Stable expression of hepatitis B surface antigen gene inDunaliella salina(Chlorophyta)

Transient Gene Expression System Established in Porphyra yezoensis Is Widely Applicable in Bangiophycean Algae

“…Stable transformation systems have already been established in microalgae (Hallmann 2007), such as volvocine green algae, unicellular Chlamydomonas reinhardtii (Debuchy et al 1989;Kindle et al 1989), Dunaliella salina (Geng et al 2003(Geng et al , 2004) and multicellular Volvox carteri (Schiedlmeier et al 1994), all of which have long been favorite experimental organisms for genetic and molecular biological studies. Recently, eukaryotic green alga Ostreococcus tauri, which is an attractive microalgal model because of its minimal cellular organization and compact genome, has also become transformable (Corellou et al 2009;Moulager et al, 2010;Heidje et al, 2010).…”

Utility of the pat gene as a selectable marker gene in production of transgenic Dunaliella salina

“…However, the concentration observed in our study was much lower than that observed in several other studies. Geng et al (2003) reported that D. salina died in 3 weeks in 60 μg/ml chloramphenicol, and Tan et al (2005) reported that D. salina died in 400 μg/ml chloramphenicol. These discrepancies are attributed to differences in the cell densities used and the periods of antibiotic treatment.…”

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“…Successful expression and assembly of a recombinant human monoclonal IgA antibody has already been demonstrated for Chlamydomonas reinhardtii [6], while stable expression of the hepatitis B surface antigen gene has been shown in Dunaliella salina [7]. Since Dunaliella is otherwise used for nutrition, there is no need for purification of the antigen, so the intact algae could be used to deliver a vaccine.…”