Stability-indicating spectrofluorimetric method with enhanced sensitivity for determination of vancomycin hydrochloride in pharmaceuticals and spiked human plasma: Application to degradation kinetics

Abstract

Based on investigating the relative fluorescence intensity of vancomycin hydrochloride (VCM) in methanol, a simple, highly sensitive, time-saving and specific spectrofluorimetric method was developed and validated. VCM fluorescence was measured at 335 nm when excited at 268 nm. Excellent linearity is obeyed in the concentration range 1–100 ng/mL with a detection limit of 5.94 pg/mL, a quantitation limit of 18.03 pg/mL and a very good correlation coefficient ( r = 0.9999). Our method was applied to analyze VCM in pharmaceuticals as well as spiked human plasma. Moreover, VCM stability was studied when exposed to various degradation conditions such as oxidative, alkaline as well as acidic stress. Acidic and alkaline degradation kinetics of VCM was studied for the first time. The degradation follows pseudo-first-order kinetics. The apparent rate constants and half-life times were calculated. The Arrhenius equation was assessed and the activation energies of the degradation were also calculated. The developed method can be easily applied in quality control laboratories due to its sensitivity, specificity, simplicity and low cost.

Analytical methods for the determination of certain antibiotics used in critically ill patients

“…Specific spectrofluorimetric method was developed and validated. Vancomycin fluorescence was measured at 335 nm when excited at 268 nm where Vancomycin stability was studied when exposed to various degradation conditions such as oxidative, alkaline as well as acidic stress [108] .…”

Cobalt-modulated dual emission carbon dots for ratiometric fluorescent vancomycin detection

“…2 Therefore, therapeutic drug monitoring of vancomycin is necessary. Various analytical methods, including high performance liquid chromatography (HPLC), [8][9][10] electrochemical detection, 11,12 immunoassay, 13,14 UV-vis spectrophotometric 15,16 and uorometric methods [17][18][19][20][21][22][23] have been employed to quantify vancomycin in biological samples.…”

Spider diagram with greenness evaluation metrics for assessing the new synchronous spectrofluorimetric determination of bicalutamide and resveratrol in human plasma

“…The spectrofluorimetric method is characterized by its high sensitivity, selectivity, and simplicity [18,19]. However, synchronous fluorescence spectroscopy (SFS) mode is necessary to use when examining combinations with overlapping spectra.…”