Cell growth on 3D microstructured surfaces

Araujo, W. W. R.; Teixeira, F. S.; Silva, Glenda Nicioli da; Salvadori, Daisy Maria Fávero; Salvadori, M. C.; Brown, Ian

doi:10.1016/j.msec.2016.03.026

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…Furthermore, in the scaffolds with 75 μm wide features, myoblast cells adhered on the peaks of the microstructures and seem to spread beyond the edges of the lines evidencing interconnections, while in the structures 150 μm wide they are totally separated by the valleys and mostly adhered at the peaks of the microstructures. These results may indicate a preferential and thus larger adhesion of myoblast cells in the presence of minor spaces so they can be closer, than microstructures with higher height and wider valleys that hinder or even prevent cells communication …”

Section: Results and Discussionmentioning

confidence: 93%

“…The results demonstrated that the topography dimensions influence substantially cell behavior. SU-8 scaffolds with surfaces composed by hexagons with diameter in the range of the micrometers were fabricated to study the impact of the size in Chinese hamster Ovary (CHO) cell growth . Once again, cell growth density proved to depend directly in the microcavity radius, providing adequate distance for cells communication.…”

Section: Introductionmentioning

confidence: 99%

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Tuning Myoblast and Preosteoblast Cell Adhesion Site, Orientation, and Elongation through Electroactive Micropatterned Scaffolds

Marques‐Almeida¹,

Cardoso

Ribeiro³

et al. 2019

ACS Appl. Bio Mater.

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Electroactive polymers are being increasingly used in tissue engineering applications. Together with the electromechanical clues, morphological ones have been demonstrated to determine cell proliferation and differentiation. This work reports on the micropatterning of poly(vinylidene fluoride-co-trifluoroethylene), P(VDF-TrFE) scaffolds, and their interaction with myoblast and preosteoblasts cell lines, selected based on their different functional morphology. The scaffolds were obtained by soft lithography and obtained in the form of arrays of lines, intermittent lines, hexagons, linear zigzags, and curved zigzags with dimensions of 25, 75, and 150 μm. Moreover, the scaffolds were tested in cell adhesion assays of myoblasts and preosteoblasts cell lines. The results show that more linear surface topographies and dense morphology have a large potential in the regeneration of musculoskeletal tissue, while nonpatterned scaffolds or more anisotropic surface microstructures present largest potential to promote the growth and regeneration of bone tissue. In this way, cell adhesion site, orientation, and elongation can be controlled by choosing properly the topography and morphology of the scaffolds, indicating their suitability and potential for further proliferation and differentiation assays.

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Section: Results and Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Tuning Myoblast and Preosteoblast Cell Adhesion Site, Orientation, and Elongation through Electroactive Micropatterned Scaffolds

Marques‐Almeida¹,

Cardoso

Ribeiro³

et al. 2019

ACS Appl. Bio Mater.

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“…Only a few studies work with microstructured inorganic or organic surfaces for cells growth. Araujo et al [64] reported on templates consisting of SU-8, an epoxy-based material hexagonal with a periodic pattern of microcavities with a size ranging from 12 to 560 µm and its influence on cell growth of Chinese hamster ovary (CHO) cells. The honeycomb-like structures found the highest cell density of single, separated cells for the 80 µm cavities.…”

Section: Discussionmentioning

confidence: 99%

Complex Tumor Spheroid Formation and One-Step Cancer-Associated Fibroblasts Purification from Hepatocellular Carcinoma Tissue Promoted by Inorganic Surface Topography

