Staining the Ground Section of the Tooth Using an Innovative Plant Stain Found in the Pravara Region, India

Vikhe, Deepak M; Thete, Sanjay G; Mastud, Chaitra; Mantri, Tejashree; Mhaske, Prasad N; Mastud, Santoshkumar P; Madanshetty, Pallavi

doi:10.5005/jp-journals-10024-2904

Cited by 1 publication

(4 citation statements)

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“…Sectioning should be done perpendicular to the long axis of a target component/structure ( e.g. , a metal implant or a tooth root), or the target object can be distorted in the resulting section ( Vikhe et al, 2020 ; Parfitt et al, 1987 ).…”

Section: Discussionmentioning

confidence: 99%

“…This is likely because structures with limited mineralization and high organic matter content have a greater affinity for pigment compared with highly mineralized areas, making these less-mineralized structures more visible and easily identifiable when stained with pigment-containing rosin. Vikhe et al (2020) used the Pravara (a plant stain) staining process for analyzing the ground sections of teeth, with the expectation that hard tissue visibility would be improved with this process. However, the authors concluded that the grade of stained sections and the maintenance time of the staining effect should be compared in subsequent studies as their study did not compare these variables when considering this process ( Vikhe et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“… Vikhe et al (2020) used the Pravara (a plant stain) staining process for analyzing the ground sections of teeth, with the expectation that hard tissue visibility would be improved with this process. However, the authors concluded that the grade of stained sections and the maintenance time of the staining effect should be compared in subsequent studies as their study did not compare these variables when considering this process ( Vikhe et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…This process involves the preparation of thin sections through grinding or the use of a hard tissue microtome ( Donath, 1995 ; Suvarna, Layton & Bancroft, 2013 ). Vikhe et al (2020) reported that tooth hard tissue could be clearly examined after using borite as a grinding aid followed by the Pravara staining process. These conventional methods of preparing ground sections are time-consuming, laborious, involve complicated procedures, and result in sections with nonuniform thickness.…”

Section: Introductionmentioning

confidence: 99%

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An improved method for preparing stained ground teeth sections

Qin

Zhou

2023

PeerJ

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Objective In oral histopathology teaching and research, there is a need for high-quality undemineralized tooth sections that are easy to handle, have controlled thickness, allow the observation of intact microstructures, and can be preserved for long periods of time. Methods Teeth were collected under non-demineralizing conditions. Tooth sections (15–25 µm) were prepared using a diamond knife, then randomly divided into three groups: (1) stained with rosin, (2) stained with hematoxylin and eosin, or (3) not stained. The prepared tooth sections were evaluated by microscopy for clarity and microstructure visibility. Results The use of a diamond knife in the sectioning and grinding process yielded high-quality ground sections of teeth. Rosin-stained ground sections allowed better identification of microstructures within the teeth, compared with unstained or hematoxylin and eosin-stained ground sections. Conclusion The best results were obtained in the ground sections of teeth that were stained with rosin. Ground sections of teeth prepared using this staining method could be useful in oral histopathology teaching and research.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%