Efficient culture protocol for plant regeneration from petiole explants of physiologically mature trees of<i>Jatropha curcas</i>L.

Liu, Ying; Tong, Xin; Hui, Wenkai; Liu, Tong; Chen, Xiaoyang; Li, Jing; Zhuang, Chuxiong; Yang, Yankun; Liu, Zhenlan

doi:10.1080/13102818.2015.1013308

Cited by 30 publications

(32 citation statements)

References 50 publications

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“…For example, the use of petioles of broccoli as explant material gives a high degree of monomorphism [20]. In J. curcas, a few studies have used petioles as explant material for micropropagation [9,10,16]. However, to the best of our knowledge, there are no reports on the genetic stability in petiole explants of J. curcas.…”

Section: Resultsmentioning

confidence: 89%

“…According to Liu et al [10], petioles that are too young or too old are not suitable as starting material, since they have low regeneration frequency. Furthermore, petioles that are too young are susceptible to sterilization agents and lead to the death of the tissue.…”

Section: Resultsmentioning

confidence: 99%

“…Micropropagation is the best technique to produce a large amount planting material with desired traits in a short time and small space. The micropropagation of J. curcas has been reported from various explants, such as nodes [7], shoot tips [8], petioles [9,10], leaves [11], hypocotyls [12] and embryos [13]. The establishment of Jatropha plants from various explants will enhance the production of plant material from the elite mother plant.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Nasir

Anuar

Zainuddin

et al. 2016

Biotechnology & Biotechnological Equipment

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Genetic homogeneity is known to be the most important prerequisite in the micropropagation of Jatropha curcas L. to produce true-to-type plants. The detection of genetic homogeneity in clonal micropropagation for elite plants at an early stage is required, to avoid any increase in variation in the next stage of micropropagation. The genetic homogeneity was assessed during shoot bud formation from petiole explants of J.curcas (P1 £ P3) hybrid with different concentrations of thidiazuron (TDZ) in a range of 0.5-4.0 mg/L using inter simple sequence repeat (ISSR) markers. Out of 23 ISSR primers, 16 primers produced clear, distinct and reproducible bands. A total of 96 bands, ranging in size from 100 to 1013 bp were generated. Based on the band data, a total of 94 bands were monomorphic (98%) and two bands were polymorphic (2%). All banding patterns from the shoot buds induced by 0.5, 1.0 and 2.0 mg/L TDZ were monomorphic, but 4.0 mg/L gave 2% polymorphism. These findings indicated that concentrations of 0.5, 1.0 and 2.0 mg/L TDZ did not trigger any somaclonal variation and could, therefore, be considered suitable for application in clonal micropropagation of J. curcas hybrid using petioles as explant material.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Nasir

Anuar

Zainuddin

et al. 2016

Biotechnology & Biotechnological Equipment

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“…Mature seeds were collected sufficiently from the M-19 J. curcas trees, which were planted well in a farm of Haikou, Hainan province, China [5].…”

Section: Plant Sourcesmentioning

confidence: 99%

“…Nevertheless, most cases for plant regeneration of J. curcas were quite time-consuming (90-140 days) for obtaining regenerated plantlets [5]. For breeding excellent varieties of J. curcas and large-scale production of high quality planting material, high-efficiency tissue culture protocols are needed greatly.…”

Section: Introductionmentioning

confidence: 99%

Increase of Regeneration Efficiency in JATROPHA CURCAS by In Vitro Culture Methods

Liu¹,

Lu²,

Niu³

et al. 2016

Proceedings of the 3rd International Conference on Material Engineering and Application (ICMEA 2016)

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Abstract. An effective protocol has been established to induce adventitious buds regeneration from 3 types of explants (petiole, petiolule, cotyledon petiole) of Jatropha curcas L. All the explants were dealt with high concentrations (5-120 mg/L) of TDZ solution for various soak time (5-80 min) with the regeneration frequency increased and the quality of the regenerated shoot-buds improved significantly compared with the traditional regeneration approach. The best result of adventitious buds regeneration was observed when explants were treated with 20 mg/L TDZ solution for 20 min before being placed on hormone-free MS medium for 30 days of culture. Moreover, addition of GA3 to the elongation medium was contributed to the prolongation of the shoot buds and the best elongate results could be gained at a concentration of 0.4 mg/L. By application of the new protocol described in this paper, plant regeneration efficiency of J. curcas was increased observably and the culture period was shortened in 65 days.

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Effect of different factors on plant regeneration from leaf explants of Jatropha curcas L.

Liu

Chen

et al. 2020

Agronomy Journal

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The in vitro regeneration frequency was commonly low, and the quality of adventitious buds was poor, when the traditional methods were used to induce adventitious buds from leaf explants of Jatropha curcas L. through the medium containing low concentration of thidiazuron (TDZ). When treated with high concentrations (6.5-104 mg/L) of TDZ for a certain time (7-120 min), the regeneration frequency and quality of adventitious buds were significantly increased. The best induction rate of adventitious buds (78.58%) and the average number of buds per explant (7.26) were obtained by soaking leaf explants in 26 mg/L TDZ solution for 30 min and then transferring them onto MS medium without hormone for 30 days. Furthermore, the adventitious bud regeneration was affected obviously by source of explants, sterilization time, illumination time and genotype. The subsequent elongation and rooting experiments showed that adventitious bud elongation could be improved by adding diethyl aminoethyl hexanoate (DA-6) in the medium, and the best elongation effect could be gained when the concentration of DA-6 was 1.2 mg/L. The elongated shoots induced the roots and developed into small intact plants on the MS medium with 1.5 mg/L sodium nitroprusside (SNP). After domestication, these small plants could be transplanted into the soil and growing normally. Therefore, the method established in this study was helpful to improve the plant regeneration efficiency of leaf explants in J. curcas.

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Efficient culture protocol for plant regeneration from petiole explants of physiologically mature trees ofJatropha curcasL.

Cited by 30 publications

References 50 publications

Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Increase of Regeneration Efficiency in JATROPHA CURCAS by In Vitro Culture Methods

Effect of different factors on plant regeneration from leaf explants of Jatropha curcas L.

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