Stability studies of amphetamine and ephedrine derivatives in urine

Jiménez, C.; Torre, Rafael de la; Ventura, Montse; Segura, Jordi; Ventura, Rosa

doi:10.1016/j.jchromb.2006.05.036

Cited by 50 publications

(41 citation statements)

References 36 publications

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“…Comprehensive stability studies by Jimé nez et al [12] as well as published work by other authors [22,35,36] have indicated the viable stability of amphetamine and related derivatives under various freeze-thaw and storage conditions. Freeze/thaw stability for MCA was also positively confirmed in agreement with reports in literature [22].…”

Section: Validationmentioning

confidence: 97%

“…Analytical strategies including gas chromatography (GC) [12,13], high performance liquid chromatography (HPLC) [14,15], gas chromatography-mass spectrometry (GC-MS) [10,12,[16][17][18][19][20][21] and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [22,23] have been employed. The majority of samples received by our laboratory are direct confirmation requests by clinicians.…”

mentioning

confidence: 99%

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Rapid GC–MS confirmation of amphetamines in urine by extractive acylation

Marais

Laurens

2009

Forensic Science International

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Section: Validationmentioning

confidence: 97%

mentioning

confidence: 99%

Rapid GC–MS confirmation of amphetamines in urine by extractive acylation

Marais

Laurens

2009

Forensic Science International

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“…The stability of AM in urine under different storage conditions has been demonstrated previously [25,30,31] and was therefore not assessed with the presented method.…”

Section: Validationmentioning

confidence: 94%

Accelerated quantification of amphetamine enantiomers in human urine using chiral liquid chromatography and on-line column-switching coupled with tandem mass spectrometry

et al. 2016

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Amphetamine (AM) is a powerful psychostimulant existing in two enantiomeric forms. Stereoselective analysis of AM in biosamples can assist clinicians and forensic experts in differentiating between abuse of illicitly synthesized racemic AM and ingestion of pharmaceutical AM formulations containing either S-AM or different proportions of the S-and R-enantiomers. Therefore, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying AM enantiomers in urine was newly developed. The method comprised dilution with water, followed by injection of the diluted sample onto an achiral C18 trapping column for purification and subsequent backflush elution to a chiral Lux 3 µm AMP LC column by means of a switching valve. An isocratic mobile phase of 25% acetonitrile in 0.1 M aqueous ammonia was used for enantiomeric separation. Injection, cleanup and backflush of the next sample were performed before the previous sample had eluted from the analytical column, thus enabling simultaneous enantioseparation of up to three samples within the analytical column. This novel chromatographic concept allowed for increased sample throughput by accelerating both the sample preparation and the LC analysis. Analyte detection was accomplished by electrospray ionization in positive ion mode and selected reaction monitoring using a triple-stage quadrupole mass spectrometer. The method was successfully validated through assessment of its linearity, lower limit of quantification, accuracy and precision, selectivity, matrix effect, carryover, dilution integrity, and re-injection reproducibility. Linearity ranged from 0.05 -25 mg/L for both enantiomers. Proof of the method included analysis of urine samples obtained from drug abusers and patients receiving an S-AM prodrug.

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“…whole blood, plasma, serum and/or urine), aliquoted and stored at the same time and in the same way as ordinary samples. The concentrations are measured at selected time intervals and compared to detect any trend regarding degradation [29][30][31][32][33][34][35][36][37][38][39][40]. Among reported investigations, also studies on authentic material from volunteers dosed with the drug or from laboratory cases have been performed [33,[40][41][42][43][44].…”

Section: Design and Evaluation Of Stability Experimentsmentioning

confidence: 99%

“…Furthermore, stability of some drugs in urine have been investigated such as 11-nor-Δ 9 -carboxy-tetra-hydrocannabinol acid (THCCOOH), THCCOOglu, amphetamine, methamphet-amine, ephedrine, morphine, codeine, cocaine, benzoylecgonine and/or phencyclidine [35,41,42,53], LSD) [59], the LSD metabolite 2-oxo-3-hydroxy lysergic acid diethylamide (O-H-LSD) [36] and GHB [44].…”

Section: Stability Investigations Of Drugs In Biological Specimensmentioning

confidence: 99%

Zopiclone degradation in biological samples : Characteristics and consequences in forensic toxicology

Nilsson¹

2014

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Stability studies of amphetamine and ephedrine derivatives in urine

Cited by 50 publications

References 36 publications

Rapid GC–MS confirmation of amphetamines in urine by extractive acylation

Rapid GC–MS confirmation of amphetamines in urine by extractive acylation

Accelerated quantification of amphetamine enantiomers in human urine using chiral liquid chromatography and on-line column-switching coupled with tandem mass spectrometry

Zopiclone degradation in biological samples : Characteristics and consequences in forensic toxicology

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