Stable integration and expression of β-glucuronidase and NPT II genes in mango somatic embryos

Mathews, Helena; Litz, R. E.; Wilde, H. Dayton; Merkle, S. A.; Wetzstein, Hazel Y.

doi:10.1007/bf02823312

Cited by 47 publications

(21 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various modifications to the leaf disc transformation protocol have been made for the transformation of somatic embryos and PEMs. Mango (Magnifera indica) PEMs were macerated before inoculation (Mathews et al 1992). Peach (Prunus persica) and European chestnut (Castanea sativa Mill.)…”

Section: Introductionmentioning

confidence: 99%

Plate flooding as an alternative Agrobacterium-mediated transformation method for American chestnut somatic embryos

Rothrock

Polin-McGuigan

Newhouse

et al. 2006

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

In an attempt to improve Agrobacterium-mediated transformation frequency of American chestnut somatic embryos, a novel method of inoculation/co-cultivation was developed. Plate flooding is a simple method where the Agrobacterium inoculum is poured onto the embryos while they remain on multiplication medium. This method tested the hypothesis that wounding tissues prior to co-cultivation was unnecessary or counterproductive. Two clones, WB296 and P1-1, were tested for differences in transformation efficiency as measured by the number of transformed embryogenic cell lines per Petri dish, the total number of transformed cell lines (embryos plus callus) and percentage of transformants that remained embryogenic. Plate flooding using clone WB296 produced significantly more transformed embryo cell lines and had a higher percentage of transformants remain embryogenic. The number of total transformed cell lines (embryos plus callus) was the same as obtained by other methods (desiccation, blot dry, sand abrasion, sonication and vacuum infiltration). With clone P1-1 there were no significant differences among the inoculation/cocultivation treatments tested. Polymerase chain reaction and Southern hybridizations confirmed that the transgene of interest had been stably integrated into both American chestnut clones. Whole plants were regenerated from clone P1-1.

show abstract

Section: Introductionmentioning

confidence: 99%

Plate flooding as an alternative Agrobacterium-mediated transformation method for American chestnut somatic embryos

Rothrock

Polin-McGuigan

Newhouse

et al. 2006

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…In citrus (Dutt and Grosser 2010), sandalwood (Shekhawat et al 2008), and yew (Zhang et al 2011), cell suspension cultures have been used to generate more responsive target explants, although putatively transformed explants were selected on semi-solid medium. Cell suspension cultures have been used during the selection phase in mango (Mathews et al 1992), avocado (Cruz-Hernandez et al 1998;Raharjo et al 2008) and olive (Torreblanca et al 2010), although no clear comparison was made with selection on semi-solid medium. An efficient transformation protocol involving the use of embryogenic suspension cultures throughout the whole procedure has been established for American chestnut (Fagaceae) (Andrade et al 2009).…”

Section: Discussionmentioning

confidence: 99%

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Mallón

Viéitez

Vidal

2013

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

As the limiting factor for recovery of plants from oak embryogenic lines is the low embryo conversion rate, axillary shoot lines were established from transformed germinated embryos.Transformed embryos and shoots were cultured in medium with or without kanamycin and the responses to several morphogenetic processes (recovery after cryopreservation, germination, shoot proliferation, and rooting) were evaluated.2

show abstract

“…This system has potential use in gene transformation and for scale-up and synchronization of embryogenesis. Such fine repetitively embryogenic tissue is amenable to transformation, and can be used in selection protocols, where it can eliminate transegenic chimeras (Mathews et al 1992). Repetitive embryogenesis also allows for continued high-frequency embryogenesis and long-term regeneration of transformed lines.…”

Section: Genetic Transformationmentioning

confidence: 99%

Carya illinoensis (Pecan)

Wetzstein

Rodriguez²,

Burns³

et al. 1996

Biotechnology in Agriculture and Forestry

View full text Add to dashboard Cite

Stable integration and expression of β-glucuronidase and NPT II genes in mango somatic embryos

Cited by 47 publications

References 21 publications

Plate flooding as an alternative Agrobacterium-mediated transformation method for American chestnut somatic embryos

Plate flooding as an alternative Agrobacterium-mediated transformation method for American chestnut somatic embryos

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Carya illinoensis (Pecan)

Contact Info

Product

Resources

About