1991
DOI: 10.1139/g91-034
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A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells in Ceratitis capitata

Abstract: ZACHAROPOULOU, A., BOURTZIS, K., and KERREMANS, PH. 1991. A comparison of polytene chromosomes in salivary glands and orbital bristle trichogen cells in Ceratitis capitata. Genome, 34: 215-219. The banding patterns of polytene chromosomes in different tissues of the Mediterranean fruit fly, Ceratitis capitata, vary to such an extent that homologous chromosomes cannot be recognised. However, analyses of autosomal breakpoints in several translocation strains allowed chromosomes from the two tissues to be aligned… Show more

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Cited by 14 publications
(7 citation statements)
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“…In salivary gland nuclei, the X chromosome is represented by scattered groups of small granules but the Y chromosome cannot be observed in polytene chromosome slides prepared through routine protocols (Zacharopoulou, ; Zacharopoulou et al., ; Rosetto et al., ). The different banding patterns of polytene chromosomes in the two tissues may be attributed to the differential gene activity at different stages of development (Zacharopoulou, ; Zacharopoulou et al., ,b). Furthermore, the polytene chromosomes derived from salivary glands exhibit extensive ectopic pairing and breakage, contrary to the chromosomes of the trichogen cells.…”
Section: Ceratitis Capitata As a Model For Tephritidae: Historic Backmentioning
confidence: 99%
See 1 more Smart Citation
“…In salivary gland nuclei, the X chromosome is represented by scattered groups of small granules but the Y chromosome cannot be observed in polytene chromosome slides prepared through routine protocols (Zacharopoulou, ; Zacharopoulou et al., ; Rosetto et al., ). The different banding patterns of polytene chromosomes in the two tissues may be attributed to the differential gene activity at different stages of development (Zacharopoulou, ; Zacharopoulou et al., ,b). Furthermore, the polytene chromosomes derived from salivary glands exhibit extensive ectopic pairing and breakage, contrary to the chromosomes of the trichogen cells.…”
Section: Ceratitis Capitata As a Model For Tephritidae: Historic Backmentioning
confidence: 99%
“…After the correlation of polytene and mitotic chromosomes and following the numbering system established for mitotic chromosomes (Radu et al., ), the polytene elements were numbered 2–6 (Zacharopoulou, ). Moreover, the analysis of autosomal breakpoints in several translocation strains allowed the alignment of polytene chromosome maps from salivary gland and trichogen cells (Kerremans et al., ; Zacharopoulou et al., ,b). In addition, detailed comparative analysis was achieved by in situ hybridization with several cloned DNA sequences available for the C. capitata (Zacharopoulou et al., ; Kritikou, ; Gariou‐Papalexiou et al., ) (Tables and ).…”
Section: Ceratitis Capitata As a Model For Tephritidae: Historic Backmentioning
confidence: 99%
“…In contradiction to this, only five polytene chromosomes are found in salivary glands, corresponding to the five autosomes, whereas sex chromosomes do not form polytene elements. The X chromosome forms a granular network and the Y chromosome appears as a heterochromatic sphere in some polytene tissues (Bedo and Webb 1989;Zacharopoulou et al 1990).…”
Section: Resultsmentioning
confidence: 99%
“…Σύγκριση του χρωμοσωματικού προτύπου ζώνωσης στους δύο τύπους ιστών έδειξε σημαντικές διαφορές, με αποτέλεσμα να μην μπορούν να αναγνωριστούν ακόμη και τα ομόλογα χρωμοσώματα (Bedo and Zacharopoulou, 1988). Ετσι, η αντιστοιχία των χρωμοσωματικών χαρτών των σιελογόνων αδένων και των τριχογενών κυττάρων έγινε εφικτή με τη βοήθεια της κυτταρογενετικής ανάλυσης διαφόρων στελεχών που έφεραν Υ-αυτοσωματικές και αυτοσωματικές-αυτοσωματικές μετατοπίσεις (translocation strains), (Zacharopoulou et al, 1991a).…”
Section: μεσογειακη μυγα Ceratìtis Capitataunclassified
“…Αυτό βρέθηκε από τη μελέτη μίας σειράς Υ-αυτοσωματικών μετατοπίσεων (Bedo, 1987;Kerremans et al, 1990;Zacharopoulou et al, 1991b). Η παντελής απουσία ομολογίας μεταξύ των δύο τύπων πολυταινικών χρωμοσωμάτων (σιελογόνων αδένων και τριχογενών κυττάρων), ως προς το πρότυπο ζώνωσης των αυτοσωματικών χρωμοσωμάτων καθώς και ως προς τη μορφολογία των φυλετικών χρωμοσωμάτων μπορεί να οφείλεται, είτε στο διαφορετικό βαθμό πολυταινισμού στους δύο ιστούς ή/και στη διαφορετική γονιδιακή δραστηριότητα σ' αυτούς (Bedo, 1987;Zacharopoulou, 1990;Zacharopoulou et al, 1991a). H απουσία πολυταινισμού στα φυλετικά χρωμοσώματα των πολυταινικών πυρήνων στη Μεσογειακή μύγα συμφωνεί με τα κριτήρια της C-banding χρώσης των χρωμοσωμάτων, όπου και τα δύο, το Χ και το Υ χρωμόσωμα είναι ετεροχρωματινικά στους μιτωτικούς πυρήνες (Southern, 1976;Bedo, 1986).…”
Section: μεσογειακη μυγα Ceratìtis Capitataunclassified