Cytogenetic analysis of three genetic sexing strains of Ceratitits capitata

Kerremans, Ph.; Bourtzis, Kostas; Zacharopoulou, Antigone

doi:10.1007/bf00224383

Cited by 26 publications

(29 citation statements)

References 13 publications

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“…The higher value r of 0.14 0.04 per cent is expected to be the more accurate estimate (because fewer artificial contaminants were added) but, even so, it is lower than that observed by Busch-Petersen (1989) in strain T:Y(wp)101. Since Kerremans et al (1990) found the translocation breakpoints on the autosomes in T:Y(wp)30C and T:Y(wp)101 to be probably identical, the difference between our estimate of spontaneous recombination and that of Busch-Petersen (1989) may result from differences in experimental design. However, a difference in recombination rate would fit in with the observation that the T:Y(wp)30C sexing strain is more stable than the T:Y(wp)101 strain under mass rearing conditions (Busch-Petersen & Kafu, 1989).…”

Section: Discussioncontrasting

confidence: 57%

Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

et al. 1993

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The frequency of male recombination (r) between the translocation breakpoint and the wp + gene, and the selection coefficient (s) against wp/ wp, in the genetic sexing strain T : Y( wp + )30C of the Mediterranean fruit fly Ceratitis capitata (Wied.) were measured in population experiments over 11 generations. The population was 50 females and 50 males in the first generation, after which 150 pupae were used to start each subsequent generation. A computer program was used to estimate values of r and s. The best fit to the observed data was obtained when r= 0.14 0.04 per cent and s= 26.0 2.7 per cent. Using these estimated values, predictions were made on the frequency of brown females and white males for up to 100 generations. The frequency of white males was predicted never to exceed 0.5 per cent, to be reached after 25 generations, while the frequency of brown females was expected to exceed 33 per cent after 25 generations. Published data on the mass reared T:Y(wp)30C strain, maintained with a population size of 240,000 adult flies, were subjected to the same analysis, indicating a higher value of s (38.0 3.2-52.0 0.3 per cent) under these conditions, but a similar value of recombination. Again, the frequency of white males was predicted never to exceed 0.5 per cent as a result of recombination. When analysing published data on T : Y( wp ÷ ) 101, another genetic sexing strain, a lower stability was indicated, i.e. a more rapid generation of brown females and white males than in T : Y( wp + )30C. No suitable values of s and r could be calculated from the T : Y( wp ÷ )101 data. It is concluded that outside contamination had probably occurred in the latter strain during both periods when it was mass reared.

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Section: Discussioncontrasting

confidence: 57%

Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

et al. 1993

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“…The same structure is not seen in larval tissues such as salivary gland and fat body cells (Zacharopoulou 1987(Zacharopoulou , 1990, where the network is dispersed into small groups of granules. However, the sex chromosomes can be observed in pupal fat body cells (Kerremans et al 1990;A. Zacharopoulou, E.M. Riva, A. Ualaczida, and G. Gasperi, in preparation).…”

Section: Introductionmentioning

confidence: 78%

“…Trichogen cells contain a distinctive X/Y structure, made of a granular network, a compact dark sphere, and the nucleolus (Bedo 1987;Bedo and Zacharopoulou 1988). The same structure is not seen in larval tissues such as salivary gland and fat body cells (Zacharopoulou 1987(Zacharopoulou , 1990, where the network is dispersed into small groups of granules. However, the sex chromosomes can be observed in pupal fat body cells (Kerremans et al 1990;A.…”

Section: Introductionmentioning

confidence: 94%

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A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells in Ceratitis capitata

Zacharopoulou¹,

Bourtzis²,

Kerremans³

1991

Genome

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ZACHAROPOULOU, A., BOURTZIS, K., and KERREMANS, PH. 1991. A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells in Ceratitis capitata. Genome, 34: 215-219. The banding patterns of polytene chromosomes in different tissues of the Mediterranean fruit fly, Ceratitis capitata, vary to such an extent that homologous chromosomes cannot be recognised. However, analyses of autosomal breakpoints in several translocation strains allowed chromosomes from the two tissues to be aligned despite their difference in banding pattern. These results were discussed, considering the different hypotheses of the origin and biological significance of polytene chromosome bands. A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells in Ceratitis capitata. Genome, 34 : 215-219.Les profils de bandes des chromosomes polytknes de differents tissus de la mouche a fruit mediterraneenne, le Ceratitis capitata, varient a un point tel que les chromosomes homologues ne peuvent Etre distingues. Toutefois, des analyses des points de bris autosomiques chez plusieurs souches de translocation ont permis d'aligner les chromosomes de deux tissus, malgre leurs differences dans les profils de bandes. Ces resultats sont discutes a la lumikre d'hypothkses differentes sur l'origine et la signification biologique des bandes des chromosomes polytknes.

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“…Genetic sexing strains that allowed mass rearing of all-male populations for sterile release would be an advantage (Robinson and Van Heermert 1982;Hendrichs et al 1995). A number of genetic sexing strains have been constructed (reviewed in Robinson 1989;Kerremans et al 1990;Franz and Kerremans 1994), but these tend to break down under massrearing conditions. Large-scale field trials, however, have given encouraging results with a genetic sexing strain based on a T(Y;5) translocation carrying the wild-type allele of a temperature sensitive recessive lethal mutation that is homozygous in the female line (Willhoef et al 1996;Franz et al 1996;Redon et al 1997).…”

Section: Introductionmentioning

confidence: 98%

Recovery of a marked translocation strain that will facilitate the isolation of balancer chromosomes in the Mediterranean fruit fly,Ceratitis capitata

Gubb

Zacharopoulou²,

Livadaras³

et al. 1998

Genome

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The results of two screens for mutations and chromosomal aberrations in Ceratitis capitata are presented. Three dominant mutations were recovered, including Sb, which is associated with a homozygous lethal translocation between the third and fifth chromosomes, T(3;5)Sb, with the fifth chromosome breakpoint adjacent to y. The T(3;5)Sb chromosome is maintained by selecting for Sb in a T(3;5)Sb, w2 Sb y2 wp/w2 y2 wp stock and can be used to distinguish between other chromosomes carrying differential combinations of the recessive markers w2 y2 wp. The ability to isolate particular marked chromosomes is essential in order to recover an inversion-based balancer chromosome. In addition to the recovery of dominant mutations, gamma-ray induced somatic mosaics of w2 and y2 and zygotic w mosaics were found. The generation of zygotic mosaics following mutagenesis can give mutants with a mosaic germ line that fail to breed true in the first generation. A screen of 22,830 irradiated chromosomes failed to recover variegating alleles of w, although such alleles might be recovered in a larger screen. The high frequency of dominant mutations and the instability at the w locus in our stocks implies a background level of dysgenic activity. These results have implications for the construction and long-term maintenance of genetically modified strains.

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Cytogenetic analysis of three genetic sexing strains of Ceratitits capitata

Cited by 26 publications

References 13 publications

Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells in Ceratitis capitata

Recovery of a marked translocation strain that will facilitate the isolation of balancer chromosomes in the Mediterranean fruit fly,Ceratitis capitata

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