Standard G-, Q-, and R-banded ideograms of the domestic sheep &lt;i&gt;(Ovis aries):&lt;/i&gt; homology with cattle &lt;i&gt;(Bos taurus)&lt;/i&gt;. Report of the committee for the standardization of the sheep karyotype

The first centric-fusion (or Robertsonian) translocation in domestic sheep was reported by Bruere in 1969 (reviewed in BROAD et al. 1997). Since then, five different Robertsonian translocations have been reported in sheep in New Zealand (reviewed in BROAD et al. 1997). A sheep carrying one of these, designated the t, translocation (rob 5;8), died before passing it on (PEARCE et al. 1994), but the remaining four-designated t, (rob 6;24), t, (rob 9;10), t, (rob 7;25) and t, (rob 8;22) (ANSARI et al. 1993;PEARCE et al. 1994)-have been maintained in a nucleus breeding flock. For about the past two decades, sheep in this nucleus flock have been bred that are homozygous for the t,, t,, and t, translocations (2N = 48). In the 1999 breeding season, a male and female lamb has been generated carrying the t,, t,, t, and t, translocations in a homozygous state (2N=46). This is a unique occurrence. MATERIALS AND METHODSMetaphase chromosomes were prepared from whole blood cultures using standard techniques and banded by conventional Leishman-trypsin treatment. The numbering of the chromosomes followed the recommendations of the 1995 standard sheep karyotype (ANSARI et al. 1999). RESULTS AND DISCUSSIONA metaphase spread from the ram lamb that is homozygous for the four different Robertsonian translocations is shown in Fig. 1.Robertsonian translocations appear to be common in most of the members of the family Bovidae of over 100 species that are related through a series of centric fusions (reviewed by FRANKLIN 1997). For example, three such fusions are proposed to be involved in the Fig. 1. A partial GTG-banded metaphase spread from a ram lam showing four different homozygous Robertsonian translocations, t, (rob 6;24), t, (rob 9;10), t, (rob 7;25) and t, (rob 8;22).

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“…The numbering of the chromosomes followed the recommendations of the 1995 standard sheep karyotype (ANSARI et al 1999).…”

Section: Methodsmentioning

confidence: 99%

A Novel Occurrence of four Homozygous Robertsonian Translocations in Sheep (Ovis Aries)

Broad

McLean

Grimaldi³

et al. 2004

Hereditas

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“…18 For visualization of chromosome 13 we used either a chromosome specific paint probe for sheep (Ovis aries) chromosome 13, which is homologous to bovine chromosome 13 or a bovine chromosome 13 paint probe. 8,31,32 Chromosomes were sorted by fluorescence activated chromosome sorting (FACS) and their DNA was amplified using degenerate oligonucleotide-primed PCR (DOP-PCR) or a whole genome amplification kit (GE Healthcare). 31 An a-satellite probe for visualization of pericentromeric heterochromatin on bovine chromosomes was generated by DOP-PCR from bovine genomic DNA using sequence information for bovine satellites III and IV for the synthesis of appropriate primer pairs.…”

Section: Dna Probes and Labeling Proceduresmentioning

confidence: 99%

Positional changes of a pluripotency marker gene during structural reorganization of fibroblast nuclei in cloned early bovine embryos

Popken

Koehler

Brero

et al. 2014

Nucleus

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Cloned bovine preimplantation embryos were generated by somatic cell nuclear transfer (SCNT) of bovine fetal fibroblasts with a silent copy of the pluripotency reporter gene GOF, integrated at a single site of a chromosome 13. GOF combines the regulatory Oct4/Pou5f1 sequence with the coding sequence for EGFP. EGFP expression served as a marker for pluripotency gene activation and was consistently detected in preimplantation embryos with 9 and more cells. Threedimensional radial nuclear positions of GOF, its carrier chromosome territory and non-carrier homolog were measured in nuclei of fibroblasts, and of day 2 and day 4 embryos, carrying 2 to 9 and 15 to 22 cells, respectively. We tested, whether transcriptional activation was correlated with repositioning of GOF toward the nuclear interior either with a corresponding movement of its carrier chromosome territory 13 or via the formation of a giant chromatin loop. A significant shift of GOF away from the nuclear periphery was observed in day 2 embryos together with both carrier and non-carrier chromosome territories. At day 4, GOF, its carrier chromosome territory 13 and the non-carrier homolog had moved back toward the nuclear periphery. Similar movements of both chromosome territories ruled out a specific GOF effect. Pluripotency gene activation was preceded by a transient, radial shift of GOF toward the nuclear interior. The persistent co-localization of GOF with its carrier chromosome territory rules out the formation of a giant chromatin loop during GOF activation.

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“…The cattle, sheep, and goat karyotypes were arranged based on the G-banding standards developed by Ansari et al (1999) and Di Berardino et al (2001). Because there is no international standard for the G-banding pattern of Dama dama, the karyogram for this species was arranged based on chromosome morphology, chromosome size and G-band homology following the guidelines given by Rubini et al (1990).…”

Section: Laboratory Techniquesmentioning

confidence: 99%

“…This concerns chromosome banding patterns Di Meo, 1991, 1995;Ansari et al, 1999), nucleotide sequences (e.g., microsatellite sequences) (de Gortari et al, 1997) and groups of linked or syntenic genes that often have the same relationships even in taxonomically distant species (Slate et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Comparison of karyotypes after differential staining of chromosomes using GTG, RBA, RBG and QFQ techniques reveals conservation of chromosome banding patterns (Ansari et al, 1999;Słota et al, 2001). Identifi cation of homologous chromosomes or their fragments from different animal species is most often conducted within systematic units, providing further evidence that evolutionary relatedness is paralleled by karyotype similarity (Iannuzzi and Di Meo, 1995;Słota et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of GTG-banded karyotypes and microsatellite sequences in some species of the <i>Bovidae</i> and <i>Cervidae</i> families

Kozubska‐Sobocińska¹,

Ząbek²,

Słota³

et al. 2007

J. Anim. Feed Sci.

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Comparison of GTG-banded cattle, sheep, goat, and fallow deer chromosomes showed conformity of G-banding patterns for chromosomes of fallow deer. In Bovidae species, autosomes were shown that were involved in centric fusion, giving a metacentric pair in fallow deer.Twenty-two microsatellite markers were amplifi ed with the use of bovine specifi c primers in the investigated species. The results of cross-species amplifi cation showed that microsatellites BM143, CSSM016 and TGLA53 seem to be conservative across all investigated ruminant species. Sequencing confi rmed short tandem repeat motives of the TGLA53 marker among selected Bovidae and Cervidae species. About 60% of microsatellite loci were successfully amplifi ed with the use of bovine primers in sheep and red deer species, 50% in goat, 55% in western roe deer, and 36% in fallow deer. This suggests that, on the DNA level, the genomes of red deer and sheep are more closely related to the bovine genome than genomes of the other studied ruminants.

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Standard G-, Q-, and R-banded ideograms of the domestic sheep <i>(Ovis aries):</i> homology with cattle <i>(Bos taurus)</i>. Report of the committee for the standardization of the sheep karyotype

Cited by 20 publications

References 4 publications

A Novel Occurrence of four Homozygous Robertsonian Translocations in Sheep (Ovis Aries)

A Novel Occurrence of four Homozygous Robertsonian Translocations in Sheep (Ovis Aries)

Positional changes of a pluripotency marker gene during structural reorganization of fibroblast nuclei in cloned early bovine embryos

Comparison of GTG-banded karyotypes and microsatellite sequences in some species of the <i>Bovidae</i> and <i>Cervidae</i> families

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