Stability of the CK-MB isoenzyme on routine storage

Buttery, J E; Stuart, Sandra; Pannall, P R

doi:10.1016/0009-9120(92)80039-j

Cited by 11 publications

(5 citation statements)

References 7 publications

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“…The stability of CK-MB mass and troponin I have been previously studied (2)(3)(4)(5) but not the stability of troponin T. There has been no study using Arrhenius plots to effectively calculate stability across a range of temperatures. We show that troponin T, troponin I and CK-MB mass all show a rapid decrease in stability between 15 and 20° C. At this temperature, a common environmental temperature encountered within laboratories, maximum diagnostic information will only be obtained if laboratories take this instability into account in their standard operating procedures.…”

Section: Discussionmentioning

confidence: 99%

Stability of Cardiac Troponins and CK-MB Mass Isoenzyme

Price¹,

Burgin²,

Cruise³

2000

Clinical Chemistry and Laboratory Medicine

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The stability of an analyte can have an important influence on its diagnostic performance and therefore should be documented before an analyte is introduced into routine practice. The stability of creatine kinase MB isoenzyme (CK-MB), troponin I and troponin T was studied by incubating samples at temperatures between 26 degrees C and 39 degrees C for periods up to 5400 min. Arrhenius plots were made which showed that all three proteins demonstrated a rapid decrease in stability between 15 degrees C and 20 degrees C. The activation energies for CK-MB, troponin I and troponin T were 7.7, 6.34 and 6.34 cal/mol, respectively.

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Section: Discussionmentioning

confidence: 99%

Stability of Cardiac Troponins and CK-MB Mass Isoenzyme

Price¹,

Burgin²,

Cruise³

2000

Clinical Chemistry and Laboratory Medicine

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“…RF ablation of more myocardial tissue in the atria, such as in PV isolation, is expected to have much higher cTn levels [10][11][12][20][21][22][23][24][25]. Previous clinical studies reported contrasting data, in the setting of AF ablation, on cardiac biomarkers levels after cryoballoon ablation compared to RF ablation [7,[13][14][15][16][17][18][19]26]. Kuhne et al [7] reported higher post procedural cTnT levels in RF group vs. cryoballoon group.…”

Section: Previous Studiesmentioning

confidence: 99%

“…As several markers have been proven to be useful for diagnosis and evaluation of size of myocardial injury after acute ischemic episode, many authors tried to implement different biomarkers to evaluate and quantify the size of effective ablation lesions [5][6][7][8][9][10][11][12][13][14][15][16][17][18]. On one hand, previous studies have univocally shown humoral biomarkers for myocardial injury to be elevated after radiofrequency (RF) ablation, depending on the amount of energy delivered and the site of ablation [5].…”

Section: Introductionmentioning

confidence: 99%

Biomarkers of myocardial injury with different energy sources for atrial fibrillation catheter ablation

Casella

Russo

et al. 2014

Cardiol J

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“…Specimens for CK analysis should be processed quickly and stored well-sealed and away from light. Enzyme activity in samples from some species can degrade significantly over a few hours if samples are stored at room temperature, in bright light or in loosely capped tubes (Buttery et al, 1992). Creatine kinase total and isoenzyme activity is stable is samples subjected to short-term refrigeration or freezing, although some laboratories evaluating isozymes may prefer fresh samples to avoid any possible differential isoenzyme degradation with storage (Preus et al, 1989; Ercan and Grossman, 2003).…”

Section: Enzymes Of Muscle Injurymentioning

confidence: 99%

Serum Chemical Biomarkers of Cardiac Injury for Nonclinical Safety Testing

Walker¹

2006

Toxicol Pathol

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There has been a substantial increase in the number and efficacy of laboratory biomarkers for the evaluation of human cardiac injury over the last decade. The advantages of these over traditional laboratory tests have encouraged adoption of comparable markers in nonclinical studies for cardiac injury assessment. Of particular interest are markers that are not only more sensitive and/or specific than traditional parameters for cardiac injury, but also those that may directly bridge human and laboratory animal safety testing. However, a majority of recently developed markers are quantified through antibody-based assays, and cross-reactivity with the comparable analyte in nonhuman samples is difficult to predict and often species-variable. The utility of these novel biomarkers thus, depends upon adequate assay validation with each laboratory species of interest. In contrast, traditional laboratory parameters of cardiac injury lack the properties of an ideal biomarker, but are well established and have an extensive database in nonclinical safety assessment. The current status and utility of both recently developed and traditional biomarkers of cardiac injury in nonclinical testing, and considerations for validation of novel biomarkers of cardiac injury are reviewed.

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Stability of the CK-MB isoenzyme on routine storage

Cited by 11 publications

References 7 publications

Stability of Cardiac Troponins and CK-MB Mass Isoenzyme

Stability of Cardiac Troponins and CK-MB Mass Isoenzyme

Biomarkers of myocardial injury with different energy sources for atrial fibrillation catheter ablation

Serum Chemical Biomarkers of Cardiac Injury for Nonclinical Safety Testing

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