Stable isotope composition of biogas allows early warning of complete process failure as a result of ammonia inhibition in anaerobic digesters

Z, Lv; Hu, Meng Chun; Harms, Hauke; Richnow, Hans H.; Liebetrau, Jan; Nikolausz, Marcell

doi:10.1016/j.biortech.2014.06.029

Cited by 70 publications

(46 citation statements)

References 35 publications

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“…2A). The increase in VFA concentrations was consistent with an overall biogas and specific methane production rate decrease (Niu et al, 2013b;Lv et al, 2014). Further treatments resulted in more efficient VFA destruction with their better involvement in methanogenesis (days 47-58).…”

Section: Process Stability and Biogas Productionsupporting

confidence: 73%

“…However, even some ammonium compounds are beneficial for microbial growth, free ammonia above critical concentrations has been considered as the cause of inhibition of microorganisms during anaerobic digestion of nitrogen-rich substrates (Abouelenien et al, 2010). Especially ammonia is the most significant inhibitor of the last stage, methanogenesis, and therefore high amounts of NH 3 can decrease overall specific methane production (Niu et al, 2013b;Lv et al, 2014). Different environmental parameters, mostly rising temperature and pH, impact on balance of NH 3 /NH 4 + (total ammonia nitrogen, TAN) and can lead to the increased effect of toxicity of ammonia on anaerobic microorganisms (Garcia and Angenent, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Impact of the substrate loading regime and phosphoric acid supplementation on performance of biogas reactors and microbial community dynamics during anaerobic digestion of chicken wastes

Belostotskiy

Ziganshina

Siniagina

et al. 2015

Bioresource Technology

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Section: Process Stability and Biogas Productionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Impact of the substrate loading regime and phosphoric acid supplementation on performance of biogas reactors and microbial community dynamics during anaerobic digestion of chicken wastes

Belostotskiy

Ziganshina

Siniagina

et al. 2015

Bioresource Technology

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“…The hydrogen sulphide was not measured regularly but spot measurements gave an average pre-treatment H2S reading of > 200 ppm (out of range of the sensor used) and an average post-treatment reading of 178 ppm. To understand the reason for the downward trend in methane composition, further analysis would be required; it is possible that the change in the feedstock composition led to a natural reduction in the biogas composition, but it could also be an early sign of process instability (Lv et al, 2014); this is discussed further in section 3.5. The decrease in biogas production volume was not caused by a reduction in the OLR (which remained fairly constant from around day 235 onwards, at around 2.2 kg m -3 day -1 , shown in Figure 6(a)) but a reduction in the VS concentration of the mixed biomass in the pre-digester tank, which decreased from around day 297 onwards, as shown in Figure 4.…”

Section: Biogas Productionmentioning

confidence: 99%

Assessment of micro-scale anaerobic digestion for management of urban organic waste: A case study in London, UK

et al. 2017

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This paper describes the analysis of an AD plant that is novel in that it is located in an urban environment, built on a micro-scale, fed on food and catering waste, and operates as a purposeful system. The plant was built in 2013 and continues to operate to date, processing urban food waste and generating biogas for use in a community café. The plant was monitored for a period of 319 days during 2014, during which the operational parameters, biological stability and energy requirements of the plant were assessed. The plant processed 4574 kg of food waste during this time, producing 1008 m 3 of biogas at average 60.6 % methane. The results showed that the plant was capable of stable operation despite large fluctuations in the rate and type of feed. Another innovative aspect of the plant was that it was equipped with a pre-digester tank and automated feeding, which reduced the effect of 2 feedstock variations on the digestion process. Towards the end of the testing period, a rise in the concentration of volatile fatty acids and ammonia was detected in the digestate, indicating biological instability, and this was successfully remedied by adding trace elements. The energy balance and coefficient of performance (COP) of the system were calculated, which concluded that the system used 49% less heat energy by being housed in a greenhouse, achieved a net positive energy balance and potential COP of 3.16 and 5.55 based on electrical and heat energy, respectively. Greenhouse gas emissions analysis concluded that the most important contribution of the plant to the mitigation of greenhouse gases was the avoidance of on-site fossil fuel use, followed by the diversion of food waste from landfill and that the plant could result in carbon reduction of 2.95 kg CO2eq kWh -1 electricity production or 0.741 kg CO2eq kg -1 waste treated. Highlights A micro-scale AD plant was built and operated reliably in London, UK  The system produced 0.596 m 3 CH4 kg -1 VS from locally-collected mixed organic waste  GHG reduction of the system was 0.741 kg CO2eq kg -1 waste treated cf. landfilling  The system advantageously included a pre-digestion tank to buffer the feed variations  Biological ammonia inhibition was mitigated by trace element supplementation

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“…13/14 C, 3 H, 15 N, 34 S, 33 P, 18 O), which makes secondary ion mass spectrometry (SIMS) become a constitutive alternative for MAR-FISH. However, in anaerobic digesters it has been used to elucidate metabolic functions of minor phylogenetic groups like Chloroflexi, Syntrophomonas Spirochaeta and Synergistes as well as Methanosaeta spp.…”

Section: Fluorescence In Situ Hybridization-based Confocal Laser Scanmentioning

confidence: 99%

“…Isotope ratio mass spectrometry (IRMS) based methods analyzing isotope discrimination by the biogas producing microbial community were recently proposed to detect methanogenic pathway shifts [17,18]. The switch from acetoclastic to hydrogenotrophic methanogenesis is interpreted as signal of stress conditions which may allow plant operators to adjust their feeding strategy.…”

Section: Gas Productionmentioning

confidence: 99%

Microbiology and Molecular Biology Tools for Biogas Process Analysis, Diagnosis and Control

Lebuhn

Weiß

Munk

et al. 2015

Biogas Science and Technology

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Many biotechnological processes such as biogas production or defined biotransformations are carried out by microorganisms or tightly cooperating microbial communities. Process breakdown is the maximum credible accident for the operator. Any time savings that can be provided by suitable early-warning systems and allow for specific countermeasures are of great value. Process disturbance, frequently due to nutritional shortcomings, malfunction or operational deficits, is evidenced conventionally by process chemistry parameters. However, knowledge on systems microbiology and its function has essentially increased in the last two decades, and molecular biology tools, most of which are directed against nucleic acids, have been developed to analyze and diagnose the process. Some of these systems have been shown to indicate changes of the process status considerably earlier than the conventionally applied process chemistry parameters. This is reasonable because the triggering catalyst is determined, activity changes of the microbes that perform the reaction. These molecular biology tools have thus the potential to add to and improve the established process diagnosis system. This chapter is dealing with the actual state of the art of biogas process analysis in practice, and introduces molecular biology tools that have been shown to be of particular value in complementing the current systems of process monitoring and diagnosis, with emphasis on nucleic acid targeted molecular biology systems.

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Stable isotope composition of biogas allows early warning of complete process failure as a result of ammonia inhibition in anaerobic digesters

Cited by 70 publications

References 35 publications

Impact of the substrate loading regime and phosphoric acid supplementation on performance of biogas reactors and microbial community dynamics during anaerobic digestion of chicken wastes

Impact of the substrate loading regime and phosphoric acid supplementation on performance of biogas reactors and microbial community dynamics during anaerobic digestion of chicken wastes

Assessment of micro-scale anaerobic digestion for management of urban organic waste: A case study in London, UK

Microbiology and Molecular Biology Tools for Biogas Process Analysis, Diagnosis and Control

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