Stability of the small γ-tubulin complex requires HCA66, a protein of the centrosome and the nucleolus

Fant, Xavier; Gnadt, Nicole; Haren, Laurence; Merdes, Andreas

doi:10.1242/jcs.035238

Cited by 24 publications

(32 citation statements)

References 47 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We discovered that, in the absence of Apaf1, recruitment of HCA66 to the centrosome is reduced (Fig. 9A,B,D); such a reduction has previously been reported as causing disorganized microtubule nucleation from centrosomes (Fant et al, 2009). Because HCA66 is an Apaf1 interactor, the lack of their binding might account for the dysregulation of HCA66 centrosomal localization we observed in the absence of Apaf1.…”

Section: Apaf1 Regulates Cytoskeleton Dynamics 3459supporting

confidence: 67%

“…Strikingly, one of the known Apaf1 interactors, HCA66, has been found very recently to be localized into centrosomes (Fant et al, 2009).…”

Section: Apaf1-deficient Cells Are Defective In Cell Migrationmentioning

confidence: 99%

“…c-Tubulin moves from the cytosol to the centrosomes, the recruitment of c-tubulin to the centrosomes being regulated by kinases, by proteins of the pericentriolar material and by a protein binding the c-TuRC called NEDD1 (neural precursor cell expressed, developmentally downregulated 1) (Haren et al, 2006;Luders et al, 2006;Oegema et al, 1999). Recently it has been found that the hepatocellular carcinoma antigen 66 (HCA66), which had been previously described as an interactor of Apaf1 and a positive regulator of Apaf1-dependent apoptosis (Piddubnyak et al, 2007), is required for the stability of c-TuSC proteins and that its deficiency affects spindle microtubule assembly (Fant et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Apaf1 plays a pro-survival role by regulating centrosome morphology and function

Ferraro

Pesaresi

Zio

et al. 2011

Journal of Cell Science

View full text Add to dashboard Cite

SummaryThe apoptotic protease activating factor 1 (Apaf1) is the main component of the apoptosome, and a crucial factor in the mitochondriadependent death pathway. Here we show that Apaf1 plays a role in regulating centrosome maturation. By analyzing Apaf1-depleted cells, we have found that Apaf1 loss induces centrosome defects that impair centrosomal microtubule nucleation and cytoskeleton organization. This, in turn, affects several cellular processes such as mitotic spindle formation, cell migration and mitochondrial network regulation. As a consequence, Apaf1-depleted cells are more fragile and have a lower threshold to stress than wild-type cells. In fact, we found that they exhibit low Bcl-2 and Bcl-X L expression and, under apoptotic treatment, rapidly release cytochrome c. We also show that Apaf1 acts by regulating the recruitment of HCA66, with which it interacts, to the centrosome. This function of Apaf1 is carried out during the cell life and is not related to its apoptotic role. Therefore, Apaf1 might also be considered a pro-survival molecule, whose absence impairs cell performance and causes a higher responsiveness to stressful conditions.

show abstract

Section: Apaf1 Regulates Cytoskeleton Dynamics 3459supporting

confidence: 67%

“…Strikingly, one of the known Apaf1 interactors, HCA66, has been found very recently to be localized into centrosomes (Fant et al, 2009).…”

Section: Apaf1-deficient Cells Are Defective In Cell Migrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Apaf1 plays a pro-survival role by regulating centrosome morphology and function

Ferraro

Pesaresi

Zio

et al. 2011

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…The origin of a comet on a kymographed microtubule marks the site from which the microtubule evidently grows, and in these Arabidopsis plants expressing AtEB1-GFP under the 35S promoter, that site is marked by a focus of EB1. In animal cells, neural precursor cell expressed developmentally downregulated protein 1 (NEDD1), targets the gamma tubulin ring complex to the centrosome (Fant et al, 2009). In plants, NEDD1 decorates the spindle poles of plant cells (Zeng et al, 2009) and during interphase labels cytoplasmic spots associated with cortical microtubules (Motose et al, 2008).…”

Section: The Emergence Of Cortical Microtubulesmentioning

confidence: 99%

ArabidopsisCortical Microtubules Are Initiated along, as Well as Branching from, Existing Microtubules

et al. 2009

View full text Add to dashboard Cite

The principles by which cortical microtubules self-organize into a global template hold important implications for cell wall patterning. Microtubules move along bundles of microtubules, and neighboring bundles tend to form mobile domains that flow in a common direction. The bundles themselves move slowly and for longer than the individual microtubules, with domains describing slow rotary patterns. Despite this tendency for colinearity, microtubules have been seen to branch off extant microtubules at ;458. To examine this paradoxical behavior, we investigated whether some microtubules may be born on and grow along extant microtubule(s). The plus-end markers Arabidopsis thaliana end binding protein 1a, AtEB1a-GFP, and Arabidopsis SPIRAL1, SPR1-GFP, allowed microtubules of known polarity to be distinguished from underlying microtubules. This showed that the majority of microtubules do branch but in a direction heavily biased toward the plus end of the mother microtubule: few grow backward, consistent with the common polarity of domains. However, we also found that a significant proportion of emergent comets do follow the axes of extant microtubules, both at sites of apparent microtubule nucleation and at cross-over points. These phenomena help explain the persistence of bundles and counterbalance the tendency to branch.

show abstract

“…In 2009, Apaf1 was also proven to interact with the centrosomal protein hepatocellular carcinoma antigen 66 (HCA66) required for the stability of the small g-tubulin complex (g-TuSC) (Fant et al, 2009). g-tubulin is one component of the pericentriolar material in the centrosome, promoting the nucleation of microtubule polymers from a-b-tubulin dimers.…”

Section: Apaf1 and The Centrosomementioning

confidence: 99%

Apaf1 in embryonic development - shaping life by death, and more

2015

View full text Add to dashboard Cite

Apaf1 has been studied hitherto for its key role in regulating the formation of the apoptotic core machinery, the apoptosome, to induce programmed cell death. Apaf1 involvement in orchestrating this process during embryonic development has been widely documented and constitutes a breakthrough in developmental biology. In this review, we aim to highlight the origin of Apaf1 discoveries and how findings, mainly based on the analysis of knock-out mouse models, have led us to consider Apaf1 as a master player in fine-tuning apoptosis during embryonic development. Likewise, we also attempt to establish how Apaf1 function is locally time-dependent in regulating neurodevelopment and becomes dispensable during neuron maturation. We go on to discuss Apaf1's new functions which have been unveiled in recent years and which could revise or, at least, adjust the common view of Apaf1 having merely an apoptotic role. Hence, by presenting clear indications on the pro-survival roles of Apaf1, this review seeks to provide novel and more complex insights into Apaf1 involvement in nervous system development.

show abstract

Stability of the small γ-tubulin complex requires HCA66, a protein of the centrosome and the nucleolus

Abstract: SummaryStability of the small γ-tubulin complex requires HCA66, a protein of the centrosome and the nucleolus

Cited by 24 publications

References 47 publications

Apaf1 plays a pro-survival role by regulating centrosome morphology and function

Apaf1 plays a pro-survival role by regulating centrosome morphology and function

ArabidopsisCortical Microtubules Are Initiated along, as Well as Branching from, Existing Microtubules

Apaf1 in embryonic development - shaping life by death, and more

Contact Info

Product

Resources

About