Purification and characterisation of Saccharomyces cerevisiae external invertase isoforms

Andjelković, Uroš; Pićurić, Srdjan; Vujčić, Zoran

doi:10.1016/j.foodchem.2009.11.013

Cited by 53 publications

(66 citation statements)

References 25 publications

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“…Sanjay and Sugunan (2006) reported maximum activity at pH 5 and at 50°C. Andjelkovic et al (2010) reported as 3.5- 5.0 the optimum pH. The optimum pH from 3.5 to 7.0 hasbeen reported for invertases isolated from different yeasts (Belcarz et al , 2002; Persike et al, 2002).…”

Section: Resultsmentioning

confidence: 96%

“…The optimum pH from 3.5 to 7.0 hasbeen reported for invertases isolated from different yeasts (Belcarz et al , 2002; Persike et al, 2002). Andjelkovic et al 2010) reported as 60°C as optimum temperature of invertase action. In general, invertases show high activity in the temperature range of 35–75°C depending to their sources andalso incubation time (Persike et al, 2002; Kern et al, 1992).…”

Section: Resultsmentioning

confidence: 98%

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Stimulation of extracellular invertase production from spent yeast when sugarcane pressmud used as substrate through solid state fermentation

Kumar¹,

Kesavapillai²

2012

SpringerPlus

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Efforts were made to utilize the waste/by-product of two agro-process industries namely pressmud from sugar processing industries and spent yeast from distilleries manufacturing ethanol from cane molasses, for the production of microbial invertase. Our experimentation indicated that these two sources could be ideally utilized for the production of invertase through solid substrate fermentation (SSF). SSF with spent yeast had given highest specific activity of 430 U/mg in 72 h of fermentation. Inoculum percentage of yeast cells on pressmud was optimized as 50% (w/w) with a combination inoculum of spent yeast and fresh cultured yeast at a ratio of 7:3. Crude enzyme was characterized for optimum pH and temperature and maximum activity was recorded at pH 5.0 and at a temperature of 40°C. Impacts of metal ions and detergents on invertase action were studied in which Mn2+, Fe3+, Al3+ and detergents had enhanced the activity of the enzyme whereas Cu2+ and Zn2+ inhibited the enzyme activity. Purification of 9.8 folds was obtained by using three phase partition method.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 98%

Stimulation of extracellular invertase production from spent yeast when sugarcane pressmud used as substrate through solid state fermentation

Kumar¹,

Kesavapillai²

2012

SpringerPlus

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“…Intriguingly, no invertase activity was detected on cell free supernatant. Such phenomena could be explained by the fact that eventual secreted invertase could be retained on periplasmatic space of fungal cell, avoiding its diffusion to the liquid phase, as described to S. cerevisiae (Andjelkovic et al, ).…”

Section: Resultsmentioning

confidence: 99%

Purification and characterization of an invertase and a transfructosylase from Aspergillus terreus

et al. 2018

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Two intracellular invertases from Aspergillus terreus were purified and characterized. Invertase 1 has been shown maximal activity at pH 3.0 and optimum temperature at 60°C, while invertase 2 presented maximal activity at pH 4.6 and optimum temperature at 55°C. Km of invertase 1 and 2 for sucrose were 9.8 and 6.2 mM, respectively. Invertase 2 showed high thermostability presenting half‐life of 44, 14.9, and 2 hr at 40, 55, and 60°C, respectively. Gel filtration and SDS‐PAGE analysis revealed that invertase 2 has a dimeric structure composed by two monomers of 32 kDa. Fructose was a competitive inhibitor for invertase 2 presenting Ki of 61.5 mM. Besides hydrolytic activity, invertase 2 also showed transfructosylating activity producing fructooligosaccharides. The enzymes have potential to be applied in food industry since its product, inverted sugar, is used in candies and syrup production, while fructooligosacharides are prebiotics, low calorie and noncariogenic sweeteners. Practical applications Enzymes invertases hydrolyze sucrose in glucose and fructose producing inverted sugar. This product is widely used in food industry to produce jams, syrups, and fondant fillings for chocolates. Besides hydrolysis activity, some invertases can also present transfructosylating activity producing fructooligosaccharides. These oligosaccharides can act as prebiotic, providing beneficial health effects and can be used as low calorie and noncariogenic sweeteners. Aspergillus terreus produced two intracellular enzymes, one exclusively invertase, and the other, invertase and transfructosylase. The enzymes were purified and characterized in this study. This is an important step on screening new enzymes able to produce inverted sugar and fructooligosaccharides from sucrose.

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“…This mixture is commonly known as invert syrup and is widely used in the food and beverage industries because it is sweeter than glucose. This allows a higher degree of sweetness in a product without crystallization . Invertase is also used in the production of artificial honey, as a plasticizing agent in cosmetics, and in the pharmaceutical and paper industries …”

Section: Introductionmentioning

confidence: 99%

Monte Carlo economic analysis of Baker's yeast invertase purification using two‐ and three‐phase partitioning

Torres‐Acosta

Morales‐Guzman

Ruiz‐Ruiz

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two‐phase systems (ATPS) and three‐phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t‐butanol hinders its acceptance. CONCLUSION Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs. © 2018 Society of Chemical Industry

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Purification and characterisation of Saccharomyces cerevisiae external invertase isoforms

Cited by 53 publications

References 25 publications

Stimulation of extracellular invertase production from spent yeast when sugarcane pressmud used as substrate through solid state fermentation

Stimulation of extracellular invertase production from spent yeast when sugarcane pressmud used as substrate through solid state fermentation

Purification and characterization of an invertase and a transfructosylase from Aspergillus terreus

Monte Carlo economic analysis of Baker's yeast invertase purification using two‐ and three‐phase partitioning

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