Carya illinoensis (Pecan)

Wetzstein, Hazel Y.; Rodriguez, Adriana Pinheiro Martinelli; Burns, J. A.; Magner, Hazel N.

doi:10.1007/978-3-662-10617-4_4

Cited by 8 publications

(8 citation statements)

References 25 publications

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“…PLANT MATERIAL. Embryogenic pecan cultures were initiated and maintained as previously described (Mathews and Wetzstein, 1993;Wetzstein et al, 1996). Briefly, cultures were initiated from Pecan (Carya illinoinensis) is an economically important nut crop that could benefit from development of an efficient, mass clonal propagation system.…”

Section: Methodsmentioning

confidence: 99%

Field Performance and Molecular Evaluations of Pecan Trees Regenerated from Somatic Embryogenic Cultures

Vendrame¹,

Kochert²,

Sparks³

et al. 2000

jashs

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Field evaluations were conducted of pecan [Carya illinoinensis (Wangenh.) C. Koch] trees regenerated via somatic embryogenesis to assess if the trees maintained clonal fidelity and exhibited true-to-type characteristics. Phenotypic and molecular comparisons were made of trees from two different tissue culture lines after 4 years in the field. Factors evaluated included shoot growth, leaf morphology, and susceptibility to fungal scab [Cladosporium caryigenum (Ellis & Langl.) Gottwald] and southern pecan leaf phylloxera (Phylloxera russellae Stoetzel). Genetic fidelity was examined using amplified fragment length polymorphism (AFLP) analysis. Statistically significant differences were observed between the culture lines in phenotypic leaf characteristics (i.e., specific leaf weight and leaf length-to-width ratio), number of shoots per 1-year-old branch, and in the frequency of scab lesions on leaves. No between-line differences were observed in trunk caliper, average and total shoot growth, shoot length per cross-sectional area, or presence of phylloxera galls. AFLP analysis readily detected differences between culture lines. Cluster analysis generally grouped trees together that were regenerated from the same line. Trees within a culture line usually exhibited similar leaf characteristics, but not shoot growth or tree height. A few trees exhibited more extreme leaf characteristics and differed from each other. However, they were statistically similar to most of the other trees in the population evaluated. AFLP data revealed that some trees exhibited greater divergence and less similarity than other trees from the same line. The nature and significance of such variation at this time are not related to any detectable phenotypic differences.

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Section: Methodsmentioning

confidence: 99%

Field Performance and Molecular Evaluations of Pecan Trees Regenerated from Somatic Embryogenic Cultures

Vendrame¹,

Kochert²,

Sparks³

et al. 2000

jashs

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“…Slow drying for 4-6 d, resulting in a 12-15% loss of fresh weight, has been reported to stimulate the germination of Ocotea catharinensis somatic embryos more than 2 h rapid desiccation in a flow chamber (which reduced fresh weight by 57%) (Viana and Mantell 1999). Slow drying for 5 d has also been used for germination of pecan somatic embryos (Wetzstein et al 1996), and Black (1999) found that reducing the initial water content of wheat embryos from 73% to 69% of fresh weight was sufficient to induce desiccation tolerance, which they tentatively attributed to the triggering of tolerance mechanisms by the reduction in turgor and alteration of osmotic balance. That a degree of desiccation can trigger rapid biochemical changes is supported by the increase in plant regeneration achieved by partial desiccation of rice embryogenic callus, which was related to differences in soluble protein patterns as early as 1 d after desiccation treatment (Rance et al 1994); water-stress-induced proteins may be playing a key role in water stress tolerance and improvement of somatic embryo maturation (Malabadi and Nataraja 2006).…”

Section: )mentioning

confidence: 99%

Improved germination of somatic embryos and plant recovery of European chestnut

Corredoira

Valladares

Viéitez

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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For the mass production of chestnut trees with selected, hybrid, or genetically engineered genotypes, one potentially desirable propagation strategy is based on somatic embryogenesis. Although methods exist for the initiation of embryogenic cultures of Castanea sativa from immature zygotic embryos or leaf explants, the embryos produced have had low rates of conversion into plantlets. This study explored the possible benefits for somatic embryos that have already undergone maturation and cold treatments, of (a) partial slow or fast desiccation, and (b) of the addition of plant growth regulators or glutamine to the germination medium. Germination response was evaluated in terms of both conversions to plantlets and through embryos developing only shoots (shoot germination) that could be rooted following the micropropagation protocols developed for chestnut. Two or 3 wk slow desiccation in sealed empty Petri dishes resulted in a slight reduction in water content that nevertheless increased total potential plant recovery, shoot length, and the number of leaves per plantlet. However, best results were achieved by 2 h fast drying in a laminar flow hood, which reduced embryo moisture content to 57-58% and enhanced the potential plant recovery and quality of regenerated plantlets. Plant yield was also promoted by addition of 0.44 μM benzyladenine and 200-438 mg/l of glutamine to the germination medium, and plantlet quality (as evidenced by root, shoot, and leaf growth) by the further addition of 0.49 μM indole-3-butyric acid.

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“…The sterilization of the buds excised from mature plants gave unsatisfactory results or has required laborious treatments. In contrast, low levels of contamination have been obtained using immature seeds as explants (Wetzstein et al, 1996). Table 1 shows the detailed protocols adopted for several explant tissues of Pecans by several workers.…”

Section: Sterilization Of Explantsmentioning

confidence: 99%

“…It has several applications such as mass clonal propagation, genetic transformation and use in studies of embryo development. Due to somatic embryogenesis, it is possible to propagate clonal Pecan rootstocks introducing genes of commercial interest such as dwarfing for size control, enhanced nutrient uptake, alternate bearing control, salinity tolerance, nematode resistance and growth uniformity (Wetzstein et al, 1996). The first studies of somatic embryogenesis date back to 1987, when Merkle et al (1987) obtained somatic embryos using immature nuts as explants.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Propagation of Pecan (Carya illinoensis): A review

Casales¹,

Watt²,

Coetzer³

2018

Afr. J. Biotechnol.

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The Pecan (Carya illinoensis) is the most important species that belongs to the Carya genus. It is cultivated mainly for its nut, which is rich in oils and proteins, and for its good quality wood. Pecan is conventionally propagated by budding or grafting onto rootstocks obtained by open pollination. However, these techniques are not very efficient due to low propagation rates, poor survival and difficult establishment. Therefore, in vitro propagation of pecan can play a very important role in rapid multiplication of cultivars with desirable traits and production of healthy and disease-free plants in a short time. In the present review, the improvements over the years in the propagation of pecans, the refinements of protocols for obtaining high shoot multiplication and regeneration through organogenesis and/or somatic embryogenesis is discussed. Some basics of genetic transformation and its possible benefits are also discussed.

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Carya illinoensis (Pecan)

Cited by 8 publications

References 25 publications

Field Performance and Molecular Evaluations of Pecan Trees Regenerated from Somatic Embryogenic Cultures

Field Performance and Molecular Evaluations of Pecan Trees Regenerated from Somatic Embryogenic Cultures

Improved germination of somatic embryos and plant recovery of European chestnut

Propagation of Pecan (Carya illinoensis): A review

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