Standardisation of primers and an algorithm for HIV-1 diagnostic PCR evaluated in patients harbouring strains of diverse geographical origin

Vandamme, Anne‐Mieke; Fransen, Katrien; Debaisieux, Laurent; Marissens, D.; Sprecher, S; Vaira, Dolorès; Vandenbroucke, Anne-Thérèse; Verhofstede, Chris; Dooren, Sonia Van; Goubau, Patrick; Desmyter, Jan; Beenhouwer, H. De; Groen, G van der; Piot, Peter; Liesnard, Corinne; Serruys-Schoutens, E.; Piérard, Denis; Lauwers, Sabine; Zissis, Georges; Miller, Kenneth R.; Clerc, J.Cogniaux-Le; Sondag-Thull, D; Burtonboy, Guy; Delferriere, Nicole; Emmelo, John van; Reniers, S.; Plum, Jean

doi:10.1016/0166-0934(94)00126-2

Cited by 66 publications

(37 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amplicons were analyzed by agarose gel electrophoresis (AGE) on 1.4% agarose and stained with ethidium bromide. All DNA samples were positive in a globin PCR, confirming that they were of sufficient quality for further PCR examination (Vandamme et al, 1995).…”

Section: Pcr For Hrv-5mentioning

confidence: 89%

See 1 more Smart Citation

Human retrovirus 5 sequences in peripheral blood cells of patients with B-cell non-Hodgkin's lymphoma

Murovska

Lejniece²,

Kozireva

et al. 2000

Int. J. Cancer

View full text Add to dashboard Cite

Section: Pcr For Hrv-5mentioning

confidence: 89%

“…DNA was extracted by using a standard phenol-chloroform method, precipitated with ethanol and dissolved in 50 -150 l of dH 2 O. A globin PCR (Vandamme et al, 1995) was carried out to control the quality of the extracted DNA.…”

Section: Dna Extractionmentioning

confidence: 99%

Human retrovirus 5 sequences in peripheral blood cells of patients with B-cell non-Hodgkin's lymphoma

Murovska

Lejniece²,

Kozireva

et al. 2000

Int. J. Cancer

View full text Add to dashboard Cite

“…PCR for determining HIV infection in neonates and children was performed as described elsewhere [Vandamme et al, 1995]. Each sample was tested with both the new and the reference antigen test.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a newly developed HIV antigen test

et al. 1997

Self Cite

View full text Add to dashboard Cite

A new monoclonal antibody-based enzyme immunoassay (Innogenetics) for the detection and quantification of p24 core antigens of HIV-1 (group M and group O) and of HIV-2 was evaluated on 2745 serum samples and 18 culture supernatants and compared with a reference (Coulter) HIV-1 p24 antigen assay. Positive results were confirmed by neutralization with the reagents of the respective tests. As demonstrated by dilution series of HIV cocultures, the new test recognizes p24 antigen of the most common HIV genetic subtypes, including group O and HIV-2. Titres ranged from 729 to 531441. Therefore p24 antigen assay is but very weakly reactive with HIV-2 (titres from 9 to 81). The new test is considerably more sensitive than the reference. In a population of 365 follow-up samples from 86 different patients, representing all stages of infection, the new test detected p24 antigen at least once in 52% (45/86) of these patients, whereas the reference was positive in 31% (27/86). The newly designed test detected antigen in 40% (145/365) of the samples, while the reference was positive in 21% (75/365). In a group of PCR and/or culture positive neonates, 33% (9/27) of the samples were positive with the new test versus 18% (5/27) with the reference. The specificity of the new test, as determined on 2,000 blood donor samples, was 99.65% (initially), 99.80% (after repetition), and 100% (with neutralization). The reference scored 99.95%, 100%, and 100%, respectively. In 300 seronegative samples from persons at risk, the initial specificity of the new test was 98.67% (the reference, 99.00%). With neutralization, both assays were 100% specific.

show abstract

“…A sample was considered indeterminate if a positive result was obtained for only one primer set and HIV negative if the results for both initial primer sets (pol and env) were negative (40).…”

Section: Vol 12 2005 Pediatric Hiv Screening In An African Hospital 87mentioning

confidence: 99%

Pediatric Human Immunodeficiency Virus Screening in an African District Hospital

Baets¹,

Edidi

Kasali³

et al. 2005

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

In order to evaluate alternative tests and strategies to simplify pediatric human immunodeficiency virus (HIV) screening at the district hospital level, a cross-sectional exploratory study was organized in the Democratic Republic of the Congo. Venous and capillary phlebotomies were performed on 941 Congolese children, aged 1 month to 12 years (153 children under 18 months and 788 children more than 18 months old). The HIV prevalence rate was 4.7%. An algorithm for children more than 18 months old, using serial rapid tests (Determine, InstantScreen, and Uni-Gold) performed on capillary blood stored in EDTA tubes, had a sensitivity of 100.0% (95% confidence interval [CI], 88.9 to 100.0%) and a specificity of 100.0% (95% CI, 99.5 to 100.0%). The results of this study suggest that the ultrasensitive p24 antigen assay may be performed on capillary plasma stored on filter paper (sensitivity and specificity, 100.0%; n ‫؍‬ 87) instead of venous plasma (sensitivity, 92.3%; specificity, 100.0%; n ‫؍‬ 150). The use of glucolets (instruments used to perform capillary phlebotomies), instead of syringes and needles, may reduce procedural pain and the risk of needle stick injuries at a comparable cost. Compared to the reference, HIV could have been correctly excluded based on one rapid test for at least 90% of these children. The results of this study point towards underutilized opportunities to simplify phlebotomy and pediatric HIV screening.

show abstract

Standardisation of primers and an algorithm for HIV-1 diagnostic PCR evaluated in patients harbouring strains of diverse geographical origin

Cited by 66 publications

References 17 publications

Human retrovirus 5 sequences in peripheral blood cells of patients with B-cell non-Hodgkin's lymphoma

Human retrovirus 5 sequences in peripheral blood cells of patients with B-cell non-Hodgkin's lymphoma

Evaluation of a newly developed HIV antigen test

Pediatric Human Immunodeficiency Virus Screening in an African District Hospital

Contact Info

Product

Resources

About