Abstract. The toxicity of zinc oxide nanoparticles (nZnO) with particle size Δ 50 = 20 nm was evaluated according to the degree of toxicity of the aqueous disperse system (DS) with biological testing methods using a set of test organisms representing the major trophic levels.We observed the influence of the concentration degree of nZnO on toxic effects level on the fluorescence of the bacterial biosensor "Ekolyum-13", the chemotactic response of ciliates Paramecium caudatum, the rate of growth of unicellular algae Chlorella vulgaris Bayer, mortality of entomostracans Daphnia magna Straus and fish Danio rerio. The detected values of L(E)C 50 are: for biosensor "Ekolyum-13" -0.30 mg/L, for ciliates Paramecium caudatum -0.14 mg/L, for Chlorella vulgaris Bayer -0.17 mg/L and for Daphnia magna Straus -0.52 mg/L. No toxicity of nZnO was detected in relation to fish Danio rerio, L(E)C 50 > 100 mg/L. In assessing the maximum effect of nZnO according to GHS and EU Directive 93/67/ EEC, it is assigned to dangerous substances with a high degree of toxicity "Acute toxicity 1".
IntroductionDevelopment of nanotechnology, growing production volumes of fine disperse materials (FDM), the exponential growth in the number of products containing engineered nanoparticles (NP), on the one hand, opens up new possibilities of using FDM in biomedicine, pharmacology, food production, in addressing environmental and agricultural issues. On the other hand, the unique properties of FDM may carry a potential risk to the environment.The need to determine the safety of FDM, approved by regulatory documents of Russia and OECD, ISO, EU and the Environmental Protection Agency [1, 2], arises from the large volumes of production and a wide range of application of FDM.Despite the fact that currently the volume of industrial production of various NP is more than 100,000 tons a year, studies on the safety of nanotechnologies and nanoproducts are scattered, fragmentary, incomplete and significantly far behind the FDM production rate growth and expansion of their scope of usage. To date, most of the published papers are devoted to the study of biological effects of the most popular metal oxides NP. According to Bondarenco et. al. [3] among metal oxides NP, the NP of zinc oxide (nZnO) hold the third place in terms of volume of production (nSiO2 -55,000 tons/year, nTiO2 -3000 t/year, nZnO -550 tons/year). The wide range of applications of nZnO in the field of cosmetology, medicine, materials science [4,5,6,7] is due to its anti-bacterial, surfactant and catalytic properties [8,9]. The presence of biological effects when NP contact with representatives of the biota makes the research on the biological effects of FDM, identifying risks to the environment and ensuring biosafety as a necessary component in the development of