Comparison of GTG-banded karyotypes and microsatellite sequences in some species of the <i>Bovidae</i> and <i>Cervidae</i> families

Abstract

Comparison of GTG-banded cattle, sheep, goat, and fallow deer chromosomes showed conformity of G-banding patterns for chromosomes of fallow deer. In Bovidae species, autosomes were shown that were involved in centric fusion, giving a metacentric pair in fallow deer.Twenty-two microsatellite markers were amplifi ed with the use of bovine specifi c primers in the investigated species. The results of cross-species amplifi cation showed that microsatellites BM143, CSSM016 and TGLA53 seem to be conservative across all investigated ruminant species. Sequencing confi rmed short tandem repeat motives of the TGLA53 marker among selected Bovidae and Cervidae species. About 60% of microsatellite loci were successfully amplifi ed with the use of bovine primers in sheep and red deer species, 50% in goat, 55% in western roe deer, and 36% in fallow deer. This suggests that, on the DNA level, the genomes of red deer and sheep are more closely related to the bovine genome than genomes of the other studied ruminants.