Stable Low-Dose Oxygen Release Using H2O2/Perfluoropentane Phase-Change Nanoparticles with Low-Intensity Focused Ultrasound for Coronary Thrombolysis

Jiang, Nan; Hu, Bo; Cao, Sheng; Gao, Shunji; Cao, Qingqiong; Chen, Jinling; Zhou, Qing; Guo, Ruiqiang

doi:10.1016/j.ultrasmedbio.2020.06.004

Cited by 9 publications

(5 citation statements)

References 24 publications

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“… 37 Based on our experimental results, under LIFU irradiation at 2 W/cm 2 for 10 min, the temperature reached 39–40°C, which stimulated membrane and cytoplasmic enzyme activities, leading to active ion transport in macrophages and thus triggering the release of NPs. 48 This temperature and the radiation intensity of LIFU will not cause harm to the human body in a short time, 16 , 49 indicating that this method is promising for clinical application. Moreover, most of the MTNPs remained alive at 2 W/cm 2 for 10 min, and the temperature did not damage the surrounding tissues, indicating the relative safety of LIFU irradiation.…”

Section: Resultsmentioning

confidence: 99%

Magnet-Guided Bionic System with LIFU Responsiveness and Natural Thrombus Tropism for Enhanced Thrombus-Targeting Ability

Fang¹,

Liu²,

Hou³

et al. 2022

IJN

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Background Arterial thrombosis is a serious threat to human health. Recently, many thrombus-targeted nanoparticles (NPs) have been constructed for detecting thrombi or monitoring thrombolysis, but their thrombus-targeting performance is limited. Considering this drawback, we designed a specific bionic system with enhanced thrombus-targeting ability. Materials and Methods In the bionic system, gelatin was chosen as a carrier, and Fe 3 O 4 served as a magnetic navigation medium and a magnetic resonance (MR) imaging agent. The CREKA peptide, which targets fibrin, was conjugated to the surface of gelatin to prepare targeted NPs (TNPs), which were then engulfed by macrophages to construct the bionic system. At the targeted site, the bionic system released its interior TNPs under low-intensity focused ultrasound (LIFU) irradiation. Moreover, the targeting performance was further improved by the conjugated CREKA peptide. Results In this study, we successfully constructed a bionic system and demonstrated its targeting ability in vitro and in vivo. The results indicated that most TNPs were released from macrophages under LIFU irradiation at 2 W/cm 2 for 10 min in vitro. Additionally, the enhanced thrombus-targeting ability, based on the natural tropism of macrophages toward inflammatory thrombi, magnetic navigation and the CREKA peptide, was verified ex vivo and in vivo. Moreover, compared with the bionic system group, the group treated with TNPs had significantly decreased liver and spleen signals in MR images and significantly enhanced liver and spleen signals in fluorescence images, indicating that the bionic system is less likely to be cleared by the reticuloendothelial system (RES) than TNPs, which may promote the accumulation of the bionic system at the site of the thrombus. Conclusion These results suggest that the magnet-guided bionic system with LIFU responsiveness is an excellent candidate for targeting thrombi and holds promise as an innovative drug delivery system for thrombolytic therapy.

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Section: Resultsmentioning

confidence: 99%

Magnet-Guided Bionic System with LIFU Responsiveness and Natural Thrombus Tropism for Enhanced Thrombus-Targeting Ability

Fang¹,

Liu²,

Hou³

et al. 2022

IJN

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“…Nanodroplets are driven into the thrombus by the mechanical injection generated by the first cavitation. Then when the f 2 ultrasound (responding to phase change and forming microbubbles [ 34 ] ) is turned on, the cavitation of nanodroplets within the thrombus occurs and droplets expand, which forms pores inside the thrombus, to make the thrombus porous, accelerate the drug diffusion and improve the efficiency of thrombolysis. See the Experimental Section and Figure S7 in the Supporting Information for the parameters of the ultrasound.…”

Section: Resultsmentioning

confidence: 99%

“…Cavitation can be occurred when pressure output and temperature reach the threshold. [ 16,31 ] However, the pressure output and temperature are set under specific values to limit bioeffects risk. The mechanical index (MI) of the ultrasound should be controlled below 1.0, which is within the safe range according to the international electrical commission (IEC) standard 61157‐2007 and temperature also needs to be kept in a safe range.…”

Section: Introductionmentioning

confidence: 99%

Nanodroplet‐Coated Microbubbles Used in Sonothrombolysis with Two‐Step Cavitation Strategy

Pan

et al. 2022

Adv Healthcare Materials

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Thrombosis is a major cause of morbidity and mortality and sonothrombolysis is a promising method for its treatment. However, the slow diffusion of the thrombolytic agents into the thrombus results in slow recanalization. Here, nanodroplet‐coated microbubbles (NCMBs) are designed and fabricated and a two‐step cavitation strategy is used to accelerate the thrombolysis. The first cavitation of the NCMBs, cavitation and collapse of the microbubbles induced by low frequency ultrasound, drives the nanodroplets on the shell into the thrombus, while the second cavitation, the phase‐change and volume expansion of drug‐loaded nanodroplets triggered by high frequency ultrasound, loosens the thrombus by the sono‐porosity effect. This two‐step cavitation of the NCMBs is verified using a fibrin agarose model, where a rapid diffusion of the thrombolytic agents is observed. Furthermore, the NCMBs reach much higher thrombolysis efficiency in both in vitro and proof‐of‐concept experiments performed with living mice. The nanodroplet‐coated microbubbles are a promising diffusion medicines carrier for efficient drug delivery.

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“…Additionally, ultrasound-irradiated phase-change nanoparticles have great potential for vascular thrombolysis. Jiang et al (2020) designed an extracorporeal artificial circulation system to simulate thromboembolism in vascular circulation in vitro . H2O2/PFP phase transition nanoparticles could produce sustained ultrasound-targeted microbubble destruction (UTMD) with a durable cavitation effect and high thrombolytic efficiency.…”

Section: Intravascular Targeting Nanoparticlesmentioning

confidence: 99%

Advances in nanomaterial-based targeted drug delivery systems

Cheng

Xie

Sun

2023

Front. Bioeng. Biotechnol.

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Nanomaterial-based drug delivery systems (NBDDS) are widely used to improve the safety and therapeutic efficacy of encapsulated drugs due to their unique physicochemical and biological properties. By combining therapeutic drugs with nanoparticles using rational targeting pathways, nano-targeted delivery systems were created to overcome the main drawbacks of conventional drug treatment, including insufficient stability and solubility, lack of transmembrane transport, short circulation time, and undesirable toxic effects. Herein, we reviewed the recent developments in different targeting design strategies and therapeutic approaches employing various nanomaterial-based systems. We also discussed the challenges and perspectives of smart systems in precisely targeting different intravascular and extravascular diseases.

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Stable Low-Dose Oxygen Release Using H2O2/Perfluoropentane Phase-Change Nanoparticles with Low-Intensity Focused Ultrasound for Coronary Thrombolysis

Cited by 9 publications

References 24 publications

Magnet-Guided Bionic System with LIFU Responsiveness and Natural Thrombus Tropism for Enhanced Thrombus-Targeting Ability

Magnet-Guided Bionic System with LIFU Responsiveness and Natural Thrombus Tropism for Enhanced Thrombus-Targeting Ability

Nanodroplet‐Coated Microbubbles Used in Sonothrombolysis with Two‐Step Cavitation Strategy

Advances in nanomaterial-based targeted drug delivery systems

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