Stabilization of hydrochlorothiazide nanocrystals using fibroin

Abstract

Nanocrystals of the poorly water soluble drugs is a promising strategy. To stabilize the drug nanocrystals, stabilizers are usually used; however, the use of common stabilizers is limited by weak stabilization effect and toxicological concerns for long-term treatment. The present work was aimed to investigate the potential of natural silk protein as novel stabilizer for nanocrystal of hydrochlorothiazide, which was a model drug. The nanocrystals of drug with hydrophobic protein stabilizer were prepared by antisolvent precipitation method. Prepared nanocrystals were evaluated for parameters like particle size, zeta potential, DSC, XRD, %crystallinity, SEM, drug content and in vitro dissolution test. Stabilization efficiency of nanocrystals was assessed by their % crystallinity for 3 months. Optimized batch R2 was shown smaller particle size, highest drug content and drug dissolution. Nanocrystals were shown the extended release due to coat of fibroin around the hydrochlorothiazide. From the % crystallinity study it was found that there was not significant change in the nanocrystals prepared using fibroin. So, it was concluded that the fibroin is good stabilizer for drug nanocrystals.

Nanoteknologi Farmasi Ziprasidone Nanocrystals by Wet Media Milling Followed by Spray Drying Andlyophilization: Formulation and Process Parameter Optimization

“…Dapat diproduksi baik dengan teknik presipitasi (bottom-up approach) atau dengan pengurangan ukuran (top-down approach). Nanokristal obat adalah partikel nano yang terdiri dari 100% obat tanpa bahan matriks dan rata-rata ukuran partikel di bawah 1µm (Dhole et al, 2019).…”