Stability of ZIF-8 Nanoparticles in Most Common Cell Culture Media

Abstract

Zeolite imidazolate framework-8 (ZIF-8) is a promising platform for drug delivery, and information regarding the stability of ZIF-8 nanoparticles in cell culture media is essential for proper interpretation of in vitro experimental results. In this work, we report a quantitative investigation of the ZIF-8 nanoparticle’s stability in most common cell culture media. To this purpose, ZIF-8 nanoparticles containing sterically shielded nitroxide probes with high resistance to reduction were synthesized and studied using electron paramagnetic resonance (EPR). The degradation of ZIF-8 in cell media was monitored by tracking the cargo leakage. It was shown that nanoparticles degrade at least partially in all studied media, although the degree of cargo leakage varies widely. We found a strong correlation between the amount of escaped cargo and total concentration of amino acids in the environment. We also established the role of individual amino acids in ZIF-8 degradation. Finally, 2-methylimidazole preliminary dissolved in cell culture media partially inhibits the degradation of ZIF-8 nanoparticles. The guidelines for choosing the proper cell culture medium for the in vitro study of ZIF-8 nanoparticles have been formulated.

A quest for cytocompatible metal organic frameworks in non-viral gene therapy: Relevance of zeolitic imidazolate framework-8

“…MILs are reported to be non-toxic, biodegradable nanocarriers suitable for antitumoral and retroviral drug delivery, imaging, and minimal in vivo toxicity [ 10 , 11 ]. ZIFs have been reported for drug and biomolecule delivery with demonstrated biocompatibility, biodegradability, protection, and encapsulation properties [12] , [13] , [14] , [15] , [16] . The most current bio-application of MOFs is in the field of gene therapy.…”

Bio‐Templated Chiral Zeolitic Imidazolate Framework for Enantioselective Chemoresistive Sensing

“…[35,36] It was reported that ZIF-8 shows high chemical stability toward the nitroxide compound. [37] Taking this fact into account, we can deduce that structure of chiral ZIF after full removal of CNCs with nitroxide compound, NMMO • H 2 O, would be same with that of chiral ZIF in the composite film. As can be seen in Figures 2c, 2f to 2h, S9, and S10, the templated grown ZIF has a needle-like morphology with a broad distribution of diameters and lengths around 84 nm and 270 nm, respectively.…”

“…Next, pure templated‐grown ZIF can be separated after full removal of the CNCs with N ‐methyl morpholine N ‐oxide monohydrate (NMMO ⋅ H 2 O) from the ZIF‐CNC film (Figures S4 and S5) [35,36] . It was reported that ZIF‐8 shows high chemical stability toward the nitroxide compound [37] . Taking this fact into account, we can deduce that structure of chiral ZIF after full removal of CNCs with nitroxide compound, NMMO ⋅ H 2 O, would be same with that of chiral ZIF in the composite film.…”

Functionalized ZIF‐8 with Aminopropyltrimethoxysilane as Montelukast Sodium Carrier for Medical Application

“…ZIF‐8 belongs to a family of MOFs with a high specific surface area, controllable pore size, functional pores, and a flexible chemical structure [28] and can be used as a host for a wide range of drugs. It has good biocompatibility [29] . Primarily, ZIF‐8 and APTES are used in combination to limit non‐uniform distribution, weak biomolecule interaction, and ion leaching on surfaces.…”

“…It has good biocompatibility. [29] Primarily, ZIF-8 and APTES are used in combination to limit non-uniform distribution, weak biomolecule interaction, and ion leaching on surfaces. The surfaces modified by a monolayer of APTESassembled amine molecules increase the nucleation of ZIF-8 and decrease their agglomeration, which leads to enhanced compatibility.…”