultivars, recent reports have provided a list of recommended SSR markers and procedures for genotyping olive (Doveri et al, 2008;Baldoni et al, 2009). Also, the local germplasm for limited or small cultivation areas has recently been characterized by SSR markers, suggesting high levels of genetic diversity as well as notable variability of wild and cultivated types at the regional scale (Poljuha et al, 2008;Bracci et al, 2009;Belaj et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain

Delgado-Martinez¹,

Amaya²,

Sánchez-Sevilla³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8, with a mean of 6.25 alleles per primer pair (a total of 25 alleles). The observed heterozygosity ranged from 0.58 to 0.95, while the expected heterozygosity varied between 0.68 and 0.83. The polymorphism information content values ranged from 0.63 to 0.79. The mean polymorphism information content value of 0.70 for the SSR loci provided sufficient discriminating ability to evaluate the genetic diversity among the cultivars. The SSR data allowed unequivocal identification of all the cultivars; a combination of three SSR markers was sufficient to discriminate all 25 olive cultivars. A dendrogram was prepared, using the unweighted pair-group method with arithmetic mean clustering algorithm; it depicted the pattern of Genetic diversity of olive cultivars by microsatellite markers relationships between the cultivars. Most of the local cultivars grouped according to their geographic origin. No clear clustering trends were observed when the morphological traits of fruit endocarps or fruit use of cultivars were employed as analysis criteria. We conclude that there is a high level of variability among local olive cultivars from the Extremadura region at both the morphological and molecular levels; these data should be useful for identifying and distinguishing local germplasm.

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Section: Genetic Relationships Among Olive Cultivarsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain

Delgado-Martinez¹,

Amaya²,

Sánchez-Sevilla³

et al. 2012

Genet. Mol. Res.

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“…Therefore, it is vital for the genetic identity of olive germplasm to be verified before distribution to these users. Instances of misidentification or ambiguous identity, as with the 'Zitoum' example above, highlight the need for a standardized set of molecular markers used to build a database of verified olive cultivar genotypes (Doveri et al 2008;Baldoni et al 2009), a set harmonized with researchers in the Mediterranean and worldwide to ensure robust, globally accurate identities.…”

Section: Genetic Diversity and Cultivar Identitymentioning

confidence: 99%

Molecular characterization of genetic diversity, structure, and differentiation in the olive (Olea europaea L.) germplasm collection of the United States Department of Agriculture

Koehmstedt

Aradhya

Soleri

et al. 2010

Genet Resour Crop Evol

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Fifteen microsatellite loci were used to genotype 108 accessions of cultivated olive, Olea europaea L. ssp. europaea var. europaea, and eight of O. europaea L. ssp. cuspidata (Wall. ex G. Don) Ciferri, from the germplasm collection of the United States Department of Agriculture in Davis, California. Number of alleles per locus ranged from 3, for locus IAS-pOe12_A, to 16, for locus ssrOeUA-DCA11, with an overall mean of 9.93. Observed heterozygosity ranged from 0.175, for locus UDO99-019, to 0.937, for locus GAPU89, with a mean of 0.640. The cluster analysis using the Unweighted Pair Group Method using Arithmetic mean (UPGMA) method displayed thirteen clusters within seven main groups that can be partially described by common geographic origin or fruit use, though overlap among these groups was common. The locus-wise total gene diversity (H T ) ranged from 0.319, at UDO99-019, to 0.847, at ssrOeUA-DCA3, with an overall mean of 0.696. Most of the gene diversity was partitioned within clusters, with proportions (H S /H T ) ranging from 0.633, at IASpOe12_B, to 0.848 at GAPU89 per locus, with a mean of 0.759. The principal components analysis explained 24.8% of the total variation along the first two components. Projection of accessions onto the first two principal components produced affinities generally in agreement with the results of the UPGMA cluster analysis. The California cultivar 'Mission' clustered closely with Iberian cultivars and may represent clonal selections adapted to local growing conditions. The results show significant diversity but low levels of differentiation among olive cultivars within the collection.

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“…A list of recommended SSR markers and protocols for olive genotyping has been provided with the aim to develop a robust method to track the origin of olive cultivars (Doveri et al 2008;Baldoni et al 2009 Table 4. List of microsatellites isolated in olive which their related primers are available in the literature, most of them have been used in case of olive oil traceability (Bracci et al, 2011) For studying the informative potential of the microsatellites, the observed (Ho) and expected (He) heterozygosities generally are calculated using the software POPGENE ver.…”

Section: Simple Sequence Repeats (Ssrs)mentioning

confidence: 99%

Traceability of Origin and Authenticity of Olive Oil

Rabiei¹,

Enferadi²

2012

Olive Oil - Constituents, Quality, Health Properties and Bioconversions

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Standardization of a set of microsatellite markers for use in cultivar identification studies in olive (Olea europaea L.)

Cited by 53 publications

References 47 publications

Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain

Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain

Molecular characterization of genetic diversity, structure, and differentiation in the olive (Olea europaea L.) germplasm collection of the United States Department of Agriculture

Traceability of Origin and Authenticity of Olive Oil

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