Stalk Rot Diseases Impact Sweet Sorghum Biofuel Traits

Bandara, Y. M. A. Y.; Weerasooriya, Dilooshi K.; Tesso, Tesfaye; Little, Christopher R.

doi:10.1007/s12155-016-9775-6

Cited by 14 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At 32 days after inoculation (DAI) the stalk was split longitudinally, and the length of the resulting redto-purple discoloration resulting from wounding and pathogen ingress (defined as the lesion) was measured. This methodology has been commonly used in several other studies, including with plants of varying heights (Bandara et al 2017a;Funnell-Harris et al 2014;Tesso et al 2005). In addition to measurements of lesion lengths, plant heights after flowering and stalk diameters at site of inoculation (middle of second internode) were determined.…”

Section: Methodsmentioning

confidence: 99%

Response of Sorghum Enhanced in Monolignol Biosynthesis to Stalk Rot Pathogens

et al. 2019

View full text Add to dashboard Cite

To increase phenylpropanoid constituents and energy content in the versatile C4 grass sorghum (Sorghum bicolor [L.] Moench), sorghum genes for proteins related to monolignol biosynthesis were overexpressed: SbMyb60 (transcriptional activator), SbPAL (phenylalanine ammonia lyase), SbCCoAOMT (caffeoyl coenzyme A [CoA] 3-O-methyltransferase), Bmr2 (4-coumarate:CoA ligase), and SbC3H (coumaroyl shikimate 3-hydroxylase). Overexpression lines were evaluated for responses to stalk pathogens under greenhouse and field conditions. Greenhouse-grown plants were inoculated with Fusarium thapsinum (Fusarium stalk rot) and Macrophomina phaseolina (charcoal rot), which cause yield-reducing diseases. F. thapsinum-inoculated overexpression plants had mean lesion lengths not significantly different than wild-type, except for significantly smaller lesions on two of three SbMyb60 and one of two SbCCoAOMT lines. M. phaseolina-inoculated overexpression lines had lesions not significantly different from wild-type except one SbPAL line (of two lines studied) with mean lesion lengths significantly larger. Field-grown SbMyb60 and SbCCoAOMT overexpression plants were inoculated with F. thapsinum. Mean lesions of SbMyb60 lines were similar to wild-type, but one SbCCoAOMT had larger lesions, whereas the other line was not significantly different than wild-type. Because overexpression of SbMyb60, Bmr2, or SbC3H may not render sorghum more susceptible to stalk rots, these lines may provide sources for development of sorghum with increased phenylpropanoid concentrations.

show abstract

Section: Methodsmentioning

confidence: 99%

Response of Sorghum Enhanced in Monolignol Biosynthesis to Stalk Rot Pathogens

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Charcoal rot is a high priority fungal disease in sorghum [ Sorghum bicolor (L.) Moench], causing tremendous crop losses wherever sorghum is grown (Tarr, 1962, Tesso et al ., 2012). Recent studies have shown that charcoal rot can negatively affect the grain sorghum physical and chemical properties (Bandara et al, 2017a), yield parameters (Bandara et al, 2017b), and leaf greenness (Bandara et al, 2016), as well as the key biofuel traits of sweet sorghum (Bandara et al, 2017c). There are limited options available to control charcoal rot disease.…”

Section: Introductionmentioning

confidence: 99%

Host lipid alterations afterMacrophomina phaseolinainfection contribute to charcoal rot disease susceptibility in grain sorghum: Evidence from transcriptomic and lipidomic data