et al. 2021

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In vitro cell models play important roles as testbeds for toxicity studies, drug development, or as replacements in animal experiments. In particular, complex tumor models such as hepatocellular carcinoma (HCC) are needed to predict drug efficacy and facilitate translation into clinical practice. In this work, topographical features of amorphous silicon dioxide (SiO2) are fabricated and tested for cell culture of primary HCC cells and cell lines. The topographies vary from pyramids to octahedrons to structures named fractals, with increased hierarchy and organized in periodic arrays (square or Hexagonal). The pyramids were found to promote complex 2D/3D tissue formation from primary HCC cells. It was found that the 2D layer was mainly composed of cancer-associated fibroblasts (CAFs), while the 3D spheroids were composed of tumor cells enwrapped by a CAF layer. Compared with conventional protocols for 3D cultures, this novel approach mimics the 2D/3D complexity of the original tumor by invading CAFs and a microtumor. Topographies such as octahedrons and fractals exclude tumor cells and allow one-step isolation of CAFs even directly from tumor tissue of patients as the CAFs migrate into the structured substrate. Cell lines form spheroids within a short time. The presented inorganic topographical surfaces stimulate complex spheroid formation while avoiding additional biological scaffolds and allowing direct visualization on the substrate.

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“…Interactions with the extracellular matrix (ECM), furthermore, influence cell behaviour and have become increasingly important for the understanding of cell development [2, 3]. Alongside the impact of physicochemical matrix composition, various studies have demonstrated that topography alone can significantly modulate cell function [4–7].…”

Section: Introductionmentioning

confidence: 99%

Cell Morphology on Poly(methyl methacrylate) Microstructures as Function of Surface Energy

Katschnig

Maroh

Andraschek

et al. 2019

International Journal of Biomaterials

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Whilst the significance of substrate topography as a regulator of cell function is well established, a systematic analysis of the principles underlying this is still unavailable. Here we evaluate the hypothesis that surface energy plays a decisive role in substrate-mediated modulation of cell phenotype by evaluation of cell behaviour on synthetic microstructures exhibiting pronounced differences in surface energy. These microstructures, specifically cubes and walls, were fabricated from a biocompatible base polymer, poly(methyl methacrylate), by variotherm injection molding. The dimensions of the cubes were 1 μm x 1 μm x 1 μm (height x width x length) with a periodicity of 1:1 and 1:5 and the dimensions of the walls 1 μm x 1 μm x 15 mm (height x width x length) with a periodicity of 1:1 and 1:5. Mold inserts were made by lithography and electroplating. The surface energy of the resultant microstructures was determined by static contact angle measurements. Light scanning microscopy of the morphology of NT2/D1 and MC3T3-E1 preosteoblast cells cultured on structured PMMA samples in both cases revealed a profound surface energy dependence. “Walls” appeared to promote significant cell elongation, whilst a lack of cell adhesion was observed on “cubes” with the lowest periodicity. Contact angle measurements on walls revealed enhanced surface energy anisotropy (55 mN/m max., 10 mN/m min.) causing a lengthwise spreading of the test liquid droplet, similar to cell elongation. Surface energy measurements for cubes revealed increased isotropic hydrophobicity (87° max., H2O). A critical water contact angle of ≤ 80° appears to be necessary for adequate cell adhesion. A “switch” for cell adhesion and subsequently cell growth could therefore be applied by, for example, adjusting the periodicity of hydrophobic structures. In summary cell elongation on walls and a critical surface energy level for cell adhesion could be produced for NT2/D1 and MC3T3-E1 cells by symmetrical and asymmetrical energy barrier levels. We, furthermore, propose a water-drop model providing a common physicochemical cause regarding similar cell/droplet geometries and cell adhesion on the investigated microstructures.

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Cell growth on 3D microstructured surfaces

Cited by 5 publications

References 34 publications

Tuning Myoblast and Preosteoblast Cell Adhesion Site, Orientation, and Elongation through Electroactive Micropatterned Scaffolds

Tuning Myoblast and Preosteoblast Cell Adhesion Site, Orientation, and Elongation through Electroactive Micropatterned Scaffolds

Complex Tumor Spheroid Formation and One-Step Cancer-Associated Fibroblasts Purification from Hepatocellular Carcinoma Tissue Promoted by Inorganic Surface Topography

Cell Morphology on Poly(methyl methacrylate) Microstructures as Function of Surface Energy

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