Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Albendazole and Ivermectin in Pharmaceutical Dosage form

Bhavya, B.; Nagaraju, P.; Mounika, V.; Priyadarshini, G. Indira

doi:10.5958/2231-5675.2017.00002.3

Cited by 20 publications

(6 citation statements)

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“…13 The literature described quite a few analytical methods for the estimation of ivermectin and praziquantel in combination with other drugs. These methods are based on techniques, such as ultraviolet spectroscopy, 16 diffuse reflectance spectroscopy, 16 high performance liquid chromatography, [17][18][19][20][21] high performance thin layer chromatography 22 and high performance liquid chromatography with tandem mass spectrometry. 23 To the best of our knowledge, till now only one HPLC method for the simultaneous quantification of ivermectin and praziquantel in Equimax paste is reported.…”

Section: Discussionmentioning

confidence: 99%

Chromatographic Quantification of Ivermectin and Pranziquantel in the Tablets Using Stability Indicating RP-HPLC Method

Devaka

Rao

2019

Pharm Sci

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Background: A new stability indicating RP-HPLC based assay method was developed to quantify ivermectin and praziquantel simultaneously and applied effectively to tablets. Methods: The simultaneous assay of ivermectin and praziquantel by RP-HPLC was done using an YMC C18 (250 mm × 4.6 mm, 5 µm) column with a mobile phase mixture of 0.1M disodium hydrogen phosphate (pH 4.5) and acetonitrile (55:45, v/v) using a isocratic flow rate of 1.0 ml/min and measured at 242 nm using photodiode array detector. All parameters were validated following the ICH guiding principles. The method was applied to quantify ivermectin and praziquantel simultaneously in tablets. Results: The retention values of ivermectin and praziquantel were 3.465 min and 4.468 min, respectively. The method’s linearity was found to be 1-3 µg/ml (ivermectin) and 25-75 µg/ml (praziquantel). The limit of detection was 0.010 µg/ml (ivermectin) and 0.046 µg/ml (praziquantel); limit of quantification was 0.033 µg/ml (ivermectin) and 0.155 µg/ml (praziquantel). The percent relative standard deviation of ivermectin and praziquantel was ˂1.0%. The percent assay was 99.51% and 99.20% for ivermectin and praziquantel, respectively. In tablets, the percent recovery of ivermectin and praziquantel was 99.60% and 99.38% with a percent relative standard deviation value of 0.353% and 0.106%, respectively. Stability indicating capability of the method was demonstrated through the stress degradation studies. Conclusion: The developed method was proved to be selective, precise and accurate for the quality control of ivermectin and praziquantel in tablets.

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Section: Discussionmentioning

confidence: 99%

Chromatographic Quantification of Ivermectin and Pranziquantel in the Tablets Using Stability Indicating RP-HPLC Method

Devaka

Rao

2019

Pharm Sci

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“…10 mg like ivermectin but also 1 mg like albendazole operating conventional seemed to be prepared by weighing and then were transmitted it into a 10ml freshly washed volumetric lask, add approximately 2ml of dissolution medium but rather species threatened with extinction to disperse it effectively making volume turned up to that same record with almost the same diluents [10].…”

Section: Linearity Time To Prepare Of Inventory Levels Solutionmentioning

confidence: 99%

Test

Injeti¹

2022

Fut J. Pharm & H. Sci

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“…The performance of the force degradation testing will be depending on the type of drug product and the individual drug substance. In this view the analysis of molnupiravir done in under variety of conditions, develop the LC method for separation the drug substance from the degradation products formed during forced degradation studies under ICH suggested conditions (thermal stress, hydrolysis, photolysis, and oxidation) [6][7][8][9][10][11][12][13][14][15][16][17]. These stress study experiments to provide good information about drug substance stability and which can help to establishment of method validation of drug substance [18].…”

Section: Fig 1 Molnupiravir Structurementioning

confidence: 99%

Method Development and Validation of a Specific Stability Indicating RP-HPLC Method for Molnupiravir API

Kumar

Babu

Rao

et al. 2021

JPRI

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A simple, accurate, isocratic stability indicating RP‐HPLC method was developed for the determination of Molnupiravir in bulk drug. This RP-HPLC was achieved on “Waters 2695 using an Agilent Zorbax Eclipse C18 (250 mm × 4.6 mm × 5 µm)” column with the mobile phase consisting of 30 mM, ammonium phosphate monobasic and Methanol in the ratio of 47:53 %v/v. The stress testing of Molnupiravir was carried out under acidic, alkaline, oxidative, thermal, and photolytic conditions and formed degradation products were well resolved from Molnupiravir API (active pharmaceutical ingredient). The suggested approach was validated according to ICH (International Council on Harmonisation) principles, and all of the validation parameters' findings were within acceptable limits. The method was proven to be appropriate for quality control of Molnupiravir in bulk and pharmaceutical dose forms, as well as stability testing.

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Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Albendazole and Ivermectin in Pharmaceutical Dosage form

Cited by 20 publications

References 0 publications

Chromatographic Quantification of Ivermectin and Pranziquantel in the Tablets Using Stability Indicating RP-HPLC Method

Chromatographic Quantification of Ivermectin and Pranziquantel in the Tablets Using Stability Indicating RP-HPLC Method

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Method Development and Validation of a Specific Stability Indicating RP-HPLC Method for Molnupiravir API

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