Stability-indicating HPLC Method for Simultaneous Determination of Atenolol, Aspirin, Lisinopril and Simvastatin in Bulk and Tablets

Abstract

Rao and Gowrisankar: Stability-indicating HPLC Method for cardiovascular drugsA simple, accurate, specific and rugged reverse phase liquid chromatographic method was developed for the simultaneous estimation of atenolol, lisinopril, aspirin and simvastatin in bulk and tablet dosage form. A reverse phase gradient program has been developed to separate all the four active ingredients. A gradient programming has been done using 0.05 M Phosphate buffer pH 2.5 adjusted with dilute phosphoric acid, acetonitrile in the ratio 70:30 from 0 min to 10 min, further increase the acetonitrile ratio from 30 to 70 from 10 min to 20 min, on a Hypersil BDS C8 (250×4.6 mm, 5 µ) with a flow rate 1 ml/min, monitored at 232 nm. The mean retention times of atenolol, lisinopril, aspirin and simvastatin were found to be 3.9, 5.8, 9.5 and 18.3 min, respectively. The linearity was established for atenolol 12.5 to 75 µg/ml, lisinopril 2.5 to 15 µg/ml, aspirin 18.75 to 112.5 µg/ml, simvastatin 5 to 30 µg/ml. The proposed method was validated in terms of linearity, range, accuracy, precision, specificity, robustness and ruggedness and the method was successfully applied to the estimation of atenolol, lisinopril, aspirin and simvastatin in combined tablet dosage form.

Stress Degradation of Lisinopril Dihydrate in Different Aqueous Media

“…Based on the presented, it is very difficult to find out which is the ideal pH value that will ensure that LSDH and its impurities are presented only in molecular or ionic form. By reviewing the literature [11,[13][14][15]17] various SIM methods with a range of pH values from 2.2 to 7.1 are developed, which is still quite a wide range. By using the estimate obtained in the Marvin Sketch program, LSDH and most impurities at a pH of 3.0 -4.0 are ~ 100% in molecular form, and this pH value of 3.0 will be used to prepare the mobile phase in a chromatographic analysis of this stress study [11].…”

“…Based on the literature review [12][13][14][15][16][17], it can be seen that until now similar analyses with LSDH have not been performed. This is the first time that this type of experiment was carried out at two temperatures (25°C and 50°C) under the influence of various stress agents, where the stability and degradation profile of the test compound was determined based on kinetic parameters and Ea.…”

Two smart spectrophotometric methods for simultaneous determination of Lisinopril and Hydrochlorothiazide in binary mixtures

“…When ACE inhibitors are used at the same time, activation of the renin-angiotensin system is prevented, enhancing the effect of both therapies and decreasing their side effects as lower doses are needed [2,3]. LIS was previously analyzed by titrimetric [4], spectrophotometric [5][6][7][8][9][10][11][12][13][14], Spectrofluorimetric [15][16][17][18][19], chromatographic [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38], electrophoretic [39,40] and electrochemical methods [4,41,42]. Mixtures of LIS and HCT were analyzed simultaneously by spectrophotometric [43][44][45], chromatographic [21,22,29,<...>…”