Simultaneous Determination of Beauvericin, Enniatins, and Fusaproliferin by High Performance Liquid Chromatography

Monti, Simona Maria; Fogliano, Vincenzo; Logrieco, A.; Ferracane, Rosalia; Ritieni, Alberto

doi:10.1021/jf990373n

Cited by 43 publications

(28 citation statements)

References 22 publications

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“…We used all buffers and DNA modifying enzymes following either the manufacturer's instructions or standard protocols (46). EcoRI primers used in the final specific PCR amplifications were labeled with [␥- 33 P]ATP (PerkinElmer, Shelton, Conn.).…”

Section: Methodsmentioning

confidence: 99%

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Species Diversity of and Toxin Production by Gibberella fujikuroi Species Complex Strains Isolated from Native Prairie Grasses in Kansas

Leslie

Zeller

Logrieco

et al. 2004

Appl Environ Microbiol

104

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Fusarium species from agricultural crops have been well studied with respect to toxin production and genetic diversity, while similar studies of communities from nonagricultural plants are much more limited. We examined 72 Fusarium isolates from a native North American tallgrass prairie and found that Gibberella intermedia (Fusarium proliferatum), Gibberella moniliformis (Fusarium verticillioides), and Gibberella konza (Fusarium konzum) dominated. Gibberella thapsina (Fusarium thapsinum) and Gibberella subglutinans (Fusarium subglutinans) also were recovered, as were seven isolates that could not be assigned to any previously described species on the basis of either morphological or molecular characters. In general, isolates from the prairie grasses produced the same toxins in quantities similar to those produced by isolates of the same species recovered from agricultural hosts. The G. konza isolates produce little or no fumonisins (up to 120 g/g by one strain), and variable but generally low to moderate amounts of beauvericin (4 to 320 g/g) and fusaproliferin (50 to 540 g/g). Toxicity to Artemia salina larvae within most species was correlated with the concentration of either beauvericin or fusaproliferin produced. Organic isolates from some cultures of G. moniliformis were highly toxic towards A. salina even though they produced little, if any, beauvericin or fusaproliferin. Thus, additional potentially toxigenic compounds may be synthesized by G. moniliformis strains isolated from prairie grasses. The Fusarium community from these grasses appears to contain some species not found in surrounding agricultural communities, including some that probably are undescribed, and could be capable of serving as a reservoir for strains of potential agricultural importance.

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Section: Methodsmentioning

confidence: 99%

“…The amount of beauvericin and fusaproliferin was determined by HPLC as previously described by Monti et al (33) and Munkvold et al (36), respectively. In brief, HPLC analyses were performed using LC-10AD pumps and a diode array detector from Shimadzu (Kyoto, Japan).…”

Section: Methodsmentioning

confidence: 99%

Species Diversity of and Toxin Production by Gibberella fujikuroi Species Complex Strains Isolated from Native Prairie Grasses in Kansas

Leslie

Zeller

Logrieco

et al. 2004

Appl Environ Microbiol

104

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“…E3643) were purchased from Sigma Chemical Co. (St. Louis, Mo.). The enniatin mixture contained enniatin B (19%), enniatin B 1 (54%), enniatin A (3%), and enniatin A 1 (20%), as described by Monti et al (15). To extract beauvericin and enniatin, 10 g of each sample was ground and homogenized in a Ultraturrax model T25 basic from IKA Werke (Staufen, Germany) for 3 min at 13,500 rpm with 50 ml of methanol (HPLC grade).…”

Section: Methodsmentioning

confidence: 99%

“…Extracts were resuspended in 3 ml of methanol, prepurified once on a C 18 column (500 mg, 3 ml, 40 m) (Varian, Inc., Palo Alto, Calif.), concentrated to 1 ml, and filtered through an Acrodisk filter (pore size, 0.22 m) (Millipore, Jonezawa, Japan) before HPLC analysis. Beauvericin and enniatin (B, B 1 , and A 1 ) analyses were performed as described by Monti et al (15) with minor modifications. HPLC analyses were performed using LC-10AD pumps and a diode array detector from Shimadzu (Kyoto, Japan).…”

Section: Methodsmentioning

confidence: 99%

Occurrence of Beauvericin and Enniatins in Wheat Affected by Fusarium avenaceum Head Blight

Logrieco¹,

Rizzo

Ferracane

et al. 2002

Appl Environ Microbiol

Self Cite

224

102

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We evaluated Fusarium contamination and the levels of hexadepsipeptide mycotoxins in 13 wheat samples affected by head blight in Finland. Fusarium avenaceum was the dominant species (91%) isolated from all samples, but isolates of F. culmorum (4%), F. tricinctum (3%), and F. poae (2%) also were recovered. Beauvericin (0.64 to 3.5 g/g) was detected in all 13 samples. Enniatin B (trace to 4.8 g/g) was detected in 12 samples, enniatin B 1 (trace to 1.9 g/g) was detected in 8 samples, and enniatin A 1 (trace to 6.9 g/g) was detected in 10 samples. Ten of 13 strains of F. avenaceum and 2 strains of F. poae and F. tricinctum produced beauvericin in culture on rice (trace to 70, 9.4, and 33 g/g, respectively). All strains also produced enniatins (trace to 2,700 g/g). This is the first report on the natural cooccurence of beauvericin and enniatins in wheat infected predominantly by F. avenaceum.

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“…Some of the newer studies also point to the presence of other Fusarium toxins, for example deoxynivalenol (36), beauvericin (BEA), enniatins (ENNs), nivalenol (NIV), fusaroproliferin (FUS), and 3-acetyl-deoxynivalenol (3-AcDON) in environmental samples (37,38).…”

Section: Zearalenone In Cereal Samplesmentioning

confidence: 99%

Zearalenone Contamination of the Aquatic Environment as a Result of its Presence in Crops / Pojava Mikotoksina U Vodenom Okolišu Zbog Njihove Prisutnosti U Usjevima

Waśkiewicz

Gromadzka

Bocianowski

et al. 2012

Archives of Industrial Hygiene and Toxicology

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The aim of this study was to establish a relation between zearalenone contamination of crops in the Polish province of Wielkopolska and its occurrence in aquatic ecosystems close by the crop fi elds. Water samples were collected from water bodies such as drainage ditches, wells, or watercourses located in four agricultural areas. Moreover, control water samples were collected from the Bogdanka river, which was located outside the agricultural areas and near an urban area. Cereal samples were collected in the harvest season from each agricultural area close to tested water bodies. Zearalenone (ZEA) was found in all water and cereal samples. The highest concentrations were recorded in the postharvest season (September to October) and the lowest in the winter and spring. Mean ZEA concentrations in water ranged between 1.0 ng L -1 and 80.6 ng L -1 , and in cereals from 3.72 ng g -1 to 28.97 ng g -1 . Our results confi rm that mycotoxins are transported to aquatic systems by rain water through soil.

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Simultaneous Determination of Beauvericin, Enniatins, and Fusaproliferin by High Performance Liquid Chromatography

Cited by 43 publications

References 22 publications

Species Diversity of and Toxin Production by Gibberella fujikuroi Species Complex Strains Isolated from Native Prairie Grasses in Kansas

Species Diversity of and Toxin Production by Gibberella fujikuroi Species Complex Strains Isolated from Native Prairie Grasses in Kansas

Occurrence of Beauvericin and Enniatins in Wheat Affected by Fusarium avenaceum Head Blight

Zearalenone Contamination of the Aquatic Environment as a Result of its Presence in Crops / Pojava Mikotoksina U Vodenom Okolišu Zbog Njihove Prisutnosti U Usjevima

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