Stability of nitroglycerin in human and rat plasma

Maier, Gary; Poliszczuk, A.; Fung, Ho‐Leung

doi:10.1016/0378-5173(79)90100-5

Cited by 14 publications

(1 citation statement)

References 13 publications

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“…This should be taken into account when glyceryl trinitrate plasma concentrations are determined; blood should be collected in chilled tubes and immediately centrifuged at 4°C, and the extraction procedure should start as soon as possible. It is uncertain whether the tubes used for collecting blood should contain the enzyme inhibitor iodoacetamide (Cossum & Roberts 1985) or whether silver nitrate should be used to minimise loss when plasma is stored at -20°C before analysis (Maier et al 1979). Care should also be taken to avoid adsorption to polyvinyl chloride catheters or tubes during the sampling and other procedures.…”

Section: Assay Methodsmentioning

confidence: 99%

Clinical Pharmacokinetics of Glyceryl Trinitrate Following the Use of Systemic and Topical Preparations

Bogaert

1987

Clinical Pharmacokinetics

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Glyceryl trinitrate has been used for more than a century for the treatment of angina pectoris and, more recently, for the treatment of congestive heart failure. The introduction of transdermal delivery systems has renewed the controversy regarding the efficacy of the drug, mainly in the light of the development of tolerance. With concentrations of the order of 1 microgram/L or less, the measurement of glyceryl trinitrate in plasma is not easy: gas chromatography with electron capture detection has been used widely but recently gas chromatography-mass spectrometry has provided satisfactory results. Assay problems are most likely to be responsible for some of the unexpected results reported. Further factors which may confound the results of the study of plasma concentrations are the rapid metabolism of glyceryl trinitrate in blood in vitro, adsorption to containers and infusion sets, and the uptake and/or metabolism in vessel walls. From the intravenous infusion data, the large interindividual variability in plasma concentrations of glyceryl trinitrate is apparent. The plasma half-life is about 2 to 3 minutes; plasma clearance values reported vary from 216 to 3270 L/h, indicating extensive non-hepatic metabolism. With transdermal administration, mainly with the transdermal controlled delivery systems, plasma concentrations of glyceryl trinitrate appear to be maintained for up to 24 hours, with large interindividual variations. Despite the ability to maintain, for example with the transdermal delivery systems, relatively constant concentrations of glyceryl trinitrate, it has not been possible to find a relationship between plasma concentrations and pharmacological or clinical effects. This is in part due to the attenuation of the effects with time; from the available data it is clear that this attenuation occurs at a pharmacodynamic level (reflex adaptation and tolerance) and not at the pharmacokinetic level.

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Section: Assay Methodsmentioning

confidence: 99%