Stability of fusaproliferin, a mycotoxin fromFusarium spp

Ritieni, Alberto; Monti, Simona Maria; Moretti, Antonio; Logrieco, A.; Gallo, Monica; Ferracane, Rosalia; Fogliano, Vincenzo

doi:10.1002/(sici)1097-0010(199909)79:12<1676::aid-jsfa419>3.0.co;2-5

Cited by 14 publications

(5 citation statements)

References 16 publications

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“…The calculated recovery of each mycotoxin was: BEA 74.6%, FP 56.1%, ENN-B 66.5%, ENN-B 1 62.5%, and 58.6% for ENN-A 1 . Although chloroform did not give the best recovery (Ritieni et al, 1998), this choice was balanced by the advantage of a fast separation of the organic phase from the polar one, thus allowing a rapid concentration of samples without thermal stress. Retention times recorded for authentic standards were FP 5.94 min, BEA 8.64 min, ENN-B 9.81 min, ENN-B 1 10.43, and 11.26 min for ENN-A 1 (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Beauvericin, Enniatins, and Fusaproliferin by High Performance Liquid Chromatography

Monti

Fogliano

Logrieco

et al. 2000

J. Agric. Food Chem.

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A rapid, sensitive and inexpensive HPLC method for routine screening of beauvericin, fusaproliferin, and enniatin B(1), A(1), and B has been optimized. Detection limits were determined, ranging between 0. 5 and 3.6 ng according to the compound obtained after spiking samples with each mycotoxin at 10-56 microg/mL concentration range; recoveries averaging from 56 to 74% were obtained. LC-MS conditions for enniatin analyses by API electrospray technique were set up, this allowing a unique identification of three different enniatins.

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Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Beauvericin, Enniatins, and Fusaproliferin by High Performance Liquid Chromatography

Monti

Fogliano

Logrieco

et al. 2000

J. Agric. Food Chem.

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“…Until now, fusaproliferin has been analyzed by using highperformance liquid chromatography (HPLC) with ultraviolet (UV) detection and/or thin-layer chromatography (TLC) (6)(7)(8)(18)(19)(20). In recent years, HPLC combined with mass spectrometric detection have also been used in several studies (21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

“…The extraction of the toxin has been performed with solvents of different polarities ranging from 99.5% methanol (8) to chloroform (23). The purification steps published include just filtration or liquid-liquid partitioning (6)(7)(8)(18)(19)(20)(21)(22)(23). This might decrease the sensitivity of the method, because the impurities still present in the sample can interfere with the detection of the analytes.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Fusarium Mycotoxins Fusaproliferin and Trichothecenes in Grains Using Gas Chromatography−Mass Spectrometry

Jestoi

Ritieni

Rizzo

2004

J. Agric. Food Chem.

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A method is described using gas chromatography-mass spectrometry (GC-MS) for the simultaneous detection of the Fusarium mycotoxins fusaproliferin and seven trichothecenes from grains. Sample purification of the raw extract was carried out with commercial solid phase extraction columns, and the recovery of the more polar analytes was increased by rinsing the column with acetonitrile. A significant matrix effect was found for the analysis of fusaproliferin and trichothecenes; thus, the calibrants should be prepared in a blank matrix. The response was linear in the range used. The mean recovery for fusaproliferin was 60.4 or 62.9%, depending on the spiking level. With respect to the trichothecenes, the recovery was generally higher (70.2-125.3%). The method proved to be repeatable for the analysis of fusaproliferin and trichothecenes. The limit of detection for fusaproliferin in the blank matrix mixture was 50 microg/kg, and that for trichothecenes was 5-15 microg/kg. Thirty-eight Finnish grain samples were analyzed for fusaproliferin and trichothecenes with the method developed. Fusaproliferin was not detected in any of the samples. The mean levels of deoxynivalenol, 3-acetyldeoxynivalenol, nivalenol, HT-2 toxin, and T-2 toxin in Finnish grain samples were 272, 17, 150, 40, and <20 microg/kg, respectively.

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“…The absolute stereochemistry of the compound was confirmed by Santini et al in 1996 [21]. FUS is also produced by Fusarium subglutinans and fifteen other ex-type strains of Fusarium species [22,23]. FUS produced a toxic effect on Artemia salina , insect cells, and human B lymphocytes [24].…”

Section: Introductionmentioning

confidence: 94%

Fusaproliferin, a Fungal Mycotoxin, Shows Cytotoxicity against Pancreatic Cancer Cell Lines

et al. 2018

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As a part of our ongoing research on endophytic fungi, we have isolated a sesterterpene mycotoxin, fusaproliferin (FUS), from a Fusarium solani strain, which is associated with the plant Aglaonema hookerianum Schott. FUS showed rapid and sub-micromolar IC50 against pancreatic cancer cell lines. Time-dependent survival analysis and microscopy imaging showed rapid morphological changes in cancer cell lines 4 h after incubation with FUS. This provides a new chemical scaffold that can be further developed to obtain more potent synthetic agents against pancreatic cancer.

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Stability of fusaproliferin, a mycotoxin fromFusarium spp

Cited by 14 publications

References 16 publications

Simultaneous Determination of Beauvericin, Enniatins, and Fusaproliferin by High Performance Liquid Chromatography

Simultaneous Determination of Beauvericin, Enniatins, and Fusaproliferin by High Performance Liquid Chromatography

Analysis of the Fusarium Mycotoxins Fusaproliferin and Trichothecenes in Grains Using Gas Chromatography−Mass Spectrometry

Fusaproliferin, a Fungal Mycotoxin, Shows Cytotoxicity against Pancreatic Cancer Cell Lines

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