et al. 2019

Preprint

View full text Add to dashboard Cite

25• Lipids are involved in central metabolic processes and confer basic configuration to 26 cellular and subcellular membranes. Lipids also play a role in determining the outcome of 27 plant-pathogen interactions. Lipid based links that delineate either host resistance or 28 susceptibility against necrotrophic microorganisms are poorly investigated and described. 29 Macrophomina phaseolina (MP) is an important necrotrophic fungus which causes 30 diseases in over 500 plant species including charcoal rot in sorghum. 31 32 • We used RNA sequencing and automated direct infusion electrospray ionization-triple 33 quadrupole mass spectrometry (ESI-MS/MS) to quantitatively profile the transcriptomes 34 and lipidomes of a known charcoal rot resistant (SC599) and susceptible (Tx7000) 35 sorghum genotype in response to MP inoculation. 36 37 • We found that MP is capable of significantly decreasing the phosphatidylserine, 38 phytosterol, and ox-lipid contents in the susceptible genotype while significantly 39 increasing its stigmasterol:sitosterol and monogalactosyldiacylglycerol: 40 digalactosyldiacylglycerol ratios. None of the above was significantly affected in the 41 resistant genotype, except for the significantly increased ox-lipid content. 42 43 • Our transcriptome and functional lipidome findings suggested the lethal impacts of MP 44 inoculation on plastid-and cell-membrane integrity and the lipid based signaling 45

show abstract

“…With the recent interest in bioenergy feedstocks, sorghum has been recognized as a promising alternative for sustainable biofuel production (Kimber et al 2013). Recent studies have revealed the negative impacts of charcoal rot disease on grain sorghum physicochemical properties (Bandara et al 2017a), yield components (Bandara et al 2017b), and the staygreen trait (Bandara et al 2016), as well as the biofuel traits of sweet sorghum (Bandara et al 2018a; Bandara et al 2017c). As charcoal rot is a high priority fungal disease in sorghum [ Sorghum bicolor (L.) Moench], that causes crop losses where ever sorghum is grown (Tesso et al 2012), more research is needed to identify charcoal rot resistance/susceptibility mechanisms.…”

Section: Introductionmentioning

confidence: 99%

The necrotrophic fungus Macrophomina phaseolina induces oxidative stress-associated genes and related biochemical responses in charcoal rot susceptible sorghum genotypes

Bandara

Weerasooriya

Liu

et al. 2019

Preprint

View full text Add to dashboard Cite

Macrophomina phaseolina (MP) is a necrotrophic fungus that causes charcoal rot disease in sorghum [Sorghum bicolor (L.) Moench]. The host resistance and susceptibility mechanisms for this disease are poorly understood. Here, the transcriptional and biochemical aspects of the oxidative stress and antioxidant system of charcoal rot resistant and susceptible sorghum genotypes in response to MP inoculation were investigated. RNA sequencing revealed 96 differentially expressed genes between resistant (SC599) and susceptible (Tx7000) genotypes that are related to the host oxidative stress and antioxidant system. Follow-up functional experiments demonstrated MP’s ability to significantly increase reactive oxygen (ROS) and nitrogen species (RNS) content in the susceptible genotypes. This was confirmed by increased malondialdehyde content, an indicator of ROS/RNS-mediated lipid peroxidation. The presence of nitric oxide (NO) in stalk tissues of susceptible genotypes was confirmed using a NO-specific fluorescent probe (DAF-FM DA) and visualized by confocal microscopy. Inoculation significantly increased peroxidase activity in susceptible genotypes while catalase activity was significantly higher in MP-inoculated resistant genotypes. MP inoculation significantly reduced superoxide dismutase activity in all genotypes. These findings suggested MP’s ability to promote a host-derived oxidative stress response in susceptible sorghum genotypes, which contributes to induced cell death-associated disease susceptibility to this necrotrophic phytopathogen.

show abstract

Stalk Rot Diseases Impact Sweet Sorghum Biofuel Traits

Cited by 14 publications

References 27 publications

Response of Sorghum Enhanced in Monolignol Biosynthesis to Stalk Rot Pathogens

Response of Sorghum Enhanced in Monolignol Biosynthesis to Stalk Rot Pathogens

Host lipid alterations afterMacrophomina phaseolinainfection contribute to charcoal rot disease susceptibility in grain sorghum: Evidence from transcriptomic and lipidomic data

The necrotrophic fungus Macrophomina phaseolina induces oxidative stress-associated genes and related biochemical responses in charcoal rot susceptible sorghum genotypes

Contact Info

Product

Resources

About