Stabilization of glucoso-6-phosphate dehydrogenase by its substrate and cofactor in an ultrasonic field

Karaseva, E. I.; Metelitza, D. I.

doi:10.1134/s1068162006050062

Cited by 15 publications

(6 citation statements)

References 2 publications

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“…Осмотичну резистентність визначали за концентрацією гіпотонічного розчину натрію хлориду. В еритроцитах досліджували: концентрацію відновленого глутатіону (Hernayanti et al, 2015), активність глюкозо-6-фосфатдегідрогенази (КФ 1.1.1.49) (Karaseva and Metelitza, 2006) і глутатіонредуктази (КФ 1.6.4.2) (Chen and Nam, 2016).…”

Section: матеріал і методи дослідженьunclassified

“…Вказану реакцію каталізує глюкозо-6-фосфат. Саме ця реакція в основному забезпечує еритроцит необхідною кількістю відновленого нікотинамідаденін-динуклеотидфосфату, найважливішого донатора водню, що використовується для відновлення глутатіону (Karaseva and Metelitza, 2006). Відновлення глутатіону до глутатіондисульфіду відбувається за безпосередньої участі глутатіонредуктази, основна функція якої полягає у захисті сульфгідрильних груп білків, зберігаючи їх у відновленому стані.…”

Section: а бunclassified

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Mechanism of changing adaptation potential and morpho-biochemical parameters of erythrocytes in students with different modes of daily activity after physical loading

Pоpеl¹,

Mytckan²,

Lapkovskyi³

et al. 2017

Regul. Mech. Biosyst.

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The aim of this work was to identify morpho-functional and biochemical changes in erythrocytes in students with different daily educational -training regimens after dosed physical exertion. The study involved 50 male students aged 20–22 years who study at the Faculty of Physical Education and Sports. Of these, 25 were untrained students (control group) and 25 were students studying for the highest sports qualification who were engaged in sports in which training was conducted to improve overall endurance (skiing, biathlon, long-distance running). At the end of the school year, all students were required to carry out a single session involving physical loading of maximum intensity on a veloergometer. According to the results of the questionnaire, it was found that the combination of intensive training and training processes caused a mismatch between the functional capabilities of the students’ and athletes’ biological systems and the increased volume and intensity of their psycho-physical loading, which is closely correlated with a decrease in their level of physical health. In the blood of untrained students, under the influence of dosed physical activity, reversibly altered forms of erythrocytes appeared, which accounted for 2.0% of the total number of erythrocytes. In the morphological study of student athletes, an increase in the content of reversibly and irreversibly altered erythrocytes was observed, the number of which exceeded 2.0% of the total number of erythrocytes. In individual student athletes echinocytes with small-focal microdefects of the outer membrane were observed in the peripheral blood, as well as acanthocytes and stomatocytes, which have low resistance to acid hemolysis. The mechanism of this phenomenon is a decrease in the activity of glucose-6-phosphate dehydrogenase and glutathione reductase, which leads to a deficiency of reduced glutathione in erythrocytes. Such cells under the influence of oxidizing agents quickly undergo lysis, which is manifested by a violation of their structural integrity. This can lead to the development of “anemia of loading”, which is manifested by a decrease in the concentration of hemoglobin and the number of erythrocytes with their morphometric recalibration due to the appearance of macro-microcytic forms. The presence of such changes can adversely affect both the athletic performance and the state of health of students. This requires appropriate correction of the training process, as well as the development and timely application of adequate measures aimed at eliminating the development of the pathological process and eliminating possible undesirable consequences.

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Section: матеріал і методи дослідженьunclassified

Section: а бunclassified

Mechanism of changing adaptation potential and morpho-biochemical parameters of erythrocytes in students with different modes of daily activity after physical loading

Pоpеl¹,

Mytckan²,

Lapkovskyi³

et al. 2017

Regul. Mech. Biosyst.

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“…It also changes the saturation of blood with oxygen. According to Karaseva & Metelitza data [19], this is due to a interrelation change of the haemoglobin with 2,3-BPG.…”

Section: Discussionmentioning

confidence: 92%

Changes in the oxygen transport system of erythrocytes in testing the general endurance of students

Neporadna

Pоpеl

2019

PPS

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The aim of the study: to reveal morpho-functional changes in erythrocytes in students with different levels of general endurance. Material and methods: It was examined 80 students (17-19 years old). The students performed a 12-min shuttle run (20m endurance shuttle-run - bleep test). The ventilation function of the lungs was estimated according to the forced exhalation data. The gas composition of the blood was determined by the gas analyzer ОРТІ ССА-TS. Results. It was determined that in students with lower than average and low levels of general endurance, the increase of lungs ventilation is stipulated by the increase in the frequency of respiration. This leads to rapid tiredness of the respiratory muscles, reduces ventilation of the lungs and gas exchange. Low levels of blood oxygenation, intensity of oxidative phosphorylation and ATP resynthesis lead to the appearance of atypical forms of erythrocytes. It is established that at the low level of cardiorespiratory endurance there is an increase in the concentration of acidic metabolism products in arterial blood and reduce in its alkaline reserves. Conclusions. Students with below average and low levels of physical fitness are characterized by: increased respiratory rate, reduced ventilation of the lungs and gas exchange. This leads to the formation of a hypoxic condition in the peripheral blood: a reduce of pO2, pCO2 level and the appearance of metabolic acidosis symptoms. For students with below average and low level of physical fitness hypoxic condition leads to violation of bioenergetic processes in peripheral blood erythrocytes, reduction of ATP product, increase in the content of intra-erythrocytic 2,3-BPG.

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“…Stabilization of enzymes by substrates and analogs is a wellknown phenomenon that is commonly utilized in industrial enzymology (Haard, 1998;Iyer and Ananthanarayan, 2008;Karaseva and Metelitza, 2006;Robertus et al, 1972). However, the mechanism by which substrate stabilization occurs is not well understood, nor is it universal.…”

Section: Discussionmentioning

confidence: 99%

Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target

et al. 2018

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Protein dynamics manifested through structural flexibility play a central role in the function of biological molecules. Here we explore the substrate-mediated change in protein flexibility of an antibiotic target enzyme, Clostridium botulinum dihydrodipicolinate synthase. We demonstrate that the substrate, pyruvate, stabilizes the more active dimer-of-dimers or tetrameric form. Surprisingly, there is little difference between the crystal structures of apo and substrate-bound enzyme, suggesting protein dynamics may be important. Neutron and small-angle X-ray scattering experiments were used to probe substrate-induced dynamics on the sub-second timescale, but no significant changes were observed. We therefore developed a simple technique, coined protein dynamics-mass spectrometry (ProD-MS), which enables measurement of time-dependent alkylation of cysteine residues. ProD-MS together with X-ray crystallography and analytical ultracentrifugation analyses indicates that pyruvate locks the conformation of the dimer that promotes docking to the more active tetrameric form, offering insight into ligand-mediated stabilization of multimeric enzymes.

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Stabilization of glucoso-6-phosphate dehydrogenase by its substrate and cofactor in an ultrasonic field

Cited by 15 publications

References 2 publications

Mechanism of changing adaptation potential and morpho-biochemical parameters of erythrocytes in students with different modes of daily activity after physical loading

Mechanism of changing adaptation potential and morpho-biochemical parameters of erythrocytes in students with different modes of daily activity after physical loading

Changes in the oxygen transport system of erythrocytes in testing the general endurance of students

Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target

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