Stability of Cardiac Troponins and CK-MB Mass Isoenzyme

Price, A.; Burgin, Christine; Cruise, Martin

doi:10.1515/cclm.2000.177

Cited by 1 publication

(2 citation statements)

References 5 publications

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“…We detected a significant decrease in cTnI recovery when cTnI samples were stored at room temperature and at 4 °C for 2 days. These findings are similar to previous studies in laboratory animals and humans 19,38–40 . Storage at −20 °C for 7 days had no significant effect on cTnI recovery.…”

Section: Discussionsupporting

confidence: 92%

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Validation of a Commercially Available Immunoassay for the Measurement of Bovine Cardiac Troponin I

Varga

Schober

Walker

et al. 2009

Veterinary Internal Medicne

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Background: Commercially available cardiac troponin I (cTnI) assays developed for use in humans have not yet been validated for use in cattle.Hypotheses: The ADVIA Centaur TnI-Ultra immunoassay can be used for the detection of bovine cTnI. In healthy cattle, serum cTnI is undetectable or is present only in trace amounts.Methods: Purified bovine cTnI and cTnI-free bovine serum were used for the evaluation of assay performance including intra-and inter-assay precision, sensitivity, interference, linearity, and recovery. Effects of storage at 23, 4, À20, and À80 1C for 2 days, and at À20 and À80 1C for 7 and 14 days and repeated freeze-thaw cycles on recovery of cTnI were analyzed. Serum cTnI concentrations in 30 healthy dairy cows were determined.Results: Intra-and inter-assay precisions (mean AE SD) were 4.48 AE 2.26 and 13.36 AE 6.59%, respectively. The assay demonstrated linearity at 0.5, 2, 15, and 30 ng/mL cTnI. Mean recovery was 100.81, 85.26, 87.72, and 114.42%, respectively. Skeletal muscle homogenate added to serum of known cTnI concentration did not alter the concentration of the analyte (P 4 .05). Concentration of cTnI significantly decreased when samples were stored at 4 and 23 1C for 2 days (P o .05). Repeated freeze-thaw cycles and storage at À20 1C for 7 days had no significant influence on cTnI concentration (P 4 .05). Serum cTnI concentration in healthy cattle was 0.03 ng/mL.Conclusion and Clinical Importance: ADVIA Centaur can be used reliably for the detection of serum cTnI concentration in cattle.

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Section: Discussionsupporting

confidence: 92%

“…These findings are similar to previous studies in laboratory animals and humans. 19,[38][39][40] Storage at À20 1C for 7 days had no significant effect on cTnI recovery. In contrast, a significant, although possibly clinically irrelevant, decrease in cTnI recovery was noticed after storage for 2 days at À80 1C.…”

Section: Discussionmentioning

confidence: 85%