Stallion Sperm Integrity After Centrifugation to Reduce Seminal Plasma Concentration and Cool Storage for 4 days

Len, Jose; Beehan, D.; Eilts, B.E.; Ebrahimie, Esmaeil; Lyle, Sara K.

doi:10.1016/j.jevs.2019.102819

Cited by 6 publications

(7 citation statements)

References 21 publications

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“…One since of the main negative impacts on SP is the formation of reactive oxygen species (ROS; Monteiro et al, 2022). Quantities bigger than 10% SP in semen samples, in horses, cause negative effects on spermatozoa submitted cryopreservation and ROS participation is attributed in these cases (Len et al, 2020). But then, SP contains a complex mix of ions, enzymes, sugars, lipids, amino acids, proteins, and hormones (Neuhauser et al, 2019; Wood et al, 2016) that have an important role in physiological processes for sperm functions such as protection, nutrition, transport, regulation of the inflammatory response in the uterus and interaction with the oocyte (Portus et al, 2005; Töpfer‐Petersen et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Donkey semen cryopreservation: Alternatives with permeable, non‐permeable cryoprotectants and seminal plasma

Páez

Suarez

Betancur

2023

Reprod Domestic Animals

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Cryopreservation of semen is an important technique to preserve genetic material. Yet, pregnancy rates in jennies after artificial insemination with frozen–thawed donkey semen are poor. This condition has been attributed to the impact of permeable cryoprotectants, that could cause high post‐breeding endometritis. Removal of seminal plasma (SP) prior to semen freezing process is another contributing factor. SP is involved in a multitude of sperm functions and events preceding fertilization and has a mediating effect of sperm capacitation and postcoital uterine inflammatory response. The aim of this study was to evaluate different alternatives in donkey semen cryopreservation with permeable, non‐permeable cryoprotectants, BSA and SP. Thirty ejaculates from 10 donkeys were cryopreserved with different combinations of dimethylformamide (DMF, 5%), sucrose (SUC, 200 mM) and homologous SP (10%): DMF (T1), DMF/SP (T2), SUC/BSA (T3), SUC/BSA/SP (T4), DMF/SUC/BSA (T5), DMF/SUC/BSA/SP (T6), DMF/BSA (T7) and DMF/BSA/SP (T8). After thawing, sperm motility and kinetics were assessed by computerized semen analysis. Sperm vitality (SV) was evaluated by fluorescence microscopy, functional membrane integrity (FMI) by the HOST test, abnormal morphology by eosin‐nigrosin staining and sperm membrane stability by flow cytometry. For statistical analysis, sperm quality indexes (SQi) were obtained, general linear models were carried out and mean comparisons were made by the Tukey test. T1, T2, T5, T6, and T7 had higher and equivalent results for motility, most kinetic parameters and function membrane integrity. Cryopreservation of donkey semen without permeable cryoprotectant (T3 and T4) showed a reduction in motility, kinetics, SV, FMI and SQi. T5 showed a reduction in progressive motility, sperm velocities, IMF and SQi compared to other DMF treatments. T6 and T8 achieved higher SQi values compared to T1, but they were not different compared to T2 and T7. T1 had a smaller sperm population with low‐M540 compared to T3. It is concluded that the use of permeable cryoprotectant is essential to achieve higher post‐thaw quality of donkey semen. In addition, the combined use of BSA, SUC and/or PS may provide additional sperm protection compared to the individual use of DMF.

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Section: Discussionmentioning

confidence: 99%

Donkey semen cryopreservation: Alternatives with permeable, non‐permeable cryoprotectants and seminal plasma

Páez

Suarez

Betancur

2023

Reprod Domestic Animals

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“…Swim‐up and density gradient centrifugation are conventional sperm sorting techniques routinely used in artificial reproduction technologies (ART) because they meet many of these requirements. Both these techniques, however, include a centrifugation step which may cause sperm damage due to ROS production and the consequent increment of plasma membrane peroxidation (Len et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Microfluidic systems require low sample consumption. They allow for an easier manipulation of spermatozoa compared with traditional methods and without the drawbacks of centrifugation (Len et al, 2020). For these reasons, the use of microfluidic technology is of increasing interest within ART laboratories (Baldini et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…It should allow for isolation of sperm with high velocity, reduce reactive oxygen species (ROS) generation and morphological defects as well as the percentage of sperm with fragmented DNA (Asghar et al, 2014). Because centrifugation will increase the exposure of motile spermatozoa to ROS, this processing step should be avoided (Len et al, 2020). Swim-up and density gradient centrifugation are conventional sperm sorting techniques routinely used in artificial reproduction technologies (ART) because they meet many of these requirements.…”

mentioning

confidence: 99%

“…centrifugation step which may cause sperm damage due to ROS production and the consequent increment of plasma membrane peroxidation (Len et al, 2020).…”

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confidence: 99%

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Selection of frozen–thawed stallion semen by microfluidic technology

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Reprod Domestic Animals

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The ideal sperm sorting technique should be rapid, cheap and easy to use. It should allow for isolation of sperm with high velocity, reduce reactive oxygen species (ROS) generation and morphological defects as well as the percentage of sperm with fragmented DNA (Asghar et al., 2014). Because centrifugation will increase the exposure of motile spermatozoa to ROS, this processing step should be avoided (Len et al., 2020). Swim-up and density gradient centrifugation are conventional sperm sorting techniques routinely used in artificial reproduction technologies (ART) because they meet many of these requirements. Both these techniques, however, include a

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Use of Equine Sperm Cryopreservation Techniques as a Conservation Method of Donkey Germplasm

Monteiro

Cunha

Guerra

et al. 2022

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Stallion Sperm Integrity After Centrifugation to Reduce Seminal Plasma Concentration and Cool Storage for 4 days

Cited by 6 publications

References 21 publications

Donkey semen cryopreservation: Alternatives with permeable, non‐permeable cryoprotectants and seminal plasma

Donkey semen cryopreservation: Alternatives with permeable, non‐permeable cryoprotectants and seminal plasma

Selection of frozen–thawed stallion semen by microfluidic technology

Use of Equine Sperm Cryopreservation Techniques as a Conservation Method of Donkey Germplasm

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