Stability of Two Mass-Reared Genetic Sexing Strains of Ceratitis capitata (Diptera: Tephritidae) Based on Pupal Color Dimorphisms

Busch-Petersen, E.; Kafu, A.

doi:10.1093/ee/18.2.315

Cited by 17 publications

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“…Since Kerremans et al (1990) found the translocation breakpoints on the autosomes in T:Y(wp)30C and T:Y(wp)101 to be probably identical, the difference between our estimate of spontaneous recombination and that of Busch-Petersen (1989) may result from differences in experimental design. However, a difference in recombination rate would fit in with the observation that the T:Y(wp)30C sexing strain is more stable than the T:Y(wp)101 strain under mass rearing conditions (Busch-Petersen & Kafu, 1989). At the same time, note must be taken of the fact that in computer simulations Hooper et al (1987) and Busch-Petersen & Baumgartner (1991) showed that the rate of breakdown in …”

Section: Discussionmentioning

confidence: 84%

“…and T : Y( wp +)30C in large rearing cages, containing 240,000 flies each, were reported by Hooper et al (1987) and Busch-Petersen & Kafu (1989). In these cases, the possibility of outside contamination has also to be borne in mind.…”

Section: Data On the Rates Of Breakdown Of T :Y(wp)10lmentioning

confidence: 85%

“…Strains based on Y chromosome translocations of a 'sexing' gene may become unstable through contamination by introduced foreign genotypes, because of recombination between the translocation breakpoint and the 'sexing' gene (Hooper et al, 1987;Busch-Petersen & Kafu, 1989) or the disruption of the translocation and reversion to the normal karyotype, as observed in the sheep blow fly, Lucilia cuprina (Wied.) (Foster et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

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Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

et al. 1993

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The frequency of male recombination (r) between the translocation breakpoint and the wp + gene, and the selection coefficient (s) against wp/ wp, in the genetic sexing strain T : Y( wp + )30C of the Mediterranean fruit fly Ceratitis capitata (Wied.) were measured in population experiments over 11 generations. The population was 50 females and 50 males in the first generation, after which 150 pupae were used to start each subsequent generation. A computer program was used to estimate values of r and s. The best fit to the observed data was obtained when r= 0.14 0.04 per cent and s= 26.0 2.7 per cent. Using these estimated values, predictions were made on the frequency of brown females and white males for up to 100 generations. The frequency of white males was predicted never to exceed 0.5 per cent, to be reached after 25 generations, while the frequency of brown females was expected to exceed 33 per cent after 25 generations. Published data on the mass reared T:Y(wp)30C strain, maintained with a population size of 240,000 adult flies, were subjected to the same analysis, indicating a higher value of s (38.0 3.2-52.0 0.3 per cent) under these conditions, but a similar value of recombination. Again, the frequency of white males was predicted never to exceed 0.5 per cent as a result of recombination. When analysing published data on T : Y( wp ÷ ) 101, another genetic sexing strain, a lower stability was indicated, i.e. a more rapid generation of brown females and white males than in T : Y( wp + )30C. No suitable values of s and r could be calculated from the T : Y( wp ÷ )101 data. It is concluded that outside contamination had probably occurred in the latter strain during both periods when it was mass reared.

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Section: Discussionmentioning

confidence: 84%

Section: Data On the Rates Of Breakdown Of T :Y(wp)10lmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

et al. 1993

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“…Rearing Mass rearing of T : Y(wp § )30C was initiated in January 1987, as described by Hooper (1987) with some minor modifications (Busch-Petersen & Kafu, 1989). A single oviposition cage, loaded with 4.5 1 of pupae, was maintained in each generation.…”

Section: Methodsmentioning

confidence: 99%

“…A genetic sexing strain, T : Y(wp § based on a pupal colour dimorphism, has been isolated in the medfly (Busch-Petersen et al, 1988), and the stability of the Y-linked translocation complex in this strain has been tested for 10 generations under mass-rearing conditions (Busch-Petersen & Kafu, 1989). Although a low level of aberrant female genotypes was observed, computer simulations by Hooper et aL (1987), extrapolated onto this data, suggested that this was caused by the accidental introduction of non-translocated karyotypes into the strain, rather than by a genetic instability in the strain itself (Busch-Petersen & Kafu, 1989). From the point of view of its genetic stability this strain thus appeared suitable for quality assessment under mass rearing conditions.…”

