Apaf1 in embryonic development - shaping life by death, and more

Zio, Daniela De; Maiani, Emiliano; Cecconi, Francesco

doi:10.1387/ijdb.150047dd

Cited by 8 publications

(9 citation statements)

References 55 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistently, apart from its canonical role in cell death, it was recently shown that Apaf1 is necessary for other cellular processes, including mitosis, migration, synaptogenesis, and neuronal survival (Ferraro et al, ). For example, neurons lacking Apaf1 exhibit impaired axonal outgrowth, caused by abnormal centrosome organization and alteration of signaling molecules important for axonal extension, including the glycogen synthase kinase‐3b and collapsing‐response mediator protein‐2 (De Zio, Maiani, & Cecconi, ). Apaf1‐deficient mice show hyper proliferation of embryonic NSPCs and decreased apoptosis in the brain, resulting in perinatal lethality (H. Yoshida et al, ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system

Yamada

Sakakibara

2018

J of Comparative Neurology

View full text Add to dashboard Cite

The orchestrated events required during brain development, as well as the maintenance of adult neuronal plasticity, highly depend on the accurate responses of neuronal cells to various cellular stress or environmental stimuli. Recent studies have defined a previously unrecognized, broad class of multidomain proteins, designated as signal transduction ATPases with numerous domains (STAND), which comprises a large number of proteins, including the apoptotic peptidase activating factor 1 (Apaf1) and nucleotide-binding oligomerization domain-like receptors (NLRs), central players in cell death and innate immune responses, respectively. Although the involvement of STANDs in the central nervous system (CNS) has been postulated in terms of neuronal development and function, it remains largely unclear. Here, we identified Nwd1 (NACHT and WD repeat domain-containing protein 1), as a novel STAND protein, expressed in neural stem/progenitor cells (NSPCs). Structurally, Nwd1 was most analogous to the apoptosis regulator Apaf1, also involved in mitosis and axonal outgrowth regulation in the CNS. Using a specific antibody, we show that, during the embryonic and postnatal period, Nwd1 is expressed in nestin-positive NSPCs in vivo and in vitro, while postnatally it is found in terminally differentiated neurons and blood vessels. At the subcellular level, we demonstrate that Nwd1 is preferentially located in the cytosolic compartment of cultured NSPCs, partially overlapping with cytochrome c. These observations imply that Nwd1 might be involved in the neuronal lineage as a new STAND gene, including having a pro-apoptotic or nonapoptotic role, similar to Apaf1.

show abstract

Section: Discussionmentioning

confidence: 99%

“…and collapsing-response mediator protein-2 (De Zio, Maiani, & Cecconi, 2015). Apaf1-deficient mice show hyper proliferation of embryonic NSPCs and decreased apoptosis in the brain, resulting in perinatal lethality (H. Yoshida et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system

Yamada

Sakakibara

2018

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…APAF1 serves a critical role as a regulator of the mitochondrial apoptotic signaling pathway, inducing cell apoptosis by binding with cytochrome C and activating caspase-9 in the cytosol ( 21 ). Previous studies have demonstrated that APAF1 functions as a tumor suppressor by interacting with various miRNAs ( 40 – 42 ).…”

Section: Discussionmentioning

confidence: 99%

“…Apoptotic protease activating factor 1 (APAF1) is a critical component of the apoptosome and previous studies have demonstrated that it may be activated by various cellular stimuli, including DNA damage and oncogene activation ( 20 , 21 ). APAF1 inactivation is a common event in human tumors, which suggests that it may serve as a tumor suppressor in healthy individuals ( 22 ).…”

Section: Introductionmentioning

confidence: 99%

miR‑186 promotes tumor growth in cutaneous squamous cell carcinoma by inhibiting apoptotic protease activating factor‑1

Tian

Shen

Yan³

et al. 2018

Exp Ther Med

View full text Add to dashboard Cite

Cutaneous squamous cell carcinoma (cSCC) accounts for 20% of non-melanoma skin cancer worldwide. MicroRNAs (miRNAs or miRs) are a subtype of non-coding RNA associated with the progression of various types of human cancer. MiR-186 has been demonstrated to act as an oncogene in human tumors. However, the role of miR-186 in cSCC remains unclear. The expression of miR-186 and apoptotic protease activating factor 1 (APAF1) was examined using reverse transcription-quantitative polymerase chain reaction, western blotting and immunofluorescence. The correlation between miR-186 and APAF1 was determined using a dual-luciferase assay. Mimics or inhibitors of miR-186 were transfected into A-431 cells to establish cell lines with overexpressed or knocked-down miR-186, respectively. EdU staining and colony formation assays were performed to detect cell proliferation. Transwell and wound-healing assays were performed to analyze cell invasion and migration, respectively. Hoechst staining and flow cytometry were performed to assess cell apoptosis and cell cycle distribution. MiR-186 expression was significantly increased, while APAF1 expression was significantly decreased in cSCC tissues compared with the controls. An miR-186 binding site was predicted in APAF1 and their expression was negatively correlated in cSCC tissues. Cell proliferation, invasion and migration were significantly enhanced in the miR-186-overexpressed A-431 cells and attenuated in miR-186 knockdown cells compared with the control. APAF1 expression was regulated by miR-186, while APAF1 knockdown significantly promoted cell invasion and inhibited cell apoptosis. In summary, the results of the present study indicate that miR-186 serves as an oncogene in cSCC by inhibiting APAF1.

show abstract

“…PMAIP1 is a gene which promotes activation of caspases and apoptosis by altering the mitochondrial membrane and efflux of apoptogenic proteins. Apoptotic peptidase activating factor 1 (Apaf-1) is an important regulator of the mitochondrial apoptotic pathway to induce programmed cell death [ 37 , 38 ]. In the present study, Bcl-2 , an anti-apoptotic member of the Bcl-2 family, was up-regulated.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus

et al. 2017

View full text Add to dashboard Cite

Orf virus has been utilized as a safe and efficient viral vector against not only diverse infectious diseases, but also against tumors. However, the nature of the genes triggered by the vector in human cells is poorly characterized. Using RNA sequencing technology, we compared specific changes in the transcriptomic profiles in human foreskin fibroblast cells following infection by the orf virus. The results indicated that orf virus upregulates or downregulates expression of a variety of genes, including genes involved in antiviral immune response, apoptosis, cell cycle and a series of signaling pathways, such as the IFN and p53-signaling pathways. The orf virus stimulates or inhibits immune gene expression such as chemokines, chemokine receptors, cytokines, cytokine receptors, and molecules involved in antigen uptake and processing after infection. Expression of pro-apoptotic genes increased at 8 hours post-infection. The p53 signaling pathway was activated to induce apoptosis at the same time. However, the cell cycle program was promoted after infection, which may be due to the immunomodulatory genes of the orf virus. This presents the first description of transcription profile changes in human foreskin fibroblast cells after orf virus infection and provides an in-depth analysis of the interaction between the host and orf virus. These data offer new insights into the understanding of the mechanisms of infection by orf virus and identify potential targets for future studies.

show abstract

Apaf1 in embryonic development - shaping life by death, and more

Cited by 8 publications

References 55 publications

Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system

Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system

miR‑186 promotes tumor growth in cutaneous squamous cell carcinoma by inhibiting apoptotic protease activating factor‑1

Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus

Contact Info

Product

Resources

About