Stability of invertase in alcoholysis reactions with methanol

Rodríguez, Mabel; Gómez, A.; González, F.; Bárzana, Eduardo; López-Munguı́a, Agustı́n

doi:10.1016/s1381-1177(96)00063-x

Cited by 10 publications

(10 citation statements)

References 10 publications

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“…This SDS-PAGE profile is identical with the previously published profile for the preparation of total external invertase (Trimble & Maley, 1977;Rodriguez, Gomez, Gonzalez, Barzana, & Lopez-Munguia, 1997). The diffuse band in the SDS-PAGE is characteristic for external invertase and glycoproteins in general.…”

Section: Electrophoretic Analysis Of External Invertase Isoformssupporting

confidence: 87%

“…It has been previously published that deactivation of the external invertase follows biphasic kinetic (Cavaille & Combes, 1995;Rodriguez et al, 1997;Vrabel, Polakovič, Štefuca, & Baleš, 1997). Our obtained results implied that the cause of biphasic kinetics of denaturation could be caused by the existence of the four isoforms in the preparations of external invertase.…”

Section: Thermal Stabilitysupporting

confidence: 66%

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Purification and characterisation of Saccharomyces cerevisiae external invertase isoforms

2010

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Section: Electrophoretic Analysis Of External Invertase Isoformssupporting

confidence: 87%

Section: Thermal Stabilitysupporting

confidence: 66%

Purification and characterisation of Saccharomyces cerevisiae external invertase isoforms

2010

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“…The decreasing enzyme activity of the CWI was observed just in the highest concentration of methanol (50%). Higher half life of whole cell derivate with invertase activity then soluble in methanol solution was reported previously (47), but stability studies with free and covalent immobilized soluble invertase showed that immobilization does not favor enzyme stability in the presence of methanol (48).…”

mentioning

confidence: 66%

“…Nevertheless, there is a reaction which includes usage of invertase in alcohol solutions (47). Invertase is capable of transferring fructose to water (hydrolysis) and to alcohols (alcoholysis).…”

mentioning

confidence: 99%

Comparative study of stability of soluble and cell wall invertase fromSaccharomyces cerevisiae

Margetić

Vujčić

2016

Preparative Biochemistry & Biotechnology

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Yeast Saccharomyces cerevisiae is the most significant source of enzyme invertase. It is mainly used in the food industry as a soluble or immobilized enzyme. The greatest amount of invertase is located in the periplasmic space in yeast. In this work, it was isolated into two forms of enzyme from yeast S. cerevisiae cell, soluble and cell wall invertase (CWI). Both forms of enzyme showed same temperature optimum (60°C), similar pH optimum, and kinetic parameters. The significant difference between these biocatalysts was observed in their thermal stability, stability in urea and methanol solution. At 60°C, CWI had 1.7 times longer half-life than soluble enzyme, while at 70°C CWI showed 8.7 times longer half-life than soluble enzyme. After 2-hr of incubation in 8 M urea solution, soluble invertase and CWI retained 10 and 60% of its initial activity, respectively. During 22 hr of incubation of both enzymes in 30 and 40% methanol, soluble invertase was completely inactivated, while CWI changed its activity within the experimental error. Therefore, soluble invertase and CWI have not shown any substantial difference, but CWI showed better thermal stability and stability in some of the typical protein-denaturing agents.

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“…, Rodríguez et al . ) whose activity might change sugar compositions, and also to inhibit microbial growth (Shenoy et al . ).…”

Section: Relative Percent Compositions and Concentrations Of Sugars Amentioning

confidence: 99%

Foliar Extrafloral Nectar of Humboldtia brunonis (Fabaceae), a Paleotropic Ant‐plant, is Richer than Phloem Sap and More Attractive than Honeydew

et al. 2014

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Stability of invertase in alcoholysis reactions with methanol

Cited by 10 publications

References 10 publications

Purification and characterisation of Saccharomyces cerevisiae external invertase isoforms

Purification and characterisation of Saccharomyces cerevisiae external invertase isoforms

Comparative study of stability of soluble and cell wall invertase fromSaccharomyces cerevisiae

Foliar Extrafloral Nectar of Humboldtia brunonis (Fabaceae), a Paleotropic Ant‐plant, is Richer than Phloem Sap and More Attractive than Honeydew

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