Section: Introductionmentioning

confidence: 99%

Assessment of quality control parameters during mass rearing of a pupal colour genetic sexing strain of the Mediterranean fruit fly

Busch-Petersen¹,

Kafu²

1989

Entomologia Exp Applicata

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Quality control charts were employed to monitor the quality of several parameters during mass rearing of the T: Y(WP +)30C strain of Ceratitis capitata (Wiedemann), in which male and female flies emerge from brown and white puparia, respectively. Egg hatch and pupal recovery in this genetic sexing strain were below that reported for a wild‐type ‘Sohag’ strain, whereas pupal size, adult emergence and flight ability were higher. The overall recovery from eggs to adult flies capable of flight was similar in the two strains but egg production in T: Y(wp +)30C was reduced to about 56% of that of ‘Sohag’. The quality control charts showed an acceptable level of variation within sets of data but the level between data sets was occasionally out of control. This indicated the presence of uncontrolled conditions during the rearing process, possibly arising from variations in the quality of the larval diet. It is concluded that the quality of T: Y(wp +)30C renders this strain suitable for mass rearing in sterile insect technique (SIT) programmes requiring the elimination of the female flies prior to the release of sterilized males. The next step should involve further quality assessments both under field cage and under field release conditions. The importance of optimal and standardized dietary conditions is stressed in relation to the quality of mass reared medflies. Résumé Bases du contrôle de l'état des Ceratitis capitata au cours d'un élevage de masse d'une lignée présentant un dimorphisme sexuel pupal Des normes de qualité ont été utilisées pour relever la valuer de plusieurs paramètres lors de l'élevage industriel de la souche T: Y(wp +)30C de C. capitata Wied. dont les mâles et les femelles émergent respectivement de pupes brunes et blanches. Les taux d'éclosion et d'obtention de pupes sont plus faibles avec cette souche qu'avec la souche souvage ‘Sohag’; par contre, la taille des pupes, le taux d'émergence des adultes et l'aptitude au vol étaient plus élevés. La fréquence totale des adultes capables de voler par rapport au nombre initial d'œufs a été de 0,297 contre environ 0,311 pour Sohag. Cependant, la production d'œufs a été réduite à environ 56% de celle de Sohag. Les tableaux de contrôle de la qualité ont révélé un niveau de variation acceptable à l'interieur des lots de données, mais la variabilité entre les lots excédait les normes admissibles. Ceci implique des paramètres incontrôlés pendant l'élevage de masse, probablement liés à des variations de la qualité de l'aliment. On peut en conclure que la qualité de la souche T: Y(wp +)30C la rend utilisable pour un élevage de masse dans des programmes d'utilisation d'insectes stériles (SIT) exigeant l'élimination des femelles avant le lâcher de mâles stériles. La prochaine étape impliquera de nouvelles épreuves de qualité dans des cages en champ et dans les conditions de libération dans la nature. L'importance des conditions optimales d'une alimentation standardisée est soulignée par la qualité des mouches obtenues en élevage de masse.

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Cytogenetic analysis of three genetic sexing strains of Ceratitits capitata

Kerremans¹,

Bourtzis

Zacharopoulou

1990

Theoret. Appl. Genetics

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Polytene chromosomes of three genetic sexing strains of Ceratitis capitata were analyzed. The genetic sexing mechanism is based on a pupal color dimorphism (white-brown) and is the result of a reciprocal translocation between the Y chromosome and the autosome bearing the w locus (white pupal case). The analyzed polytene chromosomes were derived from two different pupal tissues, the orbital bristle and fat body cells. The Y chromosome is visible in both tissues, while the autosomes present a different banding pattern. Based on these features, the autosome breakpoints in the three Y; autosome translocations were mapped, and the homology of the translocated autosome in both tissues was established. In addition, the location of the break-points was compared to the stability of these three strains.

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Stability of Two Mass-Reared Genetic Sexing Strains of Ceratitis capitata (Diptera: Tephritidae) Based on Pupal Color Dimorphisms

Cited by 17 publications

References 0 publications

Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

Assessment of quality control parameters during mass rearing of a pupal colour genetic sexing strain of the Mediterranean fruit fly

Cytogenetic analysis of three genetic sexing strains of Ceratitits capitata

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