Stability of hematological analytes depends on the hematology analyser used: A stability study with Bayer Advia 120, Beckman Coulter LH 750 and Sysmex XE 2100

Imeri, Fatime; Herklotz, Roberto; Risch, Lorenz; Arbetsleitner, Christine; Zerlauth, M; Risch, G.; Huber, Andreas

doi:10.1016/j.cca.2008.07.018

Cited by 86 publications

(105 citation statements)

References 10 publications

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“…Acceptable stability of the analytes was expected because the collections were stored at a controlled temperature and differential counting of leukocytes was not addressed in this study. 14,22 Third, the analyses were performed to obtain cell counts to select high-quality collections for processing and thus, a relatively simple hematology analyzer was applied. More advanced instrumentation may yield additional information on the observed relationships between, for example, nucleated RBCs and other cell counts.…”

Section: Discussionmentioning

confidence: 99%

“…11 Typically, the type of cell analyzer and its principles of measurement are given in association with the results for cellular characteristics because the results are, to some extent, depending on the principles and software used in the analysis. [12][13][14] Some measurements are independent of each other, such as hemoglobin (Hb) concentration and cell counts other than red blood cells (RBCs). Relationships between CB cellular characteristics other than white blood cell (WBC) subpopulations have been rarely described.…”

Section: Introductionmentioning

confidence: 99%

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Association of cord blood platelet count and volume with hemoglobin in healthy term infants

et al. 2010

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Objective: The aim of this study was to investigate relationships of cord blood cells in healthy term infants both from vaginal and Cesarean sections.Study Design: The study sample comprised 167 consecutive cord blood collections accepted for processing in an accredited cord blood bank. The effect of varying anticoagulant-to-blood ratio was excluded by standardizing the cell concentrations to reflect the values in native blood. Statistical analysis included descriptive statistics, simple linear regression analysis, MannWhitney U-test, cumulative frequency plots and Smirnov two-sample test.Result: As expected, hemoglobin correlated with red blood cell concentration. Interestingly, mean platelet volume was associated with hemoglobin, red blood cell concentration and hematocrit. The platelet count was inversely associated with the parameters. Conclusion:The observed associations of cord blood hemoglobin with mean platelet volume and platelet count reflect the physiology of fetal hematopoiesis at term.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association of cord blood platelet count and volume with hemoglobin in healthy term infants

et al. 2010

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“…A média menor nas amostras conservadas sob temperatura ambiente provavelmente se deveu ao fato de que, em temperaturas mais elevadas, a lise de leucócitos é maior. Estudando a influência do tempo e da temperatura de armazenagem na conservação sanguínea em três equipamentos distintos, IMERI et al (2008) detectaram diminuição no número de leucócitos na temperatura ambiente, no tempo 48 horas, em um dos três equipamentos utilizados, enquanto nos resultados obtidos nos outros dois equipamentos, não foi observada diferença. COHLE et al (1981) a h na temperatura ambiente e diminuição na 12 a h na temperatura de 2 a 8°C.…”

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Concentração de anticoagulante, tempo e temperatura de armazenagem sobre os parâmetros hematológicos no hemograma automatizado

et al. 2010

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O estudo teve como objetivo identificar os efeitos do tempo de estocagem, da temperatura de armazenamento e da quantidade de anticoagulante sobre parâmetros hematológicos de cães. Foram utilizadas amostras do sangue de dez cães de raças variadas, clinicamente hígidos. As alíquotas foram colhidas com 1,8mg; 3,6mg; 7,2mg e 14,4mg de ácido etilenodiaminotetracético (EDTA) por mL de sangue, distribuídas em dois grupos: de 2°C a 8°C e temperatura ambiente. Após a coleta, foram avaliadas em quatro tempos: 0, 12, 24 e 48 horas. Usando um contador automático de células, foram avaliados leucócitos, eritrócitos, hemoglobina, hematócrito, volume corpuscular médio (VCM), índice de anisocitose eritrocitária (RDW), plaquetas e plaquetócrito (PCT). O valor do VCM diminuiu nas maiores concentrações de EDTA (7,2mg mL-1 e 14,4mg mL-1), com decréscimo de 2,36% na maior concentração. A temperatura e o tempo de armazenagem também ocasionaram alteração nesse parâmetro, ou seja, houve decréscimo no tempo 12 horas à temperatura de 2 a 8°C e aumento nos tempos 24 e 48 horas à temperatura ambiente (P<0,05). A temperatura de conservação influenciou discretamente a contagem de leucócitos e eritrócitos, que apresentaram valores menores na temperatura ambiente. Hemoglobina, hematócrito, plaquetas e PCT não apresentaram alteração significativa. As alterações observadas não comprometeram os resultados obtidos no contador automático, mostrando que as amostras sanguíneas, conservadas por 48 horas, mantiveram-se em boas condições para análise, principalmente quando armazenadas à temperatura de 2 a 8°C.

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“…However, some studies, which were conducted using refrigerated temperature show that WBC, RBC, and HGB parameters, were constant up to 72 h [21,22]. Only Ashenden et al recently confirmed that haemoglobin was stable for at least 168 h using the Sysmex XT-2000i instrument, when maintained temperature between 4°C and 6°C [18].…”

Section: Discussionmentioning

confidence: 99%

Evaluation and Comparison of Stability and Reliability of CBC Parameters Determined by Using Automatic Celltac G MEK-9100 Hematology Analyzer during Extended Storage at 4°C

Hussain¹,

Mehmood²,

Arshad³

et al. 2018

J Clin Res Bioeth

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Stability of hematological analytes depends on the hematology analyser used: A stability study with Bayer Advia 120, Beckman Coulter LH 750 and Sysmex XE 2100

Cited by 86 publications

References 10 publications

Association of cord blood platelet count and volume with hemoglobin in healthy term infants

Association of cord blood platelet count and volume with hemoglobin in healthy term infants

Concentração de anticoagulante, tempo e temperatura de armazenagem sobre os parâmetros hematológicos no hemograma automatizado

Evaluation and Comparison of Stability and Reliability of CBC Parameters Determined by Using Automatic Celltac G MEK-9100 Hematology Analyzer during Extended Storage at 4°C

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