Stable lentiviral transformation of CHO cells for the expression of the hemagglutinin H5 of avian influenza virus in suspension culture

Abstract

HighlightsWe obtained clones that stably expressed hemagglutinin in CHO cells by lentiviral vector transduction.The growth kinetics and the stable production of the hemagglutinin protein were demonstrated in the clone with the highest expression level.Hemagglutinin purification was carried out by immunoaffinity chromatography with a high degree of purity.ELISA assays using the hemagglutinin protein direct from the supernatant or in its purified form showed similar antibody titers.

Cell growth on 3D microstructured surfaces

“…It is known that cell growth is usually anchorage dependent and requires attachment to a solid surface [2][3][4], and cell adhesion to biomaterials with a suitable surface is fundamental to many biotechnological processes [5,6]. Numerous human and veterinary use pharmaceuticals, such as human tissue plasmid activator [7,8] and viral vaccines (hepatitis [9], rabies [10], influenza [11], polio, rotavirus, food engineering [12] and mouth disease [12,13]) are produced by anchorage dependent cells. Geometrical and mechanical properties of the cell microenvironment have great impact on cell morphogenesis and function, and can interfere with cell cytoskeleton architecture, polarity, migration, division, growth and differentiation.…”