Stabilization of Red Blood Cells by the Plasticizer, Diethylhexylphthalate

Self Cite

The biochemical status of whole blood stored in containers fabricated of ethylene ethylacrylate (EEA) film was monitored at several times during 4 weeks of storage at 4°C. Fifteen biochemical indicators were studied to reflect on erythrocyte integrity, cellular metabolism, plasma protein stability, and microaggregate formation. Comparison to storage in polyvinyl chloride (PVC) containers was made by distributing aliquots from each unit of blood among the containers being compared. Whole blood in EEA developed significantly higher levels of plasma hemoglobin, erythrocyte osmotic fragility, and D-glycerate- 2,3-diphosphate (2,3-DPG), and somewhat greater glucose utilization, lactate production, and pH. These biochemical differences were not of great magnitude and the data suggest that EEA containers are compatible with the storage of whole blood.

Section: Discussionmentioning

confidence: 99%

Biochemistry of Whole Blood in Poly(Ethylene-Co-Ethylacrylate) Experimental Blood Containers

Horowitz¹,

Rowe²,

Stryker³

et al. 1985

Self Cite

“…Similar results have been reported by Stern and Carmen [13], who showed that DEHP added to blood either by extraction from the container material or as a solution in plasma produced significant protection against hemolysis during storage. Horowitz et al [14] have found comparable results when comparing containers made of PVC film and DEHP platicizer with another plastic poly, ethylene-co-ethylacrylate (EEA). They feel that DEHP has a stabilizing effect on red cell membranes.…”

Section: Discussionmentioning

confidence: 99%

Survival of Red Cells Stored for 21 and 35 Days in a Non-Di-(2-Ethylhexyl)Phthalate Plastic Container

Myhre¹,

Johnson²,

Marcus³

et al. 1987

Whole blood and red cells were stored using citrate-phosphate-dextrose (CPD) and citrate-phosphatedextrose- adenine (CPDA-1) anticoagulants in polyvinylchloride bags made flexible with di-(2-ethylhexyl)phthalate (DEHP) or tri-(2-ethylhexyl)trimellitate (TOTM) plasticizers. After storage the posttransfusion viability of these cells was tested in autologous donors. Cells stored in TOTM-plasticized film had a survival rate less than 75% when stored for 35 days, while other systems had a survival greater than this. When compared with the red cells stored in CPD-DEHP-plasticized film, the viability of whole blood and red cells stored in CPDA-TOTM showed a statistically significant decrease (p = <0.01). Therefore, red cell storage in TOTM-plasticized PVC with current anticoagulant should be limited to 21 days.

“…reducing haemolysis [1][2][3] and increasing in vivo survival [4], However, like other lipid-soluble PVC plasticisers, DEHP comprises 3(M10% of final polymer weight, is not covalently bound within the PVC matrix, and leaches from the pack into blood and plasma [5], DEHP has been shown to have toxic effects in animal studies, whether transfused intravenously or administered orally and may be linked to Both red cells and platelet concentrates (PCs) from whole blood donations were formerly prepared and stored in polyvinyl chloride (PVC) first-generation containers plasticised with diethylhexyl phthalate (DEHP). This plas ticiser was shown to confer stability on red cell membranes, study compared PRP-derived PCs stored in PVC-BTHC (PL 2209) with those stored in PVC-TEHTM (PL 1240) as control, the latter being in current use at this centre.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Platelets Stored in Polyvinyl Chloride Containers Plasticised with Butyryl Trihexyl Citrate or Triethylhexyl Trimellitate

Turner¹,

Mitchell²,

Kang³

et al. 1995

Platelet concentrates (PCs), stored for 5 days in PL 2209, a new polyvinyl chloride (PVC) storage container plasticised with butyryl trihexyl citrate, were compared with those stored in PL 1240, a PVC platelet container plasticised with triethylhexyl trimellitate. In part 1 of the study, pooled platelet-rich plasma (PRP) was aliquoted into each type of pack and pH, pCO(2), pO(2), hypotonic shock response, aggregation responses, lactate, glucose and ATP concentrations, and lactate dehydrogenase and β-thromboglobulin release were compared at days 1, 3 and 5. In part 2,12 volunteers gave a unit of blood on two separate occasions and PCs produced by the PRP method were stored in PL 2209 or PL 1240 for 5 days before autologous reinfusion of a 111In-labelled sample. In vitro results demonstrated that PL 2209 was more gas permeable than PL 1240. In part 2 of the study, at day 5, pCO(2) was 3.13±0.62 versus 5.14±0.69 (p<0.001), whilst pO(2) was not significantly different for PL 2209 versus PL 1240, respectively. pH was better maintained in PL 2209 than in PL 1240 (7.38±0.13 vs. 7.24±0.10, respectively, p < 0.01) after storage for 5 days. These results were confirmed by those from part 1. In vivo data were similar for PC stored in the two plastics with a multiple- hit recovey of 40.9±12.1% for PL 2209 and 37.4+11.3% for PL 1240, and a multiple-hit survival of4.89±1.20 days and 5.28±2.06 days for PL 2209 and PL 1240, respectively. γ-Camera imaging of volunteers showed similar biodistribution of radiolabelled platelets stored in each container. These results demonstrate that PL 2209 is a suitable container for storage of PCs for 5 days.