Staining efficacy assessment of a differential routine and special stains for pathological stromal calcifications in maxillofacial lesions

Shefali,; Sethi, Aashka; Tandon, Ankita; Shetty, Devi Charan; Juneja, Saurabh

doi:10.1080/01478885.2020.1739192

Cited by 6 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is ideal for investigating the static parameters of bone formation, such as osteoblast index, osteoid width, osteoid surface, and osteoid volume. After staining with methylene blue-acid fuchsin, the osteoblast nuclei are stained dark blue, the cell-matrix is stained light blue, the osteoid is purple-gray, the new bone is dark red, and the original mineralized bone tissue is red [23,24]. In the G1 group (figure 11), the thickness of neonatal bone tissue around the prosthesis was significantly larger than that in other groups.…”

Section: Methylene Blue-acid Fuchsin Staining Of Femur Sectionmentioning

confidence: 99%

“…Von Kossa staining is a commonly used method in bone histological observation, and we can identify different tissues by different color and morphology. After staining with Von Kossa, the mature bone is stained dark brown, and the osteoid generated during the bone formation is orange [24,27]. As the osteoid matures and mineralizes, the orange of osteoid gradually deepens, so it can be considered that the color depth indicates the level of osteoid mineralization.…”

Section: Von Kossa Histological Staining Of Femur Sectionmentioning

confidence: 99%

See 1 more Smart Citation

Research on the inhibition for aseptic loosening of artificial joints by Sr-doped calcium polyphosphate (SCPP) in vivo

Xu¹,

Li²,

Cheng³

et al. 2021

Biomed. Mater.

View full text Add to dashboard Cite

Aseptic loosening of artificial joints is the most common complication after artificial joint replacement. Finding the solution to tackle aseptic loosening of artificial joints is a focus in bone and joint surgery research field. In vitro studies of Sr-doped calcium polyphosphate (SCPP) have found by our team that it could promote osteoblast proliferation and inhibit osteoclast activity, and it has a potential inhibitory effect on aseptic loosening by suppressing the expression of receptor activator of nuclear factor-κ B ligand and improving the expression of OPG. The present study aims to confirm the conclusion in vitro by the mean of animal experiment. The Ti rod prosthesis coated with SCPP, calcium polyphosphate (CPP), and Ultra-high molecular weight polyethylene (UHMWPE were implanted in the femur (the internal surface of bone tunnel was also coated with SCPP, CPP and UHMWPE respectively). Radiography (x-rays, micro-CT), histochemistry (Hematoxylin-eosin staining (HE), methylene blue-acid fuchsin, Von Kossa histological staining), molecular biology (alkaline phosphatase and TRAP5b factors, Mir21-5p and Mir 26a-5p) were performed to analyzed the effects of SCPP within 20 weeks. The Radiography results showed that osteolysis with various severity occurred in all groups, and SCPP group had the mildest osteolysis. Histochemistry results showed that arthritis was milder in SCPP and CPP groups, while the bone formation in SCPP group was most significant. Its bone reconstruction effect was the best as well. The Molecular biology results showed that the bone reconstruction was out-sync in each group. Compared with other groups, the bone resorption occurred at the latest and the bone resorption time was the shortest in experimental animals of SCPP group. All results indicated that SCPP could promote osteoblast activity and bone reconstruction, improve the integration of bone interface between prosthesis and base bone, reduce osteoclast activity and shorten the osteoclast action time at the implantation site in vivo. Thus, it could postpone or alleviate the occurrence and development of aseptic loosening in vivo. Therefore, SCPP could be a promising material for the construction of artificial joints with the ability to resist aseptic loosening.

show abstract

Section: Methylene Blue-acid Fuchsin Staining Of Femur Sectionmentioning

confidence: 99%

Section: Von Kossa Histological Staining Of Femur Sectionmentioning

confidence: 99%

Research on the inhibition for aseptic loosening of artificial joints by Sr-doped calcium polyphosphate (SCPP) in vivo

Xu¹,

Li²,

Cheng³

et al. 2021

Biomed. Mater.

View full text Add to dashboard Cite

show abstract

“…As a special staining technique, MT stain has been routinely used, although scanty bone tissue might be lost in the background of collagen [30]. It has been reported that MB/AF stain is a better way to differentiate bone and stromal tissues [31]. In this study, bone sections were stained with MB/AF.…”

Section: Discussionmentioning

confidence: 99%

A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)

Kang

Liu

Wang

et al. 2021

BMC Musculoskelet Disord

View full text Add to dashboard Cite

Background Rats have been widely used as experimental animals when performing fundamental research because they are economical, rapidly reproducing, and heal quickly. While the rat interbody fusion model has been applied in basic studies, existing rat models generally have shortcomings, such as insufficiently simulating clinical surgery. The purpose of this study was to develop a novel rat model of interbody fusion which more closely represents clinical surgery. Methods The internal fixation was designed based on physical measurements of the rats’ lumbar spine. Then, ten rats divided into two groups (A and B) underwent anterior lumbar corpectomy and fusion of the L5 vertebrae. Groups A and B were sacrificed four and 8 weeks post-surgery, respectively. Micro-CT and histological examination were used to evaluate the model. Fusion rate, bone volume fraction (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th), and the area ratio of newly formed bone (NB) were calculated for quantitative analysis. Results Based on the L5 body dimensions of individual rats, 3D-printed titanium cage of the appropriate size were printed. The operations were successfully completed in all ten rats, and X-ray confirmed that internal fixation was good without migration. Micro-CT suggested that fusion rates in group B (100%) were greater than group A (40%, P < 0.05). The BV/TV (B: 42.20 ± 10.50 vs. A: 29.02 ± 3.25, P < 0.05) and Tb.N (B: 4.66 ± 1.23 vs. A: 1.97 ± 0.40, P < 0.05) were greater in group B than A, and the Tb.Th in group B was lower than group A (B: 0.10 ± 0.04 vs. A: 0.15 ± 0.02, P < 0.05). Histomorphometry results demonstrated that the area ratio of NB in group B were greater than group A (B: 35.72 ± 12.80 vs. A: 12.36 ± 16.93, P < 0.05). Conclusion A rat interbody fusion model based on anterior lumbar corpectomy and fusion has successfully been constructed and verified. It could provide a new choice for fundamental research using animal models of spinal fusion.

show abstract

“…Samples were then processed to obtain paraffin blocks. Sections (6 µm in thickness) were stained with hematoxylin and Eosin (H & E) and Masson's trichrome for light microscopy examination [27]. To measure the mean area percent of collagen fibers in Masson's trichrome stained sections (X400) in the cardiac tissue, ten scattered non-overlapping fields were chosen.…”

Section: Histological Examinationmentioning

confidence: 99%

Machine Learning-Based ECG Signal Classification for Enhanced Early Detection of Doxorubicin-Induced Cardiotoxicity in Rats

Mohammed,

H.Hanafy,

abdo

et al. 2024

Preprint

View full text Add to dashboard Cite

Cardiotoxicity, which leads to irreversible myocardial damage, is a major adverse effect associated with chemotherapy. Electrocardiogram (ECG) is an inexpensive, rapid, and simple tool that may provide valuable diagnostic information pertinent to cardiotoxicity. An automatic interpretation and classification of the ECG signals by machine learning algorithms is considered superior to human interpretation of the ECG which may not be able to early detect subtle alterations in the ECG and vary according to the experience of the specialist. The present work aimed at using different machine learning algorithms to classify ECG signals recorded from doxorubicin-injected rats. Rats were divided into four groups and each group was intraperitoneally injected with different cumulative doses of doxorubicin (0, 6, 12, and 18 mg/kg). ECG signal classification depended on multiple features that were extracted from the recorded signals under different conditions. K nearest neighbors’ algorithm achieved higher classification accuracy (99.83 %) than random forest (99.56 %), decision tree (99.54 %), artificial neural network (99.50 %), and support vector machine (99.38 %). Furthermore, the dose-dependent cardiotoxicity was validated via a histopathological examination of the left ventricle that indicated significant pathological alterations in the cardiac tissue. The present findings emphasized the potential of the machine learning-based enhanced detection of cardiotoxicity and validated the dose-dependent toxicity of doxorubicin in the cardiac left ventricle. This approach might be applicable clinically to avoid cardiotoxicity in chemotherapy-treated patients.

show abstract

Staining efficacy assessment of a differential routine and special stains for pathological stromal calcifications in maxillofacial lesions

Cited by 6 publications

References 5 publications

Research on the inhibition for aseptic loosening of artificial joints by Sr-doped calcium polyphosphate (SCPP) in vivo

Research on the inhibition for aseptic loosening of artificial joints by Sr-doped calcium polyphosphate (SCPP) in vivo

A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)

Machine Learning-Based ECG Signal Classification for Enhanced Early Detection of Doxorubicin-Induced Cardiotoxicity in Rats

Contact Info

Product

Resources

About