Host lipid alterations afterMacrophomina phaseolinainfection contribute to charcoal rot disease susceptibility in grain sorghum: Evidence from transcriptomic and lipidomic data

Abstract

25• Lipids are involved in central metabolic processes and confer basic configuration to 26 cellular and subcellular membranes. Lipids also play a role in determining the outcome of 27 plant-pathogen interactions. Lipid based links that delineate either host resistance or 28 susceptibility against necrotrophic microorganisms are poorly investigated and described. 29 Macrophomina phaseolina (MP) is an important necrotrophic fungus which causes 30 diseases in over 500 plant species including charcoal rot in sorghum. 31 32 • We used RNA sequencing and automated direct infusion electrospray ionization-triple 33 quadrupole mass spectrometry (ESI-MS/MS) to quantitatively profile the transcriptomes 34 and lipidomes of a known charcoal rot resistant (SC599) and susceptible (Tx7000) 35 sorghum genotype in response to MP inoculation. 36 37 • We found that MP is capable of significantly decreasing the phosphatidylserine, 38 phytosterol, and ox-lipid contents in the susceptible genotype while significantly 39 increasing its stigmasterol:sitosterol and monogalactosyldiacylglycerol: 40 digalactosyldiacylglycerol ratios. None of the above was significantly affected in the 41 resistant genotype, except for the significantly increased ox-lipid content. 42 43 • Our transcriptome and functional lipidome findings suggested the lethal impacts of MP 44 inoculation on plastid-and cell-membrane integrity and the lipid based signaling 45

Plant lipid metabolism in susceptible and tolerant soybean (Glycine max) cultivars in response to Phytophthora sojae colonization and infection

“…Plants can also adapt to environmental stresses by regulating biochemical, physiological, and molecular properties of their cellular membrane (Adigun et al 2020). Several studies have demonstrated the roles of lipids in plant pathology as part of a complex internal defense mechanism in the fight against infections caused by various pathogens (Adigun et al 2020, Bandara et al 2019, Lim et al 2017, Raffaele et al 2009). Lipid remodeling is a defence mechanism adopted by plants to counteract pathogen attack (Goufo and Cortez 2020).…”