Stable genetic transformation of Jatropha curcas via Agrobacterium tumefaciens-mediated gene transfer using leaf explants

Kumar, Nitish; Anand, Kanika; Pamidimarri, D. V. N. Sudheer; Sarkar, Tanmoy; Reddy, M. Venkat Ram; Radhakrishnan, T.; Kaul, Tanushri; Reddy, M. K.; Sopori, Sudhir K.

doi:10.1016/j.indcrop.2010.03.002

Cited by 65 publications

(41 citation statements)

References 29 publications

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“…The percentage of rooting was directly proportional to the concentration of IBA and a higher percentage was observed in combinations of auxins as compared to IBA alone. Similar observations have been observed in Simmondsia chinensis ) and J. curcas (Kumar and Reddy 2010;Kumar et al 2010c). The acclimatization of the rooted shoots was easily accomplished and more than 90% of the plants were successfully transferred to polythene bags in under greenhouse conditions.…”

Section: Discussionsupporting

confidence: 81%

Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants

Kumar

Anand

Reddy

2011

J. Plant Biochem. Biotechnol.

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Section: Discussionsupporting

confidence: 81%

Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants

Kumar

Anand

Reddy

2011

J. Plant Biochem. Biotechnol.

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“…Low genetic variability within domestic jatropha germplasm collections was also reported worldwide in studies using SSRs (Pamidimarri et al 2009, Kumar et al 2010 and ISSRs (Achten et al 2010). Mukherjee et al (2011) observed approximately 75% of genetic similarity among jatropha germplasm collections from all over the world.…”

Section: Genetic Diversity Of Jatropha Acessions In Northern Minas Gementioning

confidence: 55%

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Santos

Ferreira

Setotaw

et al. 2016

An. Acad. Bras. Ciênc.

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Jatropha is a potential oilseed crop, which requires mitigating factors such as the low genetic variability of the species. The solution runs through the research of Brazilian germplasm. Attention should be given to the germplasm of jatropha the north of Minas Gerais, because this is the oldest national collection and because this region may be a regions of jatropha diversity due to selection pressure arising from environmental adversities. Therefore, the objective of this study was to investigate the genetic diversity of 48 accessions of collection from Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), using SSR and ISSR markers. The results showed low genetic diversity, but some individuals stood out as J. mollissima (48), J. podagrica (47), Mexican accessions (42, 43, 44 and 45) and some national accessions (28, 29, 41 and 46). Therefore, aiming to increase the genetic variability and improve the effectiveness of jatropha breeding programs, it is suggested to explore such as parental accessions to generate commercial hybrids. This fact implies the possibility to support future production of jatropha, since this culture may be an important source of income, especially for small farmers living in semiarid regions of Brazil.

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“…For rooting of the elongated shoots, shoots at least 1 cm in length were isolated from the mother tissues and inoculated onto fresh MS medium supplemented with 0.3 mg/L indole-3-butyric acid (IBA; SigmaÀAldrich Co., St Louis, MO, USA) and different concentrations (0, 4,8,16,32,64 and 128 mg/L) of L-glutamine (Gln; SigmaÀAldrich Co., St Louis, MO, USA) and the results were assessed on 10, 20, 30 and 40 days of culture. The Gln solution was filtersterilized and added to the autoclaved culture medium as described previously.…”

Section: Rooting Culturementioning

confidence: 99%

Efficient culture protocol for plant regeneration from petiole explants of physiologically mature trees ofJatropha curcasL.

Liu

Tong

Hui

et al. 2015

Biotechnology & Biotechnological Equipment

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An efficient and reproducible protocol for induction of adventitious shoot buds and plant regeneration from petiole explant cultures of Jatropha curcas, an important biofuel crop, is described. Physiologically mature trees of three J. curcas genotypes were selected and explants were prepared from young petioles. Treating the explants with high concentrations (5 to 120 mg/L) of thidiazuron (TDZ) solution for short time periods (5 to 80 min) helped increase the regeneration frequency and improved the quality of the regenerated buds significantly. The age of the petioles and inoculation methods were found to influence the culture results. The best shoot buds induction (65.78%) and number of buds (6.77) per explant was seen in the second petiole explants of genotype M-1 treated with 20 mg/L TDZ solution for 20 min, followed by 35-day culture on hormone-free Murashige and Skoog medium. The regenerated buds could elongate to become shoots in a medium containing gibberellic acid. The elongated shoots initiated roots to become intact plantlets in rooting medium containing indole-3-butyric acid and L-glutamine (Gln), and supplementing 16 mg/L Gln into the rooting medium effectively stimulated the initiation and growth of roots, with the best rooting rate (51.72%). After acclimatization, these plantlets were transplanted to soil wherein normal growth was observed. Therefore, an intact plantlet could usually be obtained at 60 days of culture by using the culture protocol described in this study. This protocol can be used for mass production of true-to-type plants and the production of transgenic plants through Agrobacterium/biolistic-mediated transformation.

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Stable genetic transformation of Jatropha curcas via Agrobacterium tumefaciens-mediated gene transfer using leaf explants

Cited by 65 publications

References 29 publications

Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants

Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement

Efficient culture protocol for plant regeneration from petiole explants of physiologically mature trees ofJatropha curcasL.